Investigation of Sensitivity to AZD7762 in TripleNegative Breast Cancer (TNBC) with RBFOX2 Gene Expression as a Biomarker

Abstract

Objective: Triple negative breast cancer (TNBC) is one of the most metastatic, aggressive with poor prognosis types of breast cancers. There is currently no standard molecular-targeted treatment for TNBC. Therefore, new therapeutics should be developed. The aim of this study was to determine the effect of AZD7762 on several breast cancer cell lines and evaluate the RBFOX2 gene expression levels as a marker to show sensitivity to this drug. Materials and Methods:The cytotoxic effect of AZD7762 on breast cancer cell lines was determined by sulforhodamine B method. The expression levels of RBFOX2 gene were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The association between the IC50 values of the selected drug AZD7762 and RBFOX2 expression levels was evaluated by using Pearson r correlation analysis. Results:Although there was no significant difference between the IC50 values of TNBC and non-TNBC groups, it was determined that TNBC cell lines tended to be more sensitive to AZD7762. In addition, it was obvious that increasing levels of RBFOX2 expression were detected in cells that showed more sensitivity to AZD7762. Conclusion:It was concluded that the RBFOX2 gene can be used as a biomarker to show AZD7762 efficiency. Further studies are needed to investigate the potential signaling mechanisms that are associated with the effect of AZD7762.