Investigation of photoacoustic spectroscopy for biomolecular detection

Maswadi, Saher; Glickman, Randolph D.; Barsalou, Norman; Elliott, R.

doi:10.1117/12.674408

Cited by 5 publications

(5 citation statements)

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“…The propagation of the pressure wave produces a local density gradient, which alters the refractive index of the coupling medium (in this case DI water) which is directly proportional to pressure (piezo-optic coefficient dn/dP =

for water [29] ), leading to beam deflection. PBDT has been extensively described in our previous work [17] , [24] , [25] , [26] , [27] . In short, the sensor beam interacts with a spherical shape pressure wave generated from a point source and the interaction length between the probe beam and the pressure waterfront is limited to a small area or just a point of interaction as illustrated in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…In this paper, we introduce a novel OAM design that utilizes an all-optical method, the probe beam deflection technique (PBDT), for ultrasound generation and detection [17] , [24] , [25] , [26] , [27] . A recent review of all-optical OAM methods [28] did not mention the PBDT, which emphasizes the novelty of this method in optoacoustic microscopy.…”

Section: Introductionmentioning

confidence: 99%

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All-optical optoacoustic microscopy based on probe beam deflection technique

Maswadi¹,

Ibey

Roth

et al. 2016

Photoacoustics

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Optoacoustic (OA) microscopy using an all-optical system based on the probe beam deflection technique (PBDT) for detection of laser-induced acoustic signals was investigated as an alternative to conventional piezoelectric transducers. PBDT provides a number of advantages for OA microscopy including (i) efficient coupling of laser excitation energy to the samples being imaged through the probing laser beam, (ii) undistorted coupling of acoustic waves to the detector without the need for separation of the optical and acoustic paths, (iii) high sensitivity and (iv) ultrawide bandwidth. Because of the unimpeded optical path in PBDT, diffraction-limited lateral resolution can be readily achieved. The sensitivity of the current PBDT sensor of 22 μV/Pa and its noise equivalent pressure (NEP) of 11.4 Pa are comparable with these parameters of the optical micro-ring resonator and commercial piezoelectric ultrasonic transducers. Benefits of the present prototype OA microscope were demonstrated by successfully resolving micron-size details in histological sections of cardiac muscle.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

All-optical optoacoustic microscopy based on probe beam deflection technique

Maswadi¹,

Ibey

Roth

et al. 2016

Photoacoustics

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“…10C ) by a motorized stage capable of moving 25 mm in the X-plane and 12 mm in the Y-plane. When a wave generated from the electrodes intersected the probe beam, the variation in the refractive index of the medium caused the probe beam to deflect from its original direction, which appeared as an intensity and/or trajectory change in the output of the position detector 17 18 19 20 21 22 .…”

Section: Methodsmentioning

confidence: 99%

“…We hypothesize that pressure transients created by nsEP exposure 17 are directly linked to the phenomena of nanoporation. We used the probe beam deflection technique (PBDT), an all-optical, non-contact method for detecting pressure transients generated in gaseous and liquid environments to characterize the pressure transients generated by typical nsEP expsoures 18 19 20 21 22 . With PBDT, the propagation of a pressure transient causes a change in the refractive index of the medium through which a probe beam travels, resulting in a deflection of that beam.…”

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confidence: 99%

Characterization of Pressure Transients Generated by Nanosecond Electrical Pulse (nsEP) Exposure

Roth

Barnes

Ibey

et al. 2015

Sci Rep

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The mechanism(s) responsible for the breakdown (nanoporation) of cell plasma membranes after nanosecond pulse (nsEP) exposure remains poorly understood. Current theories focus exclusively on the electrical field, citing electrostriction, water dipole alignment and/or electrodeformation as the primary mechanisms for pore formation. However, the delivery of a high-voltage nsEP to cells by tungsten electrodes creates a multitude of biophysical phenomena, including electrohydraulic cavitation, electrochemical interactions, thermoelastic expansion, and others. To date, very limited research has investigated non-electric phenomena occurring during nsEP exposures and their potential effect on cell nanoporation. Of primary interest is the production of acoustic shock waves during nsEP exposure, as it is known that acoustic shock waves can cause membrane poration (sonoporation). Based on these observations, our group characterized the acoustic pressure transients generated by nsEP and determined if such transients played any role in nanoporation. In this paper, we show that nsEP exposures, equivalent to those used in cellular studies, are capable of generating high-frequency (2.5 MHz), high-intensity (>13 kPa) pressure transients. Using confocal microscopy to measure cell uptake of YO-PRO®-1 (indicator of nanoporation of the plasma membrane) and changing the electrode geometry, we determined that acoustic waves alone are not responsible for poration of the membrane.

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“…Photoacoustic spectroscopy (PAS) has been investigated as a non-invasive technique to ascertain the correct dosage of a therapeutic agent used to treat the eye. 2 By using this technique, it may be possible to analyze drug penetration in animals or humans without sacrificing tissue or taking samples from patients.…”

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confidence: 99%

Non-invasive detection of pharmacological drugs in the eye

Maswadi¹

2006

SPIE Newsroom

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Investigation of photoacoustic spectroscopy for biomolecular detection

Cited by 5 publications

References 0 publications

All-optical optoacoustic microscopy based on probe beam deflection technique

All-optical optoacoustic microscopy based on probe beam deflection technique

Characterization of Pressure Transients Generated by Nanosecond Electrical Pulse (nsEP) Exposure

Non-invasive detection of pharmacological drugs in the eye

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