Investigation of round goby viral haemorrhagic septicaemia outbreak in New York

Getchell, Rodman G.; First, Erika J.; Bogdanowicz, Steven M.; Andrés, José Á.; Schulman, Adam T.; Kramer, Jordan J.; Eckerlin, Geofrey E.; Farrell, John M.; Marquis, Hélène

doi:10.1111/jfd.13003

Cited by 5 publications

(6 citation statements)

References 33 publications

(55 reference statements)

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“…A more recent analysis of 11 other VHSV‐IVb whole‐genome positives, including five from the 2017 Cayuga Lake outbreak and four from a 2017 round goby kill at Long Point State Park, NY by Getchell et al. (2019), discerned 379 SNPs, with 123 being nonsynonymous (33.4%), which also is higher than ours for both and might again reflect sampling size or a mutation rate difference. Similar to our results, a Zika Virus study found 16% nonsynonymous changes among 1,030 SNPs across 110 whole genomes (Metsky et al., 2017), close to the proportion we identified here in the partial G ‐gene sequences and across the entire VHSV‐IVb genome.…”

Section: Discussioncontrasting

confidence: 62%

“…Getchell et al. (2019) also reported polymorphisms at single NT positions in some isolates from the Cayuga Lake 2017 outbreak. Here, we found no evidence of multiple haplotypes within a single individual.…”

Section: Discussionmentioning

confidence: 95%

“…Single‐infected gizzard shad ( Dorosoma cepedianum ) individuals were reported from Lake Ontario in 2013 and the St. Lawrence River in 2014 (Getchell et al., 2017). In spring 2017, relatively minor and geographically restricted allopatric outbreaks occurred in Lake St. Clair (M. Faisal and G. Whelan, personal commination, 2017), Cayuga Lake (New York Finger Lakes), and Lake Ontario (Getchell et al., 2019; R. Getchell, personal communication, 2017). There have been no reported subsequent outbreaks.…”

Section: Introductionmentioning

confidence: 99%

“…In 2010, large proportions of round goby tested positive, suggesting its persistent susceptibility and that it comprises a possible vector (Cornwell et al, 2014, 2015; Cornwell, Eckerlin, et al, 2012). The 2017 Cayuga Lake NY outbreak also occurred in round goby (Getchell et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

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Evolutionary trajectory of fishPiscine novirhabdovirus(=Viral Hemorrhagic Septicemia Virus) across its Laurentian Great Lakes history: Spatial and temporal diversification

Stepien

Niner

2020

Ecology and Evolution

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Piscine novirhabdovirus = Viral Hemorrhagic Septicemia Virus (VHSV) first appeared in the Laurentian Great Lakes with large outbreaks from 2005 to 2006, as a new and novel RNA rhabdovirus subgenogroup (IVb) that killed >30 fish species. Interlude periods punctuated smaller more localized outbreaks in 2007, 2010, and 2017, although some fishes tested positive in the intervals. There have not been reports of outbreaks or positives from 2018, 2019, or 2020. Here, we employ a combined population genetics and phylogenetic approach to evaluate spatial and temporal evolutionary trajectory on its G‐gene sequence variation, in comparison with whole‐genome sequences (11,083 bp) from a subset of 44 individual isolates (including 40 newly sequenced ones). Our results show that IVb (N = 184 individual fish isolates) diversified into 36 G‐gene haplotypes from 2003 to 2017, stemming from two originals (“a” and “b”). G‐gene haplotypes “a” and “b” differed by just one synonymous single‐nucleotide polymorphism (SNP) substitution, remained the most abundant until 2011, then disappeared. Group “a” descendants (14 haplotypes) remained most prevalent in the Upper and Central Great Lakes, with eight (51%) having nonsynonymous substitutions. Group “b” descendants primarily have occurred in the Lower Great Lakes, including 22 haplotypes, of which 15 (68%) contained nonsynonymous changes. Evolutionary patterns of the whole‐genome sequences (which had 34 haplotypes among 44 isolates) appear congruent with those from the G‐gene. Virus populations significantly diverged among the Upper, Central, and Lower Great Lakes, diversifying over time. Spatial divergence was apparent in the overall patterns of nucleotide substitutions, while amino acid changes increased temporally. VHSV‐IVb thus significantly differentiated across its less than two decades in the Great Lakes, accompanied by declining outbreaks and virulence. Continuing diversification likely allowed the virus to persist at low levels in resident fish populations, and may facilitate its potential for further and future spread to new habitats and nonacclimated hosts.

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Section: Discussioncontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evolutionary trajectory of fishPiscine novirhabdovirus(=Viral Hemorrhagic Septicemia Virus) across its Laurentian Great Lakes history: Spatial and temporal diversification

Stepien

Niner

2020

Ecology and Evolution

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“…Given the presence of VHSV-IVb throughout the Great Lakes watershed there is tangible risk to all farmed fish in the region [22,23]. VHSV-IVa/b are reported to cause mild to modest disease in rainbow trout (Oncorhynchus mykiss) [24,25], but disease kinetics and mortalities may be comparable to those seen in yellow perch (Perca flavescens) and round gobies (Neogobius melanostomus), which are highly susceptible to this pathogen [26][27][28]. Additional challenge studies show that VHSV-IVb can cause significant morbidity and mortality in rainbow trout [29,30].…”

Section: Introductionmentioning

confidence: 99%

Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types

Gorgoglione

Ringiesn

Pham

et al. 2020

Virol J

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Background: Infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) are highly contagious, pathogenic Novirhabdoviruses affecting fish and are thusly notifiable diseases with the World Organization for Animal Health. This study assessed the relative capacities of IHNV and VHSV genes to modulate host general transcription and explores the abilities of specific IHNV genes to interfere with the interferon pathway in heterogenous teleost cell-lines. Methods: Optimized protocols allowed for efficient transient transfections in EPC, BF-2, RTG-2 and RTgill-W1 cell lines of plasmids encoding IHNV (M genogroup) and VHSV (-IVb genotype) genes, including N, P, M, G and NV. Their impact on general cellular transcription was measured 48 hours post transfection (hpt) with luciferase constructs driven by a modified β-Actin promoter (pCAG). Their modulation of the innate antiviral immune response was characterized 72 hpt, using luciferase constructs measuring rainbow trout Type I IFN or MX-1 promoter augmentation, upon MAVS cotransfection. Results: M was generally confirmed as the strongest constitutive transcriptional suppressor while IHNV P, but not VHSV P, augmented constitutive transcription in fibroblastic cell types. Cell-specific effects were observed for viral G gene, with VHSV G exhibiting suppression of basal transcription in EPC and BF-2 but not in trout cells; while IHNV G was stimulatory in RTG-2, but inhibitory in RTgill-W1. NV consistently stimulated constitutive transcription, with higher augmentation patterns seen in fibroblastic compared to epithelial cells, and for IHNV NV compared to VHSV NV. The innate antiviral immune response, focusing on the IFN pathway, was silenced by IHNV M in all cell lines tested. IHNV N showed a dose-dependent suppression of type I IFN, but with minor effects on MX-1. IHNV P and G played minor IFNinhibitory roles, consistent and dose-dependent only for G in rainbow trout cells. IHNV NV mediated a consistent stimulatory effect on either Type I IFN or MX-1, but much less pronounced in RTgill-W1.

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Eastward Expansion of Round Goby in New York: Assessment of Detection Methods and Current Range

et al. 2021

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The Round Goby Neogobius melanostomus has spread rapidly around the Great Lakes region since its introduction to North America in 1990. In 2014, a specimen was captured in the New York State Canal System west of Utica, prompting concerns that Round Goby would soon reach the ecologically and economically valuable watersheds of Lake Champlain and the Hudson River estuary. The establishment of Round Goby populations elsewhere has been linked to a number of negative ecological consequences, yet methods for monitoring the invasion front of this species remain limited. The objectives of this study were to assess the current distribution of Round Goby in central New York and to determine the most effective methods for monitoring the invasion front. This was achieved by concurrently using benthic trawling, seining, minnow traps, and environmental DNA (eDNA) twice annually from 2016 to 2019 at 12 sites on the canal system between Oneida Lake and the Hudson River. Of the three traditional gear types, benthic trawling was the most effective method and captured Round Goby as far east as Utica by 2019. This finding suggests only minimal eastward expansion of Round Goby occurred between 2014 and 2019. Round Goby DNA was detected in water samples during all surveys in which individuals were captured with trawling, and the estimated concentration of DNA explained 69% of the variability in trawl catch. At multiple study sites, Round Goby DNA was identified during consecutive surveys before Round Goby were first captured with trawling. This suggests that in lotic waters, eDNA has the potential to forecast or serve as a sentinel for the expansion of Round Goby to new locations. Our results demonstrate the importance of using eDNA in a repeated sampling framework and supplementing eDNA sampling with some level of effort with traditional sampling methods.

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Investigation of round goby viral haemorrhagic septicaemia outbreak in New York

Cited by 5 publications

References 33 publications

Evolutionary trajectory of fishPiscine novirhabdovirus(=Viral Hemorrhagic Septicemia Virus) across its Laurentian Great Lakes history: Spatial and temporal diversification

Evolutionary trajectory of fishPiscine novirhabdovirus(=Viral Hemorrhagic Septicemia Virus) across its Laurentian Great Lakes history: Spatial and temporal diversification

Comparative effects of Novirhabdovirus genes on modulating constitutive transcription and innate antiviral responses, in different teleost host cell types

Eastward Expansion of Round Goby in New York: Assessment of Detection Methods and Current Range

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