Investigation of Prolactin Polymorphism in Buffalo Population of Khuzestan-Iran by PCR-RFLP

Tabar, Y. S.; Fayazi, Jamal; Roshanfekr, H.; Mirzadeh, Khalil; Sadr, Ayeh Sadat

doi:10.3923/javaa.2010.284.286

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…The well-known A–G mutation in the codon for amino acid 103 in exon3 of PRL which produces a polymorphic Rsa I site (Lewin et al 1992) in dairy cattle, was not found in the present study, Similar results have been reported in the Khuzestan-Iran buffalo (Tabar et al 2012) and the Murrah Buffalo (Biradar et al 2014), indicating the monomorphic nature of the locus in buffalo species. However, the amount of milk and fat yield of the GG genotypes was higher compared to the AA genotype in PRL -exon3 and the AA genotype was negatively correlated to the milk fat yield in black-and-white cattle (Khatami et al 2005).…”

Section: Resultssupporting

confidence: 84%

An association analysis betweenPRLgenotype and milk production traits in Italian Mediterranean river buffalo

Liang

et al. 2017

Journal of Dairy Research

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This Research Communication describes the association between genetic variation within the prolactin (PRL) gene and the milk production traits of Italian Mediterranean river buffalo (Bufala mediterranea Italiana). High resolution melting (HRM) techniques were developed for genotyping 465 buffaloes. The association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. Single nucleotide polymorphisms (SNPs) were identified at exons 2 and 5 and at introns 1 and 2. All the SNPs were in Hardy-Weinberg equilibrium, and statistical analysis showed that the polymorphism of intron1 was significantly (P < 0·05) associated with milk yield, milk protein content and peak milk yield. The average contribution of the intron1 genotype (r 2 intron1) to total phenotypic variance in milk production traits was 0·09, and the TT genotype showed lower values than CC and CT genotypes. A nonsynonymous SNP was identified in exon 2, which resulted in an amino acid change from arginine to cysteine. Moreover, the polymorphism of exon 2 was associated significantly with milk fat content (P < 0·05), and the buffaloes with TT genotype showed higher total fat content than the buffaloes with CT genotype. These findings provide evidence that polymorphisms of the buffalo PRL gene are associated with milk production traits and PRL can be used as a candidate gene for marker-assisted selection in Italian Mediterranean river buffalo breeding.

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Section: Resultssupporting

confidence: 84%

An association analysis betweenPRLgenotype and milk production traits in Italian Mediterranean river buffalo

Liang

et al. 2017

Journal of Dairy Research

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“…PRL exon 3 digestion with RsaI resulted only in AA genotype and only the A allele for Anatolian water buffalo breed. Similarly, only AA genotype was obtained from the Iranian (Tabar et al, 2010), the Murrah (Biradar et al, 2014), and the Anatolian (Kaplan and Boztepe, 2000) breeds. However, it has been reported that AB and BB genotypes, in addition to AA genotype, were found in Anatolian water buffalo breed (Konca and Akyüz, 2017).…”

Section: Discussionmentioning

confidence: 99%

Investigation of PRL-RsaI and HaeIII gene polymorphisms in Anatolian water buffaloes bred by using PCR-RFLP method

Özşensoy

2018

R. Bras. Zootec.

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The objective of this study was to investigate polymorphisms both in exons 1 and 3 of prolactin (PRL) gene for milk productivity of Anatolian water buffalo breed in Sivas province in Turkey. Blood samples were collected from 129 male and female water buffaloes and DNA was isolated by using phenol/chloroform method. Samples of DNA were amplified and resulting PCR products were digested with RsaI (for exons 1 and 3) and HaeIII (for exon 1). Allelic polymorphisms were determined by separation of fragments obtained from digested PCR products in 3% agarose gel electrophoresis. AA genotype (HaeIII) and BB genotype (RsaI) of exon 1 and only AA genotype (RsaI) of exon 3 were obtained. No polymorphisms were determined in Anatolian water buffalo breed and all loci were found as monomorphic. It can be stated that Anatolian water buffalo has a higher milk and milk fat yields since BB genotype was obtained.

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Investigation of Prolactin Polymorphism in Buffalo Population of Khuzestan-Iran by PCR-RFLP

Cited by 2 publications

References 9 publications

An association analysis betweenPRLgenotype and milk production traits in Italian Mediterranean river buffalo

An association analysis betweenPRLgenotype and milk production traits in Italian Mediterranean river buffalo

Investigation of PRL-RsaI and HaeIII gene polymorphisms in Anatolian water buffaloes bred by using PCR-RFLP method

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