Investigation of Seven Different RNA Viruses Associated with Gastroenteritis in Children Under Five Years Old

Akhter, Shamim; Turegun, Buse; Kıyan, Mehmet; Gerçeker, Devran; Güriz, Haluk; Şahin, Fikret

doi:10.5578/mb.7374

Cited by 9 publications

(3 citation statements)

References 26 publications

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“…Overall, viruses were found as a sole pathogen in 53 % of the samples tested, while bacteria and parasites were detected in 13 % in both single and mixed infections. Data from our neighbouring countries Turkey, Greece and Albania (Akhter et al, 2014;Levidiotou et al, 2009;Fabiana et al, 2007) and also from Hungary, Spain and France (Jakab et al, 2009;Boga et al, 2004;Chikhi-Brachet et al, 2002) are concordant with our findings. Most data for Bulgaria are limited to the aetiology of bacterial and parasitic gastroenteritis (Parmakova et al, 2012(Parmakova et al, , 2013; http://www.ncipd.org/epidemiologicalbulletin).…”

Section: Discussionsupporting

confidence: 82%

Aetiology of acute paediatric gastroenteritis in Bulgaria during summer months: prevalence of viral infections

Mladenova

Steyer

et al. 2015

Journal of Medical Microbiology

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Paediatric acute gastroenteritis is a global public health problem. Comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections. Our study aimed to investigate the role of various pathogens in infantile diarrhoea in Bulgaria outside the classical winter epidemics of rotavirus and norovirus. Stool samples from 115 hospitalized children aged 0-3 years collected during summer months were tested for presence of 14 infectious agents -group A rotavirus, astrovirus, Giardia, Cryptosporidium and Entamoeba using ELISAs; norovirus by real-time RT-PCR; picobirnavirus and sapovirus by RT-PCR; adenovirus using PCR, and Salmonella, Shigella, Escherichia coli, Yersinia and Campylobacter using standard bacterial cultures. Infectious origin was established in a total of 92 cases and 23 samples remained negative. A single pathogen was found in 67 stools, of which rotaviruses were the most prevalent (56.7 %), followed by noroviruses (19.4 %), enteric adenoviruses (7.5 %), astroviruses (6.0 %), bacteria and parasites (4.5 % each) and sapoviruses (1.4 %). Rotavirus predominant genotypes were G4P[8] (46.3 %) and G2P[4] (21.4 %); for astroviruses, type 1a was the most common, while the GII.4/2006b variant was the most prevalent among noroviruses. Bacteria were observed in five cases, with Salmonella sp. as the most prevalent, while parasites were found in ten stool samples, with Giardia intestinalis in five cases. The results demonstrated high morbidity associated with viral infections and that rotavirus and norovirus remain the most common pathogens associated with severe gastroenteritis during summer months in Bulgaria, a country with a temperate climate, and significant molecular diversity among circulating virus strains.

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Section: Discussionsupporting

confidence: 82%

Aetiology of acute paediatric gastroenteritis in Bulgaria during summer months: prevalence of viral infections

Mladenova

Steyer

et al. 2015

Journal of Medical Microbiology

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“…In developing countries, enteric viruses such as rotaviruses, noroviruses, human enteric adenoviruses (HAdV), human astrovirus, and sapoviruses are the major cause of childhood diarrhoea (2,3). Bocavirus, aichivirus, and human parechovirus have recently been described in patients with diarrhoea, but their association with acute gastroenteritis has not been established yet (4,5).…”

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea

Çolak¹,

Bozdayı²,

Altay³

et al. 2017

Turk J Med Sci

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Background/aim: To determine the frequency, genotype distribution, and genetic relatedness of adenoviruses in children under 5 years old with diarrhoea and to investigate their distribution according to clinical findings, age, months, and seasons.Materials and methods: Stool samples were collected from 180 children with acute gastroenteritis who presented from July 2007 through June 2011 at the Ankara Training and Education Hospital. Stool samples were analysed by immune chromatographic test (ICT), enzyme immunoassay (EIA), and polymerase chain reaction (PCR). All adenovirus types were determined by nucleotide sequence analysis. A phylogenetic tree was constructed by Mega 6.0 using the neighbour-joining method.Results: Five percent of the samples were positive for adenovirus (9/180) by ICT, 6.1% (11/180) by EIA, and 13.9% (25/180) by PCR. Adenovirus gastroenteritis did not show any differences in age group, sex, month, or season. In this study, 16 (64%) of the PCR positive samples were AdV41, 6 (24%) were AdV40, 2 (8%) were AdV31, and 1 (4%) was AdV7, as determined by nucleotide sequencing. Conclusion:AdV31 and AdV7 were associated with gastroenteritis. Adenovirus serotypes showed a similarity of 80% (20/25) and 20% (5/25) with Asian and American serotypes, respectively.

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“…Because the sample size was small we did not make a statistical interpretation. There are three other reports from Ankara reporting 10% (15/150) 15 , 14.1% (141/1000) 16 and 16% (8/50) 17 positive results for noroviruses. Interestingly, during an outbreak in 2009 among military personnel in a USA air-base in İncirlik, Turkey, 16 samples from 37 samples were found positive for GII norovirus 18 .…”

Section: Resultsmentioning

confidence: 93%

Norovirüs enfeksiyonlarının Çanakkale'de ELİSA ve RT-PCR ile saptanması

Aksu¹,

Akçalı²

2016

Cukurova Medical Journal (Çukurova Üniversitesi Tıp Fakültesi Dergisi)

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AbstractÖz Purpose: Noroviruses are important agents of gastroenteritis worldwide in every age group. There is no large countrywide dataset available for norovirus infections in Turkey. This is the first investigation of noroviruses in Çanakkale, and we aimed to compare commercial ELISA and real time RT-PCR assays in clinical use. Material and Methods: Fecal samples from 92 clinical gastroenteritis cases were collected and stored at -20°C. Norovirus antigen was investigated using RidaScreen ELISA and norovirus GI and GII molecular detection made with RealStar Norovirus RT-PCR kit 2.0. Results: We found that 16 (17.4%) samples were positive for norovirus in our study group. Using ELISA, 10 samples were positive from 92 samples. In PCR analysis, internal control amplification failed for four samples. We found 15 of 88 samples positive for real time RT-PCR analysis; of these one was GI and 14 were GII. When we accepted PCR as the reference test, the sensitivity and specificity of ELISA were calculated as 60% and 98% respectively. Conclusion: Single cases of norovirus infection can be seen even in rural cities like Çanakkale. There is a need to set up diagnostic capabilities for norovirus infections. ELISA has a low sensitivity, but this method is cheaper than RT-PCR and is not affected by PCR inhibitors.

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Investigation of Seven Different RNA Viruses Associated with Gastroenteritis in Children Under Five Years Old

Cited by 9 publications

References 26 publications

Aetiology of acute paediatric gastroenteritis in Bulgaria during summer months: prevalence of viral infections

Aetiology of acute paediatric gastroenteritis in Bulgaria during summer months: prevalence of viral infections

Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea

Norovirüs enfeksiyonlarının Çanakkale'de ELİSA ve RT-PCR ile saptanması

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