Investigation of plasmid-induced growth defect in Pseudomonas putida

Comparison of Three Xylose Pathways in Pseudomonas putida KT2440 for the Synthesis of Valuable Products

“…Interestingly, no mutations in the xylose utilization genes and other encoding areas of the vectors could be detected. Different studies showed that mutations in the replication initiation protein or in the antibiotic resistance cassette could enhance growth by lowering the plasmid copy number, and subsequently reducing the need of resources for the synthesis of kanamycin resistance (Jakob et al, 2013;Mi et al, 2016). A second possible bottleneck could be the transport of xylonate from the periplasm to the cytoplasm.…”

Pseudomonas mRNA 2.0: Boosting Gene Expression Through Enhanced mRNA Stability and Translational Efficiency

“…Genomic integration is generally preferred over plasmid-based expression when it comes to the stable construction of cell factories. Integrating the pathways into the genome grants higher genetic stability since it avoids common plasmidbased expression issues such as plasmid segregation and copy number variability (Jahn et al, 2014;Lindmeyer et al, 2015), plasmid replication-related growth impairment (Mi et al, 2016), and antibiotic dependency. However, genomic integration possesses specific disadvantages, like generally significantly lower expression levels and a limited number of characterized integration sites (Otto et al, 2019).…”

PseudomonasmRNA 2.0: Boosting Gene Expression Through Enhanced mRNA Stability and Translational Efficiency

“…Genomic integration is generally preferred over plasmid-based expression when it comes to the stable 330 construction of cell factories. Integrating the pathways into the genome grants higher genetic stability 331 since it avoids common plasmid-based expression issues such as plasmid segregation and copy number 332 variability (Jahn et al, 2014;Lindmeyer et al, 2015), plasmid replication-related growth impairment 333 (Mi et al, 2016), and antibiotic dependency. However, genomic integration possesses specific 334 disadvantages like generally significantly lower expression levels and a limited number of 335 characterized integration sites .…”