Investigation of silk as a phantom material for ultrasound and photoacoustic imaging

Nguyen, Catherine; Edwards, Skye A.; Iorizzo, Tyler W.; Longo, Brooke N.; Yaroslavsky, Anna N.; Kaplan, David L.; Mallidi, Srivalleesha

doi:10.1016/j.pacs.2022.100416

Cited by 8 publications

(11 citation statements)

References 51 publications

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“…Moreover, recently Nguyen et al 20 . reported that silk‐based phantom materials have the advantage of not needing additional scattering materials like titanium dioxide.…”

Section: Resultsmentioning

confidence: 99%

“…Although this has guaranteed sufficient imaging contrast between soft and rigid materials, in the future, we aim to use customized materials to mimic the natural bone tissue characteristics as proposed by Tavakolian et al for a neonatal skull-mimicking phantom for PAI. 53 Moreover, recently Nguyen et al 20 reported that silkbased phantom materials have the advantage of not needing additional scattering materials like titanium dioxide. Just by changing silk concentration and other fabrication methods, the acoustic and optical properties of the phantoms can also be modulated.…”

Section: Discussionmentioning

confidence: 99%

“…14,15 Besides, a variety of other TMMs has been used to fabricate PAI phantoms, such as paraffin gel wax, 16,17 emulsions such as Intralipid, 18 glycerolin-polydimethylsiloxane (PDMS), 9 and polyvinyl chloride plastisol (PVCP) 19 Further developments in the field of TMMs for PAI, aimed to combine durability, long-term stability, as well as tunability of the acoustic and optical properties. To this end, TMMs such as silk protein-based hydrogels 20 and a copolymer-in-oil material that is formulated by the International Photoacoustic Standardization Consortium (IPASC) 3,[21][22][23][24] have been proposed. In particular, the copolymer-in-oil base material offers tunable acoustic and optical properties similar to soft tissue and has good durability characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…Further developments in the field of TMMs for PAI, aimed to combine durability, long‐term stability, as well as tunability of the acoustic and optical properties. To this end, TMMs such as silk protein‐based hydrogels 20 and a copolymer‐in‐oil material that is formulated by the International Photoacoustic Standardization Consortium (IPASC) 3,21–24 have been proposed. In particular, the copolymer‐in‐oil base material offers tunable acoustic and optical properties similar to soft tissue and has good durability characteristics.…”

Section: Introductionmentioning

confidence: 99%

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Development of a morphologically realistic mouse phantom for pre‐clinical photoacoustic imaging

et al. 2023

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BackgroundCharacterizations based on anatomically realistic phantoms are highly effective to perform accurate technical validation of imaging systems. Specifically for photoacoustic imaging (PAI), although a variety of phantom models with simplified geometries are reported, an unmet need still exists to establish morphologically realistic heterogeneous pre‐clinical phantoms. So the development of a mouse‐mimicking phantom can reduce the use of animals for the validation and standardization studies of pre‐clinical PAI systems and thus eventually translate the PAI technology to clinical research.PurposeHere we designed, developed, and fabricated a stable phantom that mimics the detailed morphology of a mouse, to be used as a realistic tool for PAI.MethodsThe mouse phantom, has been designed by using a combination of image modeling and 3D‐printing techniques. As a tissue‐mimicking material, we have used copolymer‐in‐oil‐based material that was recently proposed by the International Photoacoustic Standardization Consortium (IPASC). In particular, the anatomically realistic phantom has been modeled by using the real atlas of a mouse as a reference. The mouse phantom includes a 3D‐printed skeleton and the main abdominal organs such as the liver, spleen, and kidneys obtained by using doped copolymer‐in‐oil material with 3D‐printed molds. In addition, the acoustic and optical properties of the tissue‐mimicking material and the long‐term stability have been broadly characterized.ResultsFurthermore, our studies showed that the phantom is durable and stable for more than 200 days, under normal storage and repeated use. Fabrication protocol is easy to reproduce. As a result, the proposed morphologically realistic mouse phantom offers durability, material compatibility, and an unprecedented realistic resemblance to the actual rodents’ anatomy in PAI.ConclusionThis durable morphologically realistic mouse phantom would minimize the animal experiments in compliance with the 3R principle of Replacement, Reduction, and Refinement. To our knowledge, this is the first time an anatomically realistic heterogeneous mouse phantom has been proposed for PAI in pre‐clinical animal imaging and tested its durability over 200 days.

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“…Moreover, recently Nguyen et al 20 . reported that silk‐based phantom materials have the advantage of not needing additional scattering materials like titanium dioxide.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a morphologically realistic mouse phantom for pre‐clinical photoacoustic imaging

et al. 2023

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“…While several tissue phantoms possess such properties, they are impractical for our purposes due to the complexities in creation and size constraints. [31][32][33][34][35][36] Consequently, we opted for a format that allows for the use of a cost-effective and thin cover material.…”

Section: Fabrication Protocol Of a Testbed Setupmentioning

confidence: 99%

Testbed mimicking intracellular environment for optical-resolution photoacoustic microscopy

Nishimae,

Ishii,

Ogasawara

et al. 2024

Jpn. J. Appl. Phys.

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Photoacoustic imaging (PAI) represents an innovative method that utilizes optics for transmission and acoustic for sensing. Within the realm of PAI modalities, optical-resolution photoacoustic microscopy (OR-PAM) stands out for its ability to achieve high-resolution cellular and intracellular imaging. However, an optical testbed setup emulating the intracellular environment and accelerating the development of cellular-level OR-PAM systems has not been established. In this study, we present a testbed setup designed to mimic intracellular conditions. Assessment of this testbed setup efficacy involved embedding photoacoustic contrasts and imaging them using an OR-PAM system. The imaging results show that our testbed setup can provide instrumental in accurately measuring photoacoustic signals within an intracellular aqueous environment, avoiding risks of the contamination and degradation of the acoustic transducer.

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Multi-parametric Photoacoustic Imaging Combined with Acoustic Radiation Force Impulse Imaging for Applications in Tissue Engineering

Nguyen,

Chen,

Kaplan

et al. 2024

Ann Biomed Eng

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Investigation of silk as a phantom material for ultrasound and photoacoustic imaging

Cited by 8 publications

References 51 publications

Development of a morphologically realistic mouse phantom for pre‐clinical photoacoustic imaging

Development of a morphologically realistic mouse phantom for pre‐clinical photoacoustic imaging

Testbed mimicking intracellular environment for optical-resolution photoacoustic microscopy

Multi-parametric Photoacoustic Imaging Combined with Acoustic Radiation Force Impulse Imaging for Applications in Tissue Engineering

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