Investigation of pesticide exposure by genotoxicological, biochemical, genetic polymorphic and in silico analysis

Oliveira, Arielly Furtado Bento de; Souza, Melissa Rosa de; Benedetti, Danieli; Scotti, Amanda Souza; Piazza, Luma Smidt; Garcia, Ana Letícia Hilário; Dias, Johnny Ferraz; Niekraszewicz, Liana Appel Boufleur; Duarte, Anaí; Bauer, D.V.; Amaral, L.; Branco, Carmen Lucia Bassi; Reis, Érica de Melo; Silva, Fernanda Rabaioli da; Silva, Juliana da

doi:10.1016/j.ecoenv.2019.04.023

Cited by 30 publications

(30 citation statements)

References 55 publications

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“…This habit may increase the health risk associated with exposure to pesticides. Therefore, this practice's impact has been widely explored ( Paiva et al, 2011 ; Pedlowski et al, 2012 ; Hernández et al, 2017 ; Bernieri et al, 2019 ; de Oliveira et al, 2019 , Oliveira et al, 2019 ; Marcelino et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Another important issue is individual susceptibility that influences physiological responses to pesticide exposure. Therefore, it is essential to identify genotypes that determine the modulation of the proteins involved in the metabolization, detoxification, and DNA repair, influencing the heterogeneity of responses to pesticides ( de Oliveira et al, 2019 , Oliveira et al, 2019 ). Regarding susceptibility biomarkers, only TNF-α rs361525 polymorphism showed a significant difference in genotype distributions between exposed and control groups (p = 0.002).…”

Section: Discussionmentioning

confidence: 99%

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Multi-biomarker responses to pesticides in an agricultural population from Central Brazil

Ramos

Pedroso

Godoy

et al. 2021

Science of The Total Environment

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We evaluated farmworkers exposed to pesticides and individuals with no history of occupational exposure to pesticides. It was performed the comet assay to evaluate DNA damage. The immunophenotyping of TCD4 + lymphocyte subpopulations in peripheral blood was performed by flow cytometry. The single nucleotide polymorphisms (SNPs) in PON1, XRCC1, IL6, IL6R, TNF-α, and MIR137 genes were evaluated by real-time PCR. The exposed group was composed mostly by males (69.44%), with direct exposure to pesticides (56%) and with an average age range of 46 ± 13.89 years, being that 58.3% of farmworkers directly exposed to pesticides and reported the full use of personal protective equipment (PPE). DNA damage was greater in the exposed group (p < 0.05), reinforced by the use of PPE to denote a lower degree of DNA damage (p = 0.002). In this context, in the exposed group, we demonstrated that the use of PPE, age, gender and intoxication events were the variables that most contributed to increase DNA damage (p < 0.0001). Besides, the exposed group showed a significant increase in the subpopulations of T lymphocytes CD3 + CD4 + (p < 0.05) and CD3 + CD4 + CD25 + (p < 0.0001) and a significant decrease in CD3 + CD4 + CD25 - FOXP3 + (p < 0.05). SNPs in the TNF-α (rs361525) gene presented a difference in the genotype distribution between the groups (p = 0.002). The genotype distribution of TNF-α (rs361525) was also positively correlated with the DNA damage of the exposed group (r = 0.19; p = 0.01), demonstrating a higher risk of DNA damage in the farmworkers presenting the A mutated allele. Our findings demonstrate that pesticides can exert various deleterious effects on human health by damaging the DNA as well as by influencing the immune system in the case of both direct or indirect exposure and these issues are associated to age, gender, intoxication and the nonuse of PPE.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Multi-biomarker responses to pesticides in an agricultural population from Central Brazil

Ramos

Pedroso

Godoy

et al. 2021

Science of The Total Environment

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“…DNA repair genes. Pesticides can directly affect DNA of cells in exposed people, or they can do so indirectly through the production of ROS (Reactive Oxygen Species) that damage the genetic material due to mutagenic lesions or single or doublestranded DNA breaks [11][12][13]. The DNA repair pathways can be affected by different phenotypes of essential genes involved in these pathways.…”

Section: State Of Knowledgementioning

confidence: 99%

“…The DNA repair pathways can be affected by different phenotypes of essential genes involved in these pathways. Base excision repair (BER) is one of the pathways involved in DNA single-strand break repair through the XRCC1 (X-Ray Repair Cross-Complementing Group 1) gene [11,14]. Other affected pathways are the repair of oxidative damage to guanine (8-oxoguanine) by the OGG1 (8-Oxoguanine DNA Glycosylase) gene [4,10,15]; and the non-homologous end-joint mechanism (NHEJ) that is responsible for the repair of double-stranded DNA breaks, by XRCC4 (X-Ray Repair Cross-Complementing Group 4) repair gene.…”

Section: State Of Knowledgementioning

confidence: 99%

“…XRCC1 is essential in the assembly of the BER pathway because it interacts and coordinates other major components such as polyADP-ribose polymerase-1 enzyme, DNA polymerase H, and DNA ligase III, which are all involved in the repair of single-stranded DNA breaks induced by ROS (Fig. 1B) [11][12][20][21][22]. The XRCC1 gene is located on chromosome 19q13.2 with a length of 33 kb, and comprises 17 exons and 16 introns [12,14,19].…”

Section: State Of Knowledgementioning

confidence: 99%

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Genetic profile for the detection of susceptibility to poisoning by exposure to pesticides

Galvez¹,

Salazar-Flores²,

Sanchez³

et al. 2021

Ann Agric Environ Med.

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The Incidence of the XRCC1 rs25487 and PON1 rs662 Polymorphisms in a Population from Central Brazil: Patterns in an Area with a High Level of Agricultural Activity

et al. 2023

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In Brazil, high levels of agricultural activity are re ected in the consumption of enormous amounts of pesticide. The production of grain in Brazil has been estimated to reach 289.8 million tons in the 2022 harvest, an increase of 14.7% in comparison with 2021, with an even greater increase being predicted for Goiás, one of the country's principal grain producing states. These advances are likely associated with a progressive increase in the occupational exposure of rural workers to pesticides. The PON1 gene activates the enzymes involved in phase I of liver detoxi cation. The rs662 variant of this gene is known to modify the activity of the PON1 enzyme, which can increase the susceptibility of an individual to pesticides, leading to more severe symptoms or the development of diseases. The present study describes the frequencies of the rs25487 (of the XRCC gene) and rs662 variants and their haplotypes in a sample population from Goiás, Brazil, and compares the frequencies with other populations from around the world to verify the variation in the distribution of these Single Nucleotide Polymorphisms (SNPs). The study analyzed samples taken from 494 unrelated individuals resident in the state of Goiás. The A allele of the rs25487 variant had a frequency of 26% in the Goiás study population, and the modi ed rs662 G allele had a frequency of 42.8%. Four haplotypes were recorded for the rs25487 (G > A) and rs662 (A > G) markers, with a frequency of 11.9% being recorded for the A/G haplotype (both modi ed alleles), 30.8% for the G/G haplotype, 14.3% for the A/A haplotype, and 42.8% for the G/A haplotype (both wild-type alleles). In conclusion, we demonstrated the distribution of important SNPs associated with pesticide exposure in an area that presents a high level of agricultural activity.

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Investigation of pesticide exposure by genotoxicological, biochemical, genetic polymorphic and in silico analysis

Cited by 30 publications

References 55 publications

Multi-biomarker responses to pesticides in an agricultural population from Central Brazil

Multi-biomarker responses to pesticides in an agricultural population from Central Brazil

Genetic profile for the detection of susceptibility to poisoning by exposure to pesticides

The Incidence of the XRCC1 rs25487 and PON1 rs662 Polymorphisms in a Population from Central Brazil: Patterns in an Area with a High Level of Agricultural Activity

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