Abstract:Diabetes Mellitus is a global health problem that leads to macro-and microvascular diseases associated with hyperglycemia. Phytotherapy is one of the alternative ways to cope with this type of disease. The genus Ornithogalum is consumed as a wild edible plant and traditionally used for ailments. This study aims to investigate the phenolic composition using High-Performance Liquid Chromatography as well as antioxidant and antidiabetic effects using spectrophotometric method of Ornithogalum lanceolatum L. aerial… Show more
“…In the literature, there are studies investigating the phytochemical content of Ornithogalum species. Temiz 26 carried out the phytochemical analysis of Ornithogalum lanceolatum extract distributed in Mersin (Turkey) by HPLC and reported that especially the leaf parts contain intense amounts of procateuic acid, rutin, quercetin and gallic acid. Figure 3 LC–MS/MS chromatograms of O. umbellatum extracts.…”
The plants that we use as food in our daily diet and as risk preventers against many diseases have many biological and pharmacological activities. The heat treatments applied during the cooking of the plants cause changes in the phytochemical content and bioactivity. In this study, the phytochemical fingerprint and biological activities of raw and heat-treated extracts of Ornithogalum umbellatum L., which is widely consumed in the Black Sea region, were investigated. The bulb and leaf parts of the plant consumed as food were dried in an oven at 35 °C and then ground into powder. For heat treatment, the plant was boiled at 100 °C for 20 min. Differences in phytochemical contents of raw and heat-treated extracts were determined by ICP-MS, LC–MS/MS, and FTIR analysis. Biological activity was investigated with antiradical, antimicrobial, antimutagenic and antiproliferative activity tests. In this way, the effect of heat treatment on both the phytochemical content and biological activity of the O. umbellatum extract was determined. Gallic acid, procateuic acid and caffeic acid were found as the main compounds in the O. umbellatum extract, while the presence of procateuic aldehyde, vanillin and kaempferol in minor proportions was determined. There was a significant decrease in phenolic compound levels after heat treatment and gallic acid content decreased by 92.6%, procateuic acid content by 90% and caffeic acid content by 84.8%. Significant differences were detected in macro and micro element levels after heat treatment in ICP-MS results. While Cd, Ba and Zn levels of the raw extract increased; Na, Mg, K, Fe, U, Co levels decreased significantly. In FTIR spectrum, shifts and disappearances were observed in some of the vibrations and the emergence of new vibrations was also determined after heat treatment. Raw extract exhibited strong scavenging activity against H2O2 and DPPH and had a broad spectrum antimicrobial property. As a result of heat application, regressions were detected in antiradicalic, antibacterial and antifungal activities. Antimutagenic and antiproliferative activities were determined by the Allium test and a significant decrease in both activities and loss of activity against some chromosomal abnormalities were determined after heat treatment. While the antiproliferative activity of the raw extract was 20%, the activity of the heat-treated extract decreased to 7.6%. The raw extract showed the strongest antimutagenic effect with 69.8% against the unequal distribution of chromatin. Similarly, the antimutagenic activity of the extract, which reduced the bridges by 56.1%, decreased to 0.74% after heat treatment and almost lost its antimutagenic activity. The biological activities of raw O. umbellatum are closely related to the major compounds it contains, and the decrease in the levels of these compounds with the effect of heat was reflected in the activity. Studies investigating the phytochemical contents of plants are very important and the studies investigating biological activities related to phytochemical content are more remarkable. In this study, the phytochemical fingerprint of O. umbellatum was determined, its biological activities were related to the compounds it contained, and the biological activity was found to be heat sensitive.
“…In the literature, there are studies investigating the phytochemical content of Ornithogalum species. Temiz 26 carried out the phytochemical analysis of Ornithogalum lanceolatum extract distributed in Mersin (Turkey) by HPLC and reported that especially the leaf parts contain intense amounts of procateuic acid, rutin, quercetin and gallic acid. Figure 3 LC–MS/MS chromatograms of O. umbellatum extracts.…”
The plants that we use as food in our daily diet and as risk preventers against many diseases have many biological and pharmacological activities. The heat treatments applied during the cooking of the plants cause changes in the phytochemical content and bioactivity. In this study, the phytochemical fingerprint and biological activities of raw and heat-treated extracts of Ornithogalum umbellatum L., which is widely consumed in the Black Sea region, were investigated. The bulb and leaf parts of the plant consumed as food were dried in an oven at 35 °C and then ground into powder. For heat treatment, the plant was boiled at 100 °C for 20 min. Differences in phytochemical contents of raw and heat-treated extracts were determined by ICP-MS, LC–MS/MS, and FTIR analysis. Biological activity was investigated with antiradical, antimicrobial, antimutagenic and antiproliferative activity tests. In this way, the effect of heat treatment on both the phytochemical content and biological activity of the O. umbellatum extract was determined. Gallic acid, procateuic acid and caffeic acid were found as the main compounds in the O. umbellatum extract, while the presence of procateuic aldehyde, vanillin and kaempferol in minor proportions was determined. There was a significant decrease in phenolic compound levels after heat treatment and gallic acid content decreased by 92.6%, procateuic acid content by 90% and caffeic acid content by 84.8%. Significant differences were detected in macro and micro element levels after heat treatment in ICP-MS results. While Cd, Ba and Zn levels of the raw extract increased; Na, Mg, K, Fe, U, Co levels decreased significantly. In FTIR spectrum, shifts and disappearances were observed in some of the vibrations and the emergence of new vibrations was also determined after heat treatment. Raw extract exhibited strong scavenging activity against H2O2 and DPPH and had a broad spectrum antimicrobial property. As a result of heat application, regressions were detected in antiradicalic, antibacterial and antifungal activities. Antimutagenic and antiproliferative activities were determined by the Allium test and a significant decrease in both activities and loss of activity against some chromosomal abnormalities were determined after heat treatment. While the antiproliferative activity of the raw extract was 20%, the activity of the heat-treated extract decreased to 7.6%. The raw extract showed the strongest antimutagenic effect with 69.8% against the unequal distribution of chromatin. Similarly, the antimutagenic activity of the extract, which reduced the bridges by 56.1%, decreased to 0.74% after heat treatment and almost lost its antimutagenic activity. The biological activities of raw O. umbellatum are closely related to the major compounds it contains, and the decrease in the levels of these compounds with the effect of heat was reflected in the activity. Studies investigating the phytochemical contents of plants are very important and the studies investigating biological activities related to phytochemical content are more remarkable. In this study, the phytochemical fingerprint of O. umbellatum was determined, its biological activities were related to the compounds it contained, and the biological activity was found to be heat sensitive.
“…In the species O. armeniacum and O. lanceolatum the major phenolic components found were (+)-catechin and gallic acids [ 29 ]. In a latter study [ 14 ], it was found that in ethanolic extracts of O. lanceolatum protocatechuic acid was the main compound in both plant parts (aerial and bulbs) and that rutin, quercetin, and p -coumaric acid were the most predominant phenolic compounds in the aerial parts. Concerning the species O. narbonense , the major phenolic compounds were benzoic acid, p -hydroxybenzoic acid, chlorogenic acid, rutin, and caffeic acid [ 12 ].…”
Section: Resultsmentioning
confidence: 99%
“…There are previous studies regarding the chemical profile and antioxidant activity of Ornithogalum , to which C. reverchonii was considered to belong until 2001 [ 11 ]. For instance, the phenolic profile composition and bioactivity of O. sigmoideum , O. orthophyllum , O. oligophyllum , O. lancelatum, and O. narbonense have been reported [ 12 , 13 , 14 ]. However, this work is the first report in the literature studying the phytochemical composition and biological activity of the genus Cathissa .…”
Cathissa reverchonii (formerly Ornithogalum reverchonii) is a threatened species, constituting an endemism present in the south of Spain and northern Morocco. In Spain, it is only found in two disjoint populations in the region of Andalusia. The determination of its chemical composition and the influence that environmental factors have on it can contribute significantly to the development of appropriate protection and conservation plans. However, there are no previous reports about this species to date. Consequently, this research aimed to study the phenolic composition and antioxidant activity of C. reverchonii and to assess the influence of environmental factors on the phenolic profile and bioactivity. The vegetal material was collected in seven places inhabited by the two separate populations in Spain. The phenolic composition of methanolic extracts of the species was determined by HPLC-ESI-Q-TOF-MS, and the antioxidant activity was assessed by DPPH and ABTS assays. Fifteen compounds were characterized in the extracts of the aerial parts of C. reverchonii, revealing differences in the phytochemical profile between both populations analyzed, mainly in the saponin fraction. The main phenolics were flavone di-C-glucoside (lucenin-2), followed by a quercetin-di-C-glucoside. The composition of the extracts of C. reverchonii and their radical scavenging power were compared with those of other species of the genus Ornithogalum L., revealing significant differences between the latter and the genus Cathissa.
ÖzDiabetes Mellitus (Şeker hastalığı), yüksek kan glukozu (hiperglisemi) ile karakterize edilen ve tüm dünyada en çok muzdarip olunan hastalıklar arasındadır. Hiperglisemi oksidatif stresin oluşum mekanizmalarını tetikleyerek pek çok hastalığın patogenezinde rol oynar. Fitoterapi bu hastalıklar ile başa çıkmada alternatif yollardan biridir. Tıbbi, aromatik ve yabani yenilebilir bitkiler içerdikleri fitokimyasallar sayesinde nutrasötik olarak çeşitli hastalıklara karşı koruyucu etki göstermelerinin yanı sıra besleyici ve diyetetik özelliklere sahiptirler. Sisymbrium türleri yabani yenilebilir bitkiler arasında olup geleneksel olarak çeşitli hastalıkların tedavisinde kullanılmaktadır. Bu çalışmada, Sisymbrium altissimum yapraklarının fenolik bileşiminin yanı sıra antioksidan kapasitesinin ve antidiyabetik özelliklerinin araştırılması amaçlanmıştır. Bu maksatla S. altissimum yaprak ekstraktında (SAE) yüksek performanslı sıvı kromatografisi (HPLC) ile fenolik bileşen tespiti yapıldı. Ayrıca, antioksidan kapasitesini belirlemek amacıyla toplam fenolik ve toplam flavonoid madde tayini ile 2,2-difenil-1-pikrilhidrazil (DPPH) ve 2,2′-Azino-bis (3-etilbenzotiazolin-6-sülfonik asit) (ABTS) serbest radikal temizleme aktivite analizleri gerçekleştirildi. Yaprakların antidiyabetik etkisi, α-amilaz ve α-glukozidaz inhibisyon aktivite tayini ile ölçüldü. Elde edilen sonuçlarda, SAE'nin önemli düzeyde antioksidan aktivite gösterdiği belirlendi. Yapraklarda en çok bulunan fenolik bileşenin rutin olduğu ayrıca, kuersetin ve protokateşuik asidin ise yapraklardaki diğer baskın fenolik bileşenler olduğu saptandı. Diğer taraftan yaprakların α-amilaz ve α-glukozidaz inhibisyon aktivitelerinin akarboz ile karşılaştırıldığında daha düşük olduğu tespit edildi. Sonuç olarak, S. altissimum'um önemli fenolik bileşen içeriğiyle antioksidan durumu güçlendirebileceği ve ayrıca kısmi α-amilaz ve α-glukozidaz inhibisyon aktiviteleri sayesinde nutrasötik olarak yemek sonrası hiperglisemiyi önleyebileceği sonucuna varılabilir.
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