Investigation of protein adsorption performance of Ni²⁺‐attached diatomite particles embedded in composite monolithic cryogels

Abstract

As a low-cost natural adsorbent, diatomite (DA) (2 μm) has several advantages including high surface area, chemical reactivity, hydrophilicity and lack of toxicity. In this study, the protein adsorption performance of supermacroporous composite cryogels embedded with Ni(2+)-attached DA particles (Ni(2+)-ADAPs) was investigated. Supermacroporous poly(2-hydroxyethyl methacrylate) (PHEMA)-based monolithic composite cryogel column embedded with Ni(2+)-ADAPs was prepared by radical cryo-copolymerization of 2-hydroxyethyl methacrylate (HEMA) with N,N'-methylene-bis-acrylamide (MBAAm) as cross-linker directly in a plastic syringe for affinity purification of human serum albumin (HSA) both from aqueous solutions and human serum. The chemical composition and surface area of DA was determined by XRF and BET method, respectively. The characterization of composite cryogel was investigated by SEM. The effect of pH, and embedded Ni(2+)-ADAPs amount, initial HSA concentration, temperature and flow rate on adsorption were studied. The maximum amount of HSA adsorption from aqueous solution at pH 8.0 phosphate buffer was very high (485.15 mg/g DA). It was observed that HSA could be repeatedly adsorbed and desorbed to the embedded Ni(2+)-ADAPs in poly(2-hydroxyethyl methacrylate) composite cryogel without significant loss of adsorption capacity. The efficiency of albumin adsorption from human serum before and after albumin adsorption was also investigated with SDS-PAGE analyses.

TRII: A Probabilistic Scoring of Drosophila melanogaster Translation Initiation Sites

“…In particular, the optimized high-confidence sets of annotated translation start sites were used to assess sequence conservation at initiation sites and to compare this conservation with previous descriptions of consensus sequences [23, 24]. Figure 8 shows the nucleotide frequencies and corresponding relative information profiles for an optimized 0-upAUG set consisting of from which the 22 sequences (5%) with lowest TRII scores have been excluded to remove outliers.…”

Immobilization of xylanase enzyme on poly-(HEMA-co-GMA) cryogel

“…The weak side of cryogels is their low adsorption capacity. This is due to the low surface area of the super macropores in the matrix [12][13][14]. Improving the binding capacity in some processes based on cryogels is of great importance [15].…”

Investigation of BSA adsorption performances of metal ion attached mineral particles embedded cryogel discs

“…The boosting of cryogel properties will carry forward its potential usage in various areas [17]. The weak side of cryogels that adsorption capacities of them for proteins is not at intended levels since the low surface area of inter-connected supermacropores within the matrix [23,24,25]. Enhancing the binding performance of cryogels has a great significance in bioseparation operations [26].…”