Investigation of<scp>L</scp>(+)-Ascorbic acid with Raman spectroscopy in visible and UV light

Berg, Rolf W.

doi:10.1080/05704928.2014.952431

Cited by 45 publications

(27 citation statements)

References 87 publications

(162 reference statements)

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“…It is seen that there is a sharp absorption peak at ∼ 185.8 nm and an absorption region between ∼ 215 nm and ∼ 300 nm. There is a good consistency between the obtained UV absorption spectra ( Figure 2) and UV absorption spectra given in [57] for wavelengths bigger than 200 nm (no data seems to exist for λ ˂ 200 nm). It was concluded in [48] that "AA absorbs between 210 and 290 nm with a maximum at ∼ 247 nm for concentrated aqueous solutions."…”

Section: Far-uv Absorption Spectra Of Ascorbic Acid (Aa)supporting

confidence: 65%

Water-SARS-CoV-2 Interaction-Based Mechanism Inhibiting Virus Attachment to Host Cells

Shalatonin

2020

Preprint

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Many studies showed that the enveloped viruses, including coronaviruses, HIV-1, and influenza inactivate significantly faster in water than the non-enveloped viruses. It looks that there is a certain mechanism controlling this phenomenon. However, the epidemic spread of SARS-CoV-2 indicates that this virus – water interaction mechanism is not effective enough to fully inhibit coronavirus reproduction. We hypothesized that a spatially extended layer of the ordered water molecules, formed around CoV due to the spike’s glycans – surrounding water interaction acts as a buffer inhibiting CoV motion and its attachment to the host cell receptor. There is experimental evidence that water molecules while interacting with glucans experience the long-range ordering and repulsive forces. Our findings revealed new features that can promote its interaction. It was shown that the glycans and water molecules have the same far ultraviolet (UV) absorbance peak at ~185 nm. This peak is a manifestation of the still little-known physical properties possessed by hydroxyl (OH) groups, including those contained in glycans and water molecules. Many studies show that the carbohydrate hydroxyl groups are a key element in the long-range antifreeze glycoproteins activity which is closely correlated with our issue. To further increase ice inhibition, a sugar-based (usually trehalose) water solution, further slowing down the water dynamics is commonly used. Our experiments with sugar-based compounds dissolved in water showed that in such solutions the UV absorbance at ~185 nm (activity of the OH groups) can be essentially increased with respect to the bulk water. The spike’s glycans – water long-range interaction, activated due to the dissolved sugar-based compound, creates the glass-like stabilizing hydration layer (like in case of the trehalose) effectively inhibiting the virus – host cell binding. It was shown that the chemical structures of the known compounds with proven inhibition of SARS-CoV-2 entry into host cells agree with our findings. The described approach can be effective against human immunodeficiency viruses, influenza viruses, and possibly other enveloped viruses. <br>

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Section: Far-uv Absorption Spectra Of Ascorbic Acid (Aa)supporting

confidence: 65%

Water-SARS-CoV-2 Interaction-Based Mechanism Inhibiting Virus Attachment to Host Cells

Shalatonin

2020

Preprint

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“…Encapsulation efficiency (EE) is a correlation to them, which tells about the ability of each component, LAA, and TQ to stay in CNPs carrier. Their presence in the sample were detected at specific wavelength; LAA at 244 nm and TQ at 254 nm [ 44 , 45 , 46 ]. EE was measured by a UV–VIS spectrophotometer that detects the amount of light absorbed by a sample at their particular wavelength and displays them in absorbance.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Optimization of Chitosan Nanoparticles Loaded with l-Ascorbic Acid and Thymoquinone

et al. 2018

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The combination of compounds with different classes (hydrophobic and hydrophilic characters) in single chitosan carrier is a challenge due to the hydrophilicity of chitosan. Utilization of l-ascorbic acid (LAA) and thymoquinone (TQ) compounds as effective antioxidants is marred by poor bioavailability and uptake. Nanoparticles (NPs) solved the problem by functioning as a carrier for them because they have high surface areas for more efficient delivery and uptake by cells. This research, therefore, synthesized chitosan NPs (CNPs) containing LAA and TQ, CNP-LAA-TQ via ionic gelation routes as the preparation is non-toxic. They were characterized using electron microscopy, zetasizer, UV–VIS spectrophotometry, and infrared spectroscopy. The optimum CNP-LAA-TQ size produced was 141.5 ± 7.8 nm, with a polydispersity index (PDI) of 0.207 ± 0.013. The encapsulation efficiency of CNP-LAA-TQ was 22.8 ± 3.2% for LAA and 35.6 ± 3.6% for TQ. Combined hydrophilic LAA and hydrophobic TQ proved that a myriad of highly efficacious compounds with poor systemic uptake could be encapsulated together in NP systems to increase their pharmaceutical efficiency, indirectly contributing to the advancement of medical and pharmaceutical sectors.

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“…Previous experiments with cloudy apple juice [4] measured its α254 at 11.54 cm −1 , while in our experiments with the same type of apple juice but with added ascorbic acid set the value of α254 at 152.1 cm −1 (Figure 4). It is known that ascorbic acid has its absorption peak within the deep UV spectra between 243.5 and 265.5 nm [22], therefore it is reasonable to think that the addition of ascorbic acid, which is a common practice in fruit juice production, would represent an immediate interference against UV-C. The approach of a thin-film UV-C reactor has the intention of reducing the depth in which radiation has to travel through and to reach as many cells as possible to inactivate them, being most likely affected the closest ones to the UV-C source.…”

Section: Discussionmentioning

confidence: 99%

Short Wave Ultraviolet Light (UV-C) Effectiveness in the Inactivation of Bacterial Spores Inoculated in Turbid Suspensions and in Cloudy Apple Juice

et al. 2021

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Liquid foods might present interferences in their optical properties that can reduce the effectiveness of short-wave ultraviolet radiation (UV-C) treatments used for sterilization purposes. The effect of turbidity as UV-C interference factor against the inactivation of bacterial spores was analysed by using phosphate-buffered saline solutions (PBS) of different turbidity values (2000, 2500, and 3000 NTU) which were adjusted with the addition of apple fibre. These suspensions were inoculated with spores of Bacillus subtilis and Alicyclobacillus acidoterrestris. While higher UV-C doses increased the inactivation rates of spores, these were reduced when turbidity values increased; a dose of 28.7 J/mL allowed inactivation rates of B. subtilis spores of 3.96 Log in a 2000-NTU suspension compared with 2.81 Log achieved in the 3000-NTU one. Spores of B. subtilis were more UV-C-resistant than A. acidoterrestris. Cloudy apple juice inoculated with A. acidoterrestris spores was processed by UV-C at different doses in a single pass and with recirculation of the matrix through the reactor. Inactivation increased significantly with recirculation, surpassing 5 Log after 125 J/mL compared with 0.13 Log inactivation after a single-pass treatment at the same UV-C dose. UV-C treatments with recirculation affected the optical properties (absorption coefficient at 254 nm and turbidity) of juice and increased browning as UV-C doses became higher.

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Investigation ofL(+)-Ascorbic acid with Raman spectroscopy in visible and UV light

Abstract: Raman spectroscopy investigations of l(+)-ascorbic acid and its mono-and di-deprotonated anions (AH

Cited by 45 publications

References 87 publications

Water-SARS-CoV-2 Interaction-Based Mechanism Inhibiting Virus Attachment to Host Cells

Water-SARS-CoV-2 Interaction-Based Mechanism Inhibiting Virus Attachment to Host Cells

Synthesis and Optimization of Chitosan Nanoparticles Loaded with l-Ascorbic Acid and Thymoquinone

Short Wave Ultraviolet Light (UV-C) Effectiveness in the Inactivation of Bacterial Spores Inoculated in Turbid Suspensions and in Cloudy Apple Juice

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