Investigation of phenazine derivatives interaction with DNA by polarized fluorescence method

Blagoi, Y.-U. P.; Zozulya, Victor; Voloshin, Igor; Makitruk, V. L.; Shalamay, A. S.; Shcherbakova, A.S.

doi:10.7124/bc.000462

Cited by 8 publications

(4 citation statements)

References 9 publications

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“…And a moderate cytotoxic impact on MCF7 cells, with an IC50 value of 100μg/mL [34]. The cytotoxic effect of AuNPs is the result of the active physicochemical interaction of gold atoms with the functional groups of intracellular proteins as well as with the nitrogen bases and phosphate groups in DNA, causing cell damage and inhibiting protein synthesis [35][36][37]. M. E. Selim.…”

Section: Ic50 Valuementioning

confidence: 99%

Gold Nanoparticles Induction Using Kombucha SCOBY Disc

Aboul-Nasr

Mohamed

2023

Sohag Journal of Sciences

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SCOBY disc is a unique structure of bacteria and fungi. It was known in the mid-Seventies in Egypt as "Tea fish." To the author's knowledge, no reports since then were published to use them in producing nanoparticles worldwide. The addition of 1mM of (HAuCl4.3H2O) solution into the cell-free extract (CFE) of SCOBY disc grew on black tea medium, resulted in gold nanoparticles AuNPs at 25ºC (Au 3+ →Au 0 ) momentarily. The surface plasmon resonance peaks of the gold colloidal solution produced showed an absorption peak at 530 nm. (TEM) exhibited AuNPs in spherical and triangle shapes (12 to 35 nm). X-ray diffraction reflected four distinct peaks at 2θ = 38.1°, 44.3°, 64.5°, and 77.7°. All four peaks corresponded to standard Bragg reflections ( 111), ( 200), ( 220), and (311) of face center cubic. (FTIR) analysis showed the presence of two amide groups in 1721 & 1033 respectively, which appeared to be responsible for the stability of gold nanoparticles. The mean particle size and polydispersity index (PDI) of AuNPs induced by SCOBY were 61.23 ± 0.416 with a negative charge -10.2 ± 1.73 mV that cause electrostatic repulsion among the nanoparticles and good colloidal stability. Moreover, the effect of AuNPs on inducing apoptosis in liver cancer cells HepG2. 3-(4,5dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) assay showed that AuNPs produced had selective toxic effects to HepG2 cells with IC-50 value 176 µM/mL compared to control cells. These results suggested the use of those green synthesized AuNPs in cancer treatment and further studies are recommended.

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Section: Ic50 Valuementioning

confidence: 99%

Gold Nanoparticles Induction Using Kombucha SCOBY Disc

Aboul-Nasr

Mohamed

2023

Sohag Journal of Sciences

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“…Методами поляризованной флюоресценции и абсорбционной спектроскопии было изучено взаимодействие производных феназина, относящихся к гликозидам и четвертичным солям, с нативной ДНК [53,54] и синтетическими одно-и двухнитевыми полинуклеотидами poly(A), poly(G), poly(A,G), poly(dA)⋅poly(dT), poly(A)⋅poly(U), poly(dG)⋅poly(dC), poly(G)⋅poly(C) [54]. Определены спектроскопические характеристики свободных красителей и их комплексов с нуклеиновыми кислотами, константы связывания с ДНК и полинуклеотидами при низкой ионной силе растворов и условиях, близких к физиологическим.…”

Section: взаимодействие феназиновых производных с днк и синтетическими полинуклеотидами различного состава и структурной организацииunclassified

Biophysical investigations of molecular mechanisms of chemotherapeutic agents action. 1. Chemotherapeutic and antiviral agents (Review)

2019

Biophysical Bulletin

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Background: Determination of molecular mechanisms of action of drugs forms a scientific basis for the directed search of efficient medications. Assumed pathways of interactions of chemotherapeutic drugs which affect infectious agents and malignant neoplasm with their potential molecular targets require direct evidences at the molecular level. Such evidences can be obtained by means of molecular biophysics which possesses an arsenal of new powerful physical techniques for studying the intermolecular interactions of biomolecules and pharmaceutical agents. Objectives: The aim of this review is the generalization of the results of long standing investigations on the molecular mechanisms of action of chemotherapeutic agents performed in the biophysical departments of B. Verkin Institute for Low Temperature Physics and Engineering (ILTPE) of the NAS of Ukraine. The first part of the review is devoted to anticancer and antiviral agents targeted presumably at nucleic acids. Materials and methods: Mass spectrometric studies of molecules of thermally unstable drugs and their complexes with biomolecules have been advanced significantly due to the development of soft ionization/desorption techniques; the researchers of ILTPE have made noticeable contribution to this field. The methods of molecular spectroscopy and computer modeling by means of quantum chemistry were applied in the combined investigations. Results: The objects of study were the systems composed of chemotherapeutic drugs – thiophosphamide, phenazine derivatives and phenazine-modified antigene/antisense oligonucleotides, quaternary compounds, tilorone – and their molecular targets – DNA, oligo- and polynucleotides and nucleic acids components. The mechanisms of action of these drugs established at the model molecular level consisted in the specific and nonspecific noncovalent or covalent interactions of the drugs’ molecules with nucleic acids and their components and in the formation of stable drug-target complexes. Conclusions: The experience of investigations conducted during several decades at the ILTPE has demonstrated the efficiency of the application of the methods and approaches of molecular biophysics to establishing of molecular mechanisms of drugs action. The basic results obtained are of practical importance for the further development of new efficient pharmaceuticals.

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“…The interaction between NA and phenazine natural or synthetic origin are exploring a long time, in [11][12][13]. However, the effects of dissolved gases on such interactions have traditionally not take into account.…”

Section: The Influence Dissolved Gases On the Interactionmentioning

confidence: 99%

“…So, researchers, which studied the interaction between NA and aromatic dyes, used solutions with different pH (from 5.5 to 8) and ionic strengths. In particular, were used: 0,2 M acetate buffer, рН 5, in [4], MES-buffers of different concentrations, рН 6,2, in [5][6], 0,01 М phosphate buffer, рН 7, in [7][8], 0,1 М deuterated phosphate buffer, pD 6,6, in [9], 0,1 мМ Tris-HCl-buffer, pH 8, in [10], 1 mM acetate buffer, рН 6, 4 -6 %-ethanol-containing, in [11], 1 mM acetate buffer, рН 7, in [12], 1 mM Na-cacodylate buffer, рН 7, 0,5 mМ Na2EDTA containing, in [13], etc.…”

Section: Introductionmentioning

confidence: 99%

Nucleic Acids in Atypical Conditions

Pivovarenko¹

2015

EJB

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Abstract:Here we describe some properties of nucleic acids, which observed in 'atypical" experiments. So, we show that physical and chemical properties of nucleic acids depend on dissolved gases. We show particularly that thermal hyperchromism of DNA depends on dissolved oxygen and (or) air. It has been showed most importantly the thermal hyperchromism does not observe for DNA degassed solutions. Here we also demonstrate the difference between some chemical properties of nucleic acids in degassed solutions and in solutions saturated with hydrogen or oxygen. We illustrate too the difference between physical and chemical properties of nucleic acids in the homogenous and gradient-containing solutions; the last case we offer as approximation of the conditions, in which the nucleic acids exist in vivo.

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Investigation of phenazine derivatives interaction with DNA by polarized fluorescence method

Cited by 8 publications

References 9 publications

Gold Nanoparticles Induction Using Kombucha SCOBY Disc

Gold Nanoparticles Induction Using Kombucha SCOBY Disc

Biophysical investigations of molecular mechanisms of chemotherapeutic agents action. 1. Chemotherapeutic and antiviral agents (Review)

Nucleic Acids in Atypical Conditions

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