Investigation of protective effects of selenium and vitamin e against DNA oxidation, membrane damage and alteration of comt metabolism in smoke-exposed male mice ( Mus Musculus, Balb/c)

Abstract

In our study, the protective effects of vitamin E and Se (selenium) against cigarette smoke hazards on second-hand smoker (passive smoker) male mice (Balb/c) were investigated. Serum MDA levels in the smoke-exposed mice were found higher than serum MDA levels of control mice and Se- and vitamin E-treated mice. But, the MDA levels of smoke-exposed plus Se- and vitamin E-treated mice were found lower than MDA levels of smoke-exposed mice at the end of the three and five months. According to these results, application of vitamin E and Se, when given to smoke-exposed mice together, had an additive protective effect against cigarette smoke hazards (p < 0.05). Vitamin E also had protective effect on formation of 8-OHdG in smoke-exposed mice. The serum 8-OHdG amounts of smoke-exposed plus vitamin E-treated mice were found low, but the serum 8-OHdG amounts of smoke-exposed mice were found high. Also 8-OHdG levels in the serum of the smoke-exposed mice were increased which occurs as a result of DNA oxidation (p < 0.05). At the end of the three and five months, COMT (catechol-o-methyl transferase) activity of smoke-exposed mice livers were increased but, vitamin E and/or Se showed a significant protective effect on changing of COMT activity only at the end of the 5 months. Our results showed that MDA levels and 8-OHdG amounts were increased in the serum of smoke-exposed mice. On the other hand, vitamin E and Se had an additive protective effect against increasing MDA level. Also vitamin E had a protective effect against formation of 8-OHdG amounts and COMT activity alterations.

Antioxidant activity and metabolite profile of quercetin in vitamin-E-depleted rats

“…These effects might result from an alteration of the transport of isorhamnetin from tissue(s) into the circulation, a modification of the affinity of COMT for site-specific O-methylation of flavonol hydroxyl groups and/or an alteration in tamarixetin excretion from the liver into bile. An interaction between vitamin E status and COMT activity was recently suggested by a study in which vitamin E plus selenium prevented an increase in COMT activity induced by cigarette smoke in mice [54]. However, the mechanisms by which vitamin E status alters quercetin metabolism remain to be elucidated.…”

Epigenetic Regulation in Particulate Matter-Mediated Cardiopulmonary Toxicities: A Systems Biology Perspective

“…PM suppresses the intracellular levels of SAM, the methyl donor for DNMT via direct oxidation and inactivation of SAM by ROS [89]. In addition, PM up-regulates catechol-O-methyltransferase (COMT) [90], another methyltransferase utilizing SAM, which also degrades catecholamines such as dopamine, epinephrine, and norepinephrine [91]. …”

dl-trans-3,4-Dihydroxy-1-selenolane (DHSred) heals indomethacin-mediated gastric ulcer in mice by modulating arginine metabolism

“…Formulation of effective and affordable antiulcer agents is an important goal in GI pharmacology, as the prescribed anti-ulcer drugs show various side effects and are expensive [3,4]. The trace element, selenium and some organo-selenium compounds can replenish the antioxidative defense, and are being used for treating gastric ulcers and cancers [5][6][7]. Very recently, a water-soluble selenium compound, DL-trans-3,4-dihydroxy-1-selenolane (DHS red ), synthesized by Iwaoka's group [8], could effectively heal the indomethacin (IND)-induced stomach ulceration in mice [9].…”