Investigation of Rotavirus with Various Methods in Children with Acute Gastroenteritis and Determination of Its Molecular Epidemiology in Kayseri Province, Turkey

Abstract

Consequently, it was revealed that the sensitivity of ELISA and immunochromatographic methods, which provide results in a short time and are used in the investigation of rotavirus antigen, was high and their specificity was low; further studies to determine the distribution of G and P genotypes will contribute to establishing strategies for vaccine development for rotavirus in the world.

Prevalence and diversity of rotavirus A genotypes cirulating in Turkey during a 2‐year sentinel surveillance period, 2014‐2016

“…G3P[8] was the second most common genotype (19.6%) overall, as it predominated in Marmara and South‐East Anatolia and was the second most common genotype in the other regions. Previous studies have shown that the G3P[8] genotype persists in Turkey at a rate of 3.5‐9.6% . Consistent with our data, an Australian rotavirus surveillance study reported that G3P[8] was the second most common genotype, detected in 22.8% of all specimens analyzed in 2015 .…”

“…More than one third of infections occur in children younger than 2 years of age, and rates of rotavirus positivity are similar between males and females . Genotyping studies from different provinces over various time periods have indicated that G1‐G4 and G9 are the five most common genotypes and that there is major variation in the frequency of each of these genotypes over time . Although rotavirus vaccination has not yet been included in a universal vaccination program in Turkey, both Rotarix [RV1] and RotaTeq [RV5] are available in Turkish markets, and families who want to vaccinate their children can easily find these vaccines.…”

Molecular Characterization of Rotavirus Strains Causing Gastroenteritis in Children under 5 Years in Cairo, Egypt

“…A total of 198 diarrheal specimens were tested by ELISA for rotavirus antigen and 56 (28.3%) were positive. This finding is within the range (11-76.9%) previously detected in Egypt [22][23][24][25][26][27][28][29] and in other countries .3%), including the Middle East and North Africa [30][31][32][33][34][35][36][37][38][39][40]. The variation in prevalence rates may be attributed to different conditions which may have affected the detection rates.…”

“…For example, in other studies there were differences in the number of tested samples, season of sample collection, and the sampling methods. The occurrence of the group A Rotavirus was higher in the first 12 months of life (36%) than in the other age groups, as was observed in previous studies in developing countries [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41]. This finding may be explained by decline of maternal antibodies with immature immune systems which protect the newborns from pathogens during the first months of life [42].…”

Evaluation of the Ridaquick Rotavirus/Adenovirus Immuno-Chromatographic Assay in Real-Life Situation

“…In contrast, molecular detection of viral targets using PCR result in higher assay sensitivity and specificity, making them the ‘gold standard’ for the detection of viral enteric pathogens [ 9 , 10 ]. Few studies have evaluated the performances of the Ridaquick Rotavirus/Adenovirus Combi ICT (RR/AC ICT) [ 6 , 11 , 12 ]. The aim of the study was to retrospectively assess the sensitivity and specificity of the RR/AC ICT routinely used in point of care settings in comparison with the Fast Track Diagnostics (FTD) Viral Gastroenteritis assay (FTD-GA; Fast Track Diagnostics, Luxembourg), which is a commercial multiplex PCR assay.…”