Investigation of Sensitivity of the Allium cepa Test as an Alert System to Evaluate the Genotoxic Potential of Soil Contaminated by Heavy Metals

Pohren, Roberta de Souza; Costa, Thatiana Cappi da; Vargas, Vera Maria Ferrão

doi:10.1007/s11270-013-1460-1

Cited by 23 publications

(7 citation statements)

References 34 publications

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“…In the present study, we evaluated the genotoxic potential of AA in exposed mice bone marrow, using CAs, MI, and micronucleus assay. Chromosomal aberrations are considered the end result of genotoxic effects of various physical and chemical agents and are also estimates of exposure of various organisms to different physical and chemical agents (de Souza Pohren et al 2013). The increased CAs could be attributed to either an increase results from this study showed a statistically significant difference in the frequencies of micronuclei induction in mice bone marrow exposed to AA when compared to the control.…”

Section: Discussionmentioning

confidence: 59%

Genotoxic effects of acrylamide in mouse bone marrow cells

Algarni

2018

Caryologia

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Acrylamide (AA) is a human carcinogen generated in carbohydrate-rich foodstuffs upon heating. It has been shown to cause neurotoxic effects in humans, and genotoxic and carcinogenic effects in laboratory animals. The aim of this study was to assess the effects of AA on the frequency of chromosomal aberrations (CA), frequency of micronuclei (MN) and mitotic index (MI). Four groups of four mice, each weighing approximately 20-25 g, were orally administered AA once daily for 30 days with doses of 0, 2, 4, 8, 16, and 32 mg kg -1 body weight of AA. The control group consisted of four animals orally administered deionized water. Bone marrow was collected 24 h after the last treatment following standard protocols. AA significantly increased (p < 0.05) the number of CA and the frequency of micronucleated cells and decreased the MI in exposed groups compared to the negative control. The results obtained in this study further support that AA induces genotoxicity in mouse bone marrow cells.

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Section: Discussionmentioning

confidence: 59%

Genotoxic effects of acrylamide in mouse bone marrow cells

Algarni

2018

Caryologia

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“…MI is an indicator of adequate cell proliferation and is an important parameter to evaluate the negative influence of extracts in the cell cycle [10,18]. The comparison between MI values of the treatment groups and the negative control group can be used to infer about the development of the exposed organism.…”

Section: Resultsmentioning

confidence: 99%

Mutagenic Effect of Three Invasive Species through Allium Cepa Bioassay

Costalonga¹,

Dutra²,

Batitucci³

2017

JPP

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Abstract:Invasive alien species are a global threat to biodiversity that affects protected areas around the world. The occupation of new environments by these plants is a problem to be solved and it is essential to investigate all the aspects that allow this successful to find solutions to this question, such as its mutagenic effects. Thus, this study aimed to evaluate the mutagenic effect of leaves extracts of Acacia mangium Willd, Artocarpus heterophyllus Lam and Eriobothrya japonica (Thunb.) Lindl through Allium cepa bioassay. For this, A. cepa seeds were submitted to continuous and discontinuous (acute and chronic) treatments in medium with water (negative control) or four concentration of each extract (1, 5, 10 and 50 mg/mL). The mitotic index was affected at all concentrations of three extracts tested in all treatments, continuous and discontinuous. Aneugenic effects were not related to any treatment tested. E. japonica extract induced clastogenic effects at 1, 5 and 10 mg/mL in continuous treatment, 5 and 10 mg/mL in acute discontinuous treatment and at 10 mg/mL in chronic discontinuous treatment. Clastogenic effect was also observed at 10 mg/mL of A. heterophyllus extract in continuous and acute discontinuous treatments.

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“…PTEs (metalloid/heavy metals) bind with biomolecules in the tissues of living beings and disturb their genetic makeup, but as far as determination of effect of an environmental sample on genetic makeup of an organism or human population is concerned, quantitative estimation of PTEs solely may not serve the purpose. Therefore, different bioassays are required to determine the genotoxic potential of environmental samples (Bianchi et al 2015;de Souza Pohren et al 2013;Manzano et al 2015). In the present work, two bioassays, i.e.…”

Section: Genotoxicity Analysis Of Surface Water Samplesmentioning

confidence: 99%

Potentially toxic elements in river water and associated health risks in Ropar Wetland, India and its vicinity

Sharma

Nagpal

Kaur

2021

Int. J. Environ. Sci. Technol.

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In the present study, quality of surface water from five different locations on the banks of river Sutlej in Ropar wetland was determined by estimation of physico-chemical parameters; water quality indices; contents of arsenic, cadmium, cobalt, chromium, copper, iron, manganese, lead and zinc; determination of genotoxic potential of water samples using Allium cepa root chromosomal aberration assay and plasmid pBR322 DNA nicking assay; and assessment of associated possible human health risks for adults and children. River water was slightly alkaline, moderate to very hard, non-saline, genotoxic and unsuitable for both drinking and irrigation. Among 20 surface water samples analysed, contents of Cd, Cr, Cu, Fe, Mn and Pb in 11, 7, 3, 15, 14 and 13 samples exceeded the safe limits (mg/L) of 0.003, 0.05, 0.05, 0.30, 0.10 and 0.01, respectively. Ingestion of surface posed higher health risk than dermal contact, where Co was major contributor to mean hazard index (3.26-10.72 for adults and 4.22-13.87 for children). Hazard index for dermal contact in both age groups was < 1.00. Cr was found to be primarily responsible for high total cancer risk posed by both routes of exposure, i.e. ingestion (6.83E−04 to 2.03E−03 for adults and 7.58E−05 to 2.25E−04 for children) and dermal contact (5.15E−05 to 2.02E−04 for adults and 6.23E−06 to 2.44E−05 for children). Prolonged exposure to contaminated surface water may elevate the human health risk in the study area; therefore, pre-treatment of river water is necessary before its utilization by human population of the study area.

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Investigation of Sensitivity of the Allium cepa Test as an Alert System to Evaluate the Genotoxic Potential of Soil Contaminated by Heavy Metals

Cited by 23 publications

References 34 publications

Genotoxic effects of acrylamide in mouse bone marrow cells

Genotoxic effects of acrylamide in mouse bone marrow cells

Mutagenic Effect of Three Invasive Species through Allium Cepa Bioassay

Potentially toxic elements in river water and associated health risks in Ropar Wetland, India and its vicinity

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