Investigation of radiolabelled chitosan nanoparticles bearing Cefpodoxime Proxetil, and in vitro antibacterial effect on Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli

Abstract

This study aims to investigate radiolabeled Cefpodoxime Proxetil loaded chitosan (CP-CS) nanoparticles as nuclear imaging infection agent to Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli). The encapsulation efficiency of Cefpodoxime Proxetil was found 82 ± 2%. CP and CP-CS nanoparticles were radiolabeled with Tc-99 m. The radiochemical purity of 99m Tc-CP and 99m Tc-CP-CS nanoparticles were determined by RTLC as 89 ± 3% and 94 ± 2% respectively. In vitro bindings of 99m Tc-CP-CS nanoparticles to S. aureus and E. coli were found higher than 99m Tc-CP bindings.