Investigation of SARS-CoV-2 nucleocapsid protein interaction with a specific antibody by combined spectroscopic ellipsometry and quartz crystal microbalance with dissipation

Plikusienė, Ieva; Mačiulis, Vincentas; Juciute, Silvija; Ramanavičius, Arūnas; Balevičius, Zigmas; Slibinskas, Rimantas; Kučinskaitė-Kodzė, Indrė; Simanavičius, Martynas; Balevičius, Saulius

doi:10.1016/j.jcis.2022.06.119

Cited by 17 publications

(15 citation statements)

References 53 publications

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“…Several substrates have been utilized to track the adsorption kinetics of polymers, , DNA, − and proteins , using QCM-D. In this study, we installed four clean silica QCM-D sensors in the QCM-D measurement chamber.…”

Section: Resultsmentioning

confidence: 99%

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca²⁺ Ions by AFM Force Measurement

Meng,

Xie,

Yan

et al. 2024

ACS Appl. Mater. Interfaces

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Quartz is a key raw material in high-tech fields (such as photovoltaics and semiconductor microelectronics), and the most efficient extraction method of quartz is mineral flotation. Quartz activation plays a crucial role in mineral flotation. However, the mechanism underlying the process remains unclear, and the role of additional metal ions is controversial. In this study, the interaction forces between the quartz surface, the dodecylamine (DDA) cation/sodium oleate (NaOL) anion mixed collectors, and Ca 2+ were analyzed using atomic force microscopy in order to systematically explore the activation process of quartz flotation. The results confirmed that the activation process was initialized from NaOL, which was adsorbed on the surface of a calcium-covered quartz surface. The existence of DDA inhibited the binding of Ca 2+ to NaOL so that more Ca 2+ was adsorbed on the quartz surface to provide the adsorption site for NaOL. Moreover, the best adsorption condition of Ca 2+ + NaOL + DDA mixed solution was analyzed by quartz crystal microbalance with dissipation, and it demonstrated that the most stable chemisorption environment on the quartz surface was at pH 11.0. In these circumstances, Ca 2+ could first adsorb in a point-like manner on the quartz surface, which was then adsorbed with a mixture of NaOL and DDA. This result showed that, at a specific pH, Ca 2+ could encourage the coadsorption of cationic/anionic mixed collectors on quartz. This work provides an important new understanding of the intermolecular interactions that take place during complex mineral flotation processes between chemical additives and mineral surfaces. The methodology used in this study can be easily adapted to different interfacial processes, including water treatment, membrane technology, bioengineering, and oil production.

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Section: Resultsmentioning

confidence: 99%

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca²⁺ Ions by AFM Force Measurement

Meng,

Xie,

Yan

et al. 2024

ACS Appl. Mater. Interfaces

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“…A lower value of K D indicates a higher-affinity interaction. The K D value for the antibody interaction with antigen in solution is usually in the range from 10 −9 to 10 −6 M [ 44 ]. K D and B max were calculated to be 1.00 × 10 −7 ± 2.29 × 10 −9 M and 263.32 ± 2.44 m°, respectively.…”

Section: Resultsmentioning

confidence: 99%

Experimental Evaluation of Quantum Dots and Antibodies Conjugation by Surface Plasmon Resonance Spectroscopy

Popov

Lisytė

Kausaitė-Minkstimienė

et al. 2022

IJMS

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The application of antibody-functionalized quantum dots (QDs) in different areas has been widely described in the literature. However, a standard routine method for obtaining information on the conjugation efficiency of QDs with antibodies in terms of the interaction of the functionalized QDs with a specific antigen is still lacking. Herein, surface plasmon resonance (SPR) spectroscopy is proposed for this purpose. Gold-coated SPR sensor disks were modified with a self-assembled monolayer of 11-mercaptoundecanoic acid, and carbodiimide cross-linker chemistry was used to covalently immobilize the CD44 biomarker on the premodified surface (Au/CD44). Meanwhile, QDs functionalized with amine-derivatized polyethylene glycol (PEG) (QDs-NH2) were chosen for conjugation with antibodies because of their low non-specific adsorption on the Au/CD44 surface. Prior to conjugation, the surface binding capacity (Bmax) and equilibrium dissociation constant (KD) of the specific antibodies against CD44 (anti-CD44) were found to be 263.32 ± 2.44 m° and 1.00 × 10−7 ± 2.29 × 10−9 M, respectively. QDs-NH2 and anti-CD44 were conjugated at their initial molar ratios of 1:3, 1:5, 1:10 and 1:12. SPR measurements showed that the conjugates (QDs-anti-CD44) prepared using 1:10 and 1:12 molar ratios interacted comparably with immobilized CD44 biomarkers. The equilibrium angles in the case of 10- and 12-fold concentrations of anti-CD44 were calculated to be 60.43 ± 4.51 and 61.36 ± 4.40 m°, respectively. This could be explained by the QDs-NH2 and anti-CD44 having a similar surface loading (about four molecules per QDs-NH2) and similar hydrodynamic diameters, which were 46.63 ± 3.86 and 42.42 ± 0.80 nm for the 1:10 and 1:12 ratios, respectively. An initial QDs-NH2: anti-CD44 molar ratio of 1:10 was chosen as being optimal. SPR spectroscopy proved to be the right choice for QDs-anti-CD44 conjugation optimization, and can be used for the evaluation of conjugation efficiency for other nanostructures with various bio-recognition molecules.

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“…Antigen-antibody interaction, nucleic acid hybridization, and changes in reactive oxygen species (ROS) levels have all been major contributors to the generation of analytical signals in recent years. 18 Precision measurement of these signals is achievable through the utilization of diverse techniques and transducers, such as fieldeffect transistors (FETs), surface plasmon resonance (SPR), electrochemical biosensing, [19][20][21] electrochemiluminescence (ECL), lateral flow immunoassay (LFIA), optical biosensing, ellipsometry, [22][23][24] and others. 25 Efforts focused on emphasizing the need for suitable research instruments and providing essential resources can lead to the development of more efficient point-of-care (POC) diagnostics.…”

Section: Introduction: a Demi-decade In Combatting The Sars-cov-2 Out...mentioning

confidence: 99%

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Sadique,

Yadav,

Khan

et al. 2024

Chem. Soc. Rev.

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Investigation of SARS-CoV-2 nucleocapsid protein interaction with a specific antibody by combined spectroscopic ellipsometry and quartz crystal microbalance with dissipation

Cited by 17 publications

References 53 publications

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca²⁺ Ions by AFM Force Measurement

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca²⁺ Ions by AFM Force Measurement

Experimental Evaluation of Quantum Dots and Antibodies Conjugation by Surface Plasmon Resonance Spectroscopy

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

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Investigation of SARS-CoV-2 nucleocapsid protein interaction with a specific antibody by combined spectroscopic ellipsometry and quartz crystal microbalance with dissipation

Cited by 17 publications

References 53 publications

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca2+ Ions by AFM Force Measurement

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca2+ Ions by AFM Force Measurement

Experimental Evaluation of Quantum Dots and Antibodies Conjugation by Surface Plasmon Resonance Spectroscopy

Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Contact Info

Product

Resources

About

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca²⁺ Ions by AFM Force Measurement

Probing the Interaction Mechanism of Sodium Oleate and Dodecyl Amine with Quartz Surfaces in the Presence of Ca²⁺ Ions by AFM Force Measurement