Investigation of PrPres in dental tissues in variant CJD

Head, Mark W.; Ritchie, Diane; McLoughlin, Victoria; Ironside, James W.

doi:10.1038/sj.bdj.4810536

Cited by 35 publications

(33 citation statements)

References 32 publications

(50 reference statements)

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“…More than half of the thread covered with particles, visible without microscope 23 (10) 2.5 Some part already thickly covered with particles, visible without microscope 7 (3) 3 Totally covered with particles, w50% thickly covered, visible without microscope 8 (4) surgical instruments immediately prior to use in operating theatres (DP, unpublished observations). Further work is required to analyse the nature of the residual contamination, e.g.…”

Section: Discussionmentioning

confidence: 99%

“…3 In vCJD, the abnormal prion form has been observed using immunohistochemistry in the trigeminal ganglion but not in the salivary gland, alveolar nerve, dental pulp or gingival tissue. 4 Previous risk assessments for the potential transmission of vCJD via surgical procedures have stressed the importance of cleaning instruments prior to sterilization. 5 Endodontic files are used to debride and shape the root canal system following removal of the dental pulp.…”

Section: Introductionmentioning

confidence: 99%

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Residual protein levels on reprocessed dental instruments

Smith¹,

Letters²,

Lange

et al. 2005

Journal of Hospital Infection

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Residual protein levels on reprocessed dental instruments

Smith¹,

Letters²,

Lange

et al. 2005

Journal of Hospital Infection

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“…Prion protein accumulation has also been detected in the trigeminal ganglion, raising the possibility that distal branches of the trigeminal nerve may harbour infectivity, although a study of oral tissues from three patients who had died of vCJD has failed to demonstrate the presence of abnormal prion proteins using immunohistochemical techniques. 2 More recent work using very sensitive immunochemical assays has demonstrated the presence of abnormal prions in muscle tissue in cases of sporadic CJD. 3 These more recent findings demonstrate the need for prudence when considering measures to reduce the potential for iatrogenic transmission of human transmissible spongiform encephalopathies (TSEs).…”

Section: Introductionmentioning

confidence: 99%

A study of visual and blood contamination on reprocessed endodontic files from general dental practice

et al. 2005

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Objective This study examined methods used for reprocessing endodontic instruments in general dental practice and determined the degree of residual visual contamination and blood contamination on 250 reprocessed files collected from 25 general dental practices. Materials and methods A questionnaire was administered to 25 general dental practitioners to obtain information on the re-processing of used endodontic files. Ten files which had been used and reprocessed were also collected from each practice. These were examined visually under a dissecting light microscope for residual contamination and then tested for blood deposits using the Kastle-Meyer test. Results Nineteen of the 25 practices used stainless steel hand files. No practitioners used endodontic files as single use devices. Ninety-two per cent of the practitioners discarded and replaced files when they were bent or damaged. Several decontamination methods were reported. The two combinations employed most frequently were manual cleaning and autoclaving or manual cleaning, followed by ultrasonic cleaning and autoclaving. Of the 250 files, 75% showed some degree of visual contamination and seven percent tested positive for residual blood. Blood contaminated files were significantly more heavily contaminated when examined visually. Large variations were found in residual contamination of files collected from practices using the same methods of decontamination. Conclusions While all practitioners re-used endodontic files, the variations in decontamination methods reported indicate a lack of clarity on best practice. This study demonstrates that endodontic files are not reliably decontaminated by methods currently employed in dental practice.

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“…Sur des tissus prélevés chez 5 patients décédés du vMCJ (cerveau, ganglion trigéminal, nerf alvéolaire, pulpe dentaire, gencive, glandes salivaires, langue et amygdales) la présence de l'agent infectieux a été retrouvé seulement dans le cerveau, le ganglion trigéminal et les amygdales [18]. Cependant, ces résultats doivent être considérés avec précaution car le nombre d'échantillons était faible.…”

Section: Discussionunclassified

Risque de transmission des prions lors de la prise en charge d’un patient suspect de maladie de Creutzfeldt-Jakob sporadique

Gourieux

Lemoine

Bornert

et al. 2010

Med Buccale Chir Buccale

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Mots clés : maladie de Creutzfeldt-Jakob / hygiène / prévention Résumé -Les maladies à prions ou encéphalopathies spongiformes subaiguës transmissibles (ESST) sont des maladies neurodégénatives dont l'issue est fatale. Il n'existe aucun test diagnostique fiable ni aucun traitement. Elles représentent de ce fait une préoccupation pour les professionnels de santé, en particulier pour la prévention de leur transmission. Les tissus dentaires, notamment la pulpe dentaire richement innervée et le tissu gingival, sont des tissus définis comme à faible risque d'infectiosité même, si à ce jour, aucun lien d'iatrogénie par la réalisation d'actes bucco-dentaires n'a été établi. La prise en charge d'un patient suspect de maladie de Creutzfeldt-Jakob sporadique dans le service de Médecine et Chirurgie buccales a nécessité la mise en place d'une organisation adaptée pour prévenir toute contamination et pour assurer la protection des professionnels de santé. Elle a été l'occasion de faire une mise au point sur les textes réglementaires et leur évolution. Lors de la prise en charge d'un patient pour la réalisation d'un acte bucco-dentaire, la prévention repose essentiellement sur l'évaluation du risque lié à la transmission de l'agent infectieux des EST et sur le respect scrupuleux des procédures d'hygiène et de stérilisation à appliquer pour l'unit dentaire et les dispositifs médicaux. Key words:Creutzfeldt-Jakob disease / hygiene / prevention Abstract -Focus on prions transmission risk in the care of a patient with suspected sporadic Creutzfeldt-Jakob disease. Prion diseases known as transmissible spongiform encephalopathies (TSEs) are neurodegenerative diseases whose outcome is fatal. There is at present no reliable diagnostic test and no available treatment. They represent therefore major concern for health professionals with regard to prevention of their transmission. The dental tissues, including the richly innervated dental pulp and the gingiva, are tissues considered to have low risk of infectivity although to date no links with dental procedures has been established. The management of one patient with suspected sporadic Creutzfeldt-Jakob disease (CJD) in the department of Oral Surgery necessitated the establishment of a structure adapted to prevent contamination and to protect health professionals. It was an opportunity to refocus on the official guidelines and their evolution. Prevention during dental procedures is essentially based on two factors: risk assessment of transmission of CJD and strict adherence to hygiene procedures and medical devices sterilization.La prévention du risque infectieux au cours de la réalisa-tion des actes de soins en médecine bucco-dentaire fait partie intégrante de la démarche des professionnels de santé. Elle se

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Investigation of PrPres in dental tissues in variant CJD

Cited by 35 publications

References 32 publications

Residual protein levels on reprocessed dental instruments

Residual protein levels on reprocessed dental instruments

A study of visual and blood contamination on reprocessed endodontic files from general dental practice

Risque de transmission des prions lors de la prise en charge d’un patient suspect de maladie de Creutzfeldt-Jakob sporadique

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