Investigation of prevalence and risk factors for<i>Campylobacter</i>in broiler flocks at slaughter: results from a UK survey

Lawes, J. R.; Vidal, Ana Maria Centola; Clifton-Hadley, F. A.; Sayers, Robin; Rodgers, John; Snow, L. C.; Evans, S. J.; Powell, L. F.

doi:10.1017/s0950268812000982

Cited by 47 publications

(65 citation statements)

References 39 publications

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“…Caecal samples were collected from broiler slaughter batches as part of a 3-year randomized national prevalence survey [13]. Briefly, per slaughter batch, a single caecum was collected for sampling from 10 different broilers at the time of evisceration.…”

Section: Sample and Data Collectionmentioning

confidence: 99%

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Bayesian analysis of culture and PCR methods for detection of Campylobacter spp. in broiler caecal samples

et al. 2014

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The objective of this study was to estimate the sensitivity and specificity of a culture method and a polymerase chain reaction (PCR) method for detection of two Campylobacter species: C. jejuni and C. coli. Data were collected during a 3-year survey of UK broiler flocks, and consisted of parallel sampling of caeca from 436 batches of birds by both PCR and culture. Batches were stratified by season (summer/non-summer) and whether they were the first depopulation of the flock, resulting in four sub-populations. A Bayesian approach in the absence of a gold standard was adopted, and the sensitivity and specificity of the PCR and culture for each Campylobacter subtype was estimated, along with the true C. jejuni and C. coli prevalence in each sub-population. Results indicated that the sensitivity of the culture method was higher than that of PCR in detecting both species when the samples were derived from populations infected with at most one species of Campylobacter. However, from a mixed population, the sensitivity of culture for detecting both C. jejuni or C. coli is reduced while PCR is potentially able to detect both species, although the total probability of correctly identifying at least one species by PCR is similar to that of the culture method.

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Section: Sample and Data Collectionmentioning

confidence: 99%

“…Speciation tests were performed on one single colony per positive batch as described previously [13].…”

Section: Culture Of Pooled Caecal Samplementioning

confidence: 99%

Bayesian analysis of culture and PCR methods for detection of Campylobacter spp. in broiler caecal samples

et al. 2014

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“…C. jejuni infection, unlike C. coli infection, has a marked seasonality, and its occurrence almost doubles in the summertime. This can be explained by a higher contamination of food, especially poultry, with C. jejuni during the hotter period of the year (10). Furthermore, eating habits tend to be different in the summertime, with consumption of more barbecued, undercooked poultry and possibly cross contamination of salads.…”

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Comparison of Characteristics of Patients Infected by Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus

et al. 2014

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A large database of Campylobacter isolates precisely identified at the species level was used to compare patients' characteristics. In a multivariate analysis, Campylobacter coli was found more often in older patients and in patients having traveled abroad and less often in summertime than Campylobacter jejuni. Campylobacter fetus infection occurred in much older patients and in hospitalized patients with a systemic disease.T hermotolerant Campylobacter spp. are recognized as the leading cause of bacterial enteric infections worldwide, while Campylobacter fetus often causes systemic infection.A subset of French clinical laboratories participates in the surveillance of Campylobacter infection, carried out by the National Reference Centre (NRC) for Campylobacters and Helicobacters under the leadership of the Institut de Veille Sanitaire (InVS), sending approximately a quarter of campylobacters isolated in France to the NRC.Our aim was to use this large database (Ͼ22,000 strains) to look for differences in the characteristics of the patients and the circumstances under which these Campylobacter species were isolated.(The results of this study were presented as posters at the European Congress of Clinical Microbiology and Infectious Diseases, London, United Kingdom, 2012, and at the Réunion Interdisciplinaire de Chimiothérapie Anti-infectieuse, Paris, France, November 2012.)Network of laboratories. The laboratories sending strains were not randomly selected but were contacted from the list of laboratories already sending isolated Salmonella species to the corresponding NRC. A survey performed in 2009 showed that equal proportions of all strains isolated came from the different regions, with a few exceptions (InVS-NRC, unpublished data). The methods used by these laboratories to isolate Campylobacter species were fairly uniform, including the use of Karmali medium or Campylosel (bioMérieux, Marcy l'Etoile, France) but no filtration techniques.Identification of campylobacters at the NRC. All of the Campylobacter isolates were identified by phenotypic methods at the genus level. From 2003 to 2009, identification to species level was performed both by standard phenotypic methods and a real-time PCR targeting the gyrA gene (accuracy, 99.9%) to differentiate Campylobacter jejuni and Campylobacter coli (1). In the case of negative results, primers specific for C. fetus were used. If no result was obtained, a PCR specific for Arcobacter species was carried out (2). These PCRs allowed the identification of approximately 99% of the isolates. For the remaining 1%, the 16S rRNA gene was sequenced. In 2010, these methods were replaced by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry identification, which has an accuracy of 99.4% for C. jejuni and 100% for the other Campylobacter species (3).Susceptibility testing was performed by disk diffusion with the same methods and cutoffs from the Comité de l'Antibiogramme de la Société Française de Microbiologie (CA-SFM) that have been used durin...

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“…The prevalence rates of Campylobacter sp. from broiler flocks are 38.1-79.2% in various European countries [6,7].…”

Section: Introductionmentioning

confidence: 99%

Anti Campylobacter Activity of Extracts of Daphne Mucronata and Symplocos Racemosa Against Avian Isolates

Yahya¹,

Kazmi²

2017

Natl. j. health sci.

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Bacterial gastroenteritis mainly caused by Campylobacter sp. is a major health concern in Pakistan. In order to investigate the primary sources of Campylobacter infection, total 81 cloacal swab samples were collected from different avian species in Karachi. Campylobacter strains were isolated, identified and antibiotic susceptibility pattern was studied. All isolates of Campylobacter were sensitive to Gentamicin and Ofloxacin. It was observed that 23% of the C. jejuni positive isolates were resilient to tetracycline, 45% to ampicillin, and 12% to erythromycin. The Campylobacter isolates showed susceptibility to the extracts of two plants namely Daphne Mucronata and Symplocos racemosa. The sensitivity of the isolates towards these extracts can be an alternative to the traditional antibiotics for the treatment of Campylobacter infections.

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Investigation of prevalence and risk factors forCampylobacterin broiler flocks at slaughter: results from a UK survey

Cited by 47 publications

References 39 publications

Bayesian analysis of culture and PCR methods for detection of Campylobacter spp. in broiler caecal samples

Bayesian analysis of culture and PCR methods for detection of Campylobacter spp. in broiler caecal samples

Comparison of Characteristics of Patients Infected by Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus

Anti Campylobacter Activity of Extracts of Daphne Mucronata and Symplocos Racemosa Against Avian Isolates

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