Investigation of Pathogenic <i>Escherichia coli</i> Contamination of Vegetables Distributed in a Korean Agricultural Wholesale Market

Oh, Sewon; Han, Su Jin; Gong, Young-Woo; Nam, Hwa-Jung; Kim, Kyung-Ae; Kim, Nam Yee; Kim, Kyung-Seon; Lee, Dong Gun; Shin, Hyunkyoung; Lee, Hye-Bin; Kwon, Mun-Ju; Chae, Joon‐Seok

doi:10.1089/fpd.2021.0027

Cited by 3 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the detection of pathogenic bacteria in wastewater may be valuable for monitoring systems. In a previous study, researchers detected pathogenic E. coli that causes food poisoning in vegetables (water parsley 28.6%, mugwort 28.6%, and sweet potato stem 27.3%), farm soil (36.7%), manure (26.9%), and livestock manure (90.6%) in similar regions assessed in our study [ 35 ]. Farm water used for growing vegetables can be acquired from nearby rivers comprising E. coli .…”

Section: Discussionsupporting

confidence: 68%

“…Particularly, unlike the study conducted in Sweden that reported on animal-derived hepatitis E virus in wastewater [ 32 ], we did not detected hepatitis E virus. In Korea, local community wastewater treatment facilities do not process separately treated livestock wastewater [ 35 ]. The wastewater pipe is designed to pass underground, which prevents contamination with the feces of wild animals.…”

Section: Discussionmentioning

confidence: 99%

“…The reuse of treated wastewater or the flow of wastewater into the nearby sea may become a potential source of Vibrio in the summer. Therefore, monitoring the detection frequency, the concentration of pathogenic microorganisms in local wastewater, and the destination of effluent may be used to predict bacterial food poisoning and public health [ 35 38 ]. As resistance of gut bacteria increases in the community [ 39 ], this importance is likely to increase.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Wastewater Knows Pathogen Spread: Analysis of Residential Wastewater for Infectious Microorganisms including SARS-CoV-2

Kim¹,

Shi

Oh³

et al. 2023

Infect Chemother

Self Cite

View full text Add to dashboard Cite

Background We aimed to identify the genes of 35 pathogens, including viruses, bacteria, and protozoa that cause waterborne infectious diseases, and to assess the feasibility of a wastewater-based surveillance system. Materials and Methods Wastewater was aseptically sampled once a month from 2 sites. A total of 1 L of wastewater from each site underwent 0.2 µm filtration to generate the sample A. Subsequently, 200 ul of the filtered water was ultra-filtered and concentrated to generate the sample B, which was mixed with sample A in a 1:1 ratio. We performed a Filmarray ® Gastrointestinal (GI) panel (BioFire Diagnnostics’, Salt Lake City, UT, USA) test to simultaneously detect 13 enterobacteria, 5 enterovirus, and 4 protozoa. RNA was extracted to assess 18 types of viruses. Results Severe acute respiratory syndrome coronavirus 2 adenovirus, bocavirus, and rhinovirus was detected at both site. Norovirus GI/GII was continuously detected at both sites. Moreover, adenovirus, group A rotaviruses, and hepatitis A virus were frequently detected; however, hepatitis E virus was absent at either site. Campylobacter, enteroaggregative Escherichia coli , enterotoxigenic E. coli , Shiga toxin-producing E. coli , and Clostridioides difficile toxin A/B were detected at both sites. Giardia lamblia was also detected in both sites. Conclusion We analyze multiple infectious disease pathogens under sample surveillance with incidence. Further in-depth studies on wastewater-based surveillance will be feasible and important.

show abstract

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Wastewater Knows Pathogen Spread: Analysis of Residential Wastewater for Infectious Microorganisms including SARS-CoV-2

Kim¹,

Shi

Oh³

et al. 2023

Infect Chemother

Self Cite

View full text Add to dashboard Cite

show abstract

“…In a study in Bangladesh, E. coli levels in food stored at home increased with each 1C increase in the storage area temperature 11 . One study in Korea found increased prevalence of pathogenic E. coli on raw vegetables during months with higher temperatures while a laboratory study did not find temperature effects on bacterial load on vegetables 12,13 . Data on weather effects on other fecal-oral transmission pathways are scarce.…”

Section: Introductionmentioning

confidence: 97%

Effects of weather extremes on fecal contamination along pathogen transmission pathways in rural Bangladeshi households

Niven,

Islam,

Nguyen

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Weather extremes are predicted to influence pathogen exposure but their effects on specific fecal-oral transmission pathways are not well investigated. We evaluated effects of extreme rain and temperature during different antecedent periods (0-14 days) on E. coli along eight fecal-oral transmission pathways in rural Bangladeshi households. Methods: E. coli was enumerated in mother and child hand rinses, food, stored drinking water, tubewells, flies, ponds, and courtyard soil using IDEXX Quanti-Tray/2000 in nine rounds over 3.5 years (n=26,659 samples) and spatiotemporally matched to daily weather data. We used generalized linear models with robust standard errors to estimate E. coli count ratios (ECRs) associated with extreme rain and temperature, defined as >90th percentile of daily values during the study period. Findings: Controlling for temperature, extreme rain on the sampling day was associated with increased E. coli in food (ECR=3.13 (1.63, 5.99), p=0.001), stored drinking water (ECR=1.98 (1.36, 2.88), p<0.0005) and ponds (ECR=3.46 (2.34, 5.11), p<0.0005), and reduced E. coli in soil (ECR=0.36 (0.24, 0.53), p<0.0005). Extreme rain the day before sampling was associated with reduced E. coli in tubewells (ECR=0.10 (0.02, 0.62), p=0.014). Effects were similar for rainfall 1-7 days before sampling and slightly attenuated for rainfall 14 days before sampling. Controlling for rainfall, extreme temperature on the sampling day was associated increased E. coli in stored drinking water (ECR=1.49 (1.05, 2.12), p=0.025) and food (ECR=3.01 (1.51, 6.01), p=0.002). Rainfall/temperature was not consistently associated with E. coli on hands and flies. Interpretation: In rural Bangladesh, measures to control enteric infections following weather extremes should focus on reducing contamination of drinking water and food stored at home and reducing exposure to surface waters. Funding: Bill & Melinda Gates Foundation, National Institutes of Health, World Bank.

show abstract

“…are found in the gastrointestinal tracts of both domestic [6] and wild animals [7], as well as in humans [8]. They are transmitted through feces into agricultural produce [9], particularly leafy vegetables, during irrigation, cultivation, and processing [10], and they can spread from one animal to another (domestic to wild and vice versa) and from one person to another. Moreover, they are a common food-borne pathogen, accounting for 6.3% of E. coli and 3.5% of Salmonella infections in Korea [11].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Salmonella spp. and Escherichia coli in the feces of free-roaming wildlife throughout South Korea

Mafizur,

Sangjin,

Chul

2024

PLoS ONE

View full text Add to dashboard Cite

Wildlife can carry pathogenic organisms, including viruses, bacteria, parasites, and fungi, which can spread to humans and cause mild to serious illnesses and even death. Spreading through animal feces, these pathogens significantly contributes to the global burden of human diseases. Therefore, the present study investigated the prevalence of zoonotic bacterial pathogens, such as Salmonella spp., Escherichia coli, and Shiga toxin-producing E. coli (STEC), in animal feces. Between September 2015 and August 2017, 699 wildlife fecal samples were collected from various agricultural production regions and mountainous areas in South Korea. Fecal samples were collected from wild mammals (85.26%, 596/699) and birds (14.73%, 103/699). Salmonella spp. and E. coli were present in 3% (21/699) and 45.63% (319/699) of the samples, respectively. Moreover, virulence genes stx1 and both stx1 and stx2 were detected in 13.30% (93/699) and 0.72% (5/699) of the samples, respectively. The 21 Salmonella spp. were detected in badgers (n = 5), leopard cats (n = 7), wild boars (n = 2), and magpies (n = 7); STEC was detected in roe deer, water deer, mice, and wild boars. Through phylogenetic and gene-network analyses, the Salmonella spp. isolates (n = 21 laboratory isolates, at least one isolate from each Salmonella-positive animal fecal sample, and n = 6 widely prevalent reference Salmonella serovars) were grouped into two major lineages: S. enterica subsp. enterica and S. enterica subsp. diarizonae. Similarly, 93 E. coli isolates belonged to stx1, including three major lineages (groups 1–3), and stx1 and stx2 detected groups. To the best of our knowledge, this is the first report of a wild leopard cat serving as a reservoir for Salmonella spp. in South Korea. The research findings can help manage the potential risk of wildlife contamination and improve precautionary measures to protect public health.

show abstract

Investigation of Pathogenic Escherichia coli Contamination of Vegetables Distributed in a Korean Agricultural Wholesale Market

Cited by 3 publications

References 21 publications

Wastewater Knows Pathogen Spread: Analysis of Residential Wastewater for Infectious Microorganisms including SARS-CoV-2

Wastewater Knows Pathogen Spread: Analysis of Residential Wastewater for Infectious Microorganisms including SARS-CoV-2

Effects of weather extremes on fecal contamination along pathogen transmission pathways in rural Bangladeshi households

Prevalence of Salmonella spp. and Escherichia coli in the feces of free-roaming wildlife throughout South Korea

Contact Info

Product

Resources

About