Investigation of Panton-Valentine Leukocidin Genes and SCC<i>mec</i>Types in Clinical<i>Staphylococcus aureus</i>Isolates from Turkey

Karahan, Zeynep Ceren; Tekelı, Alper; Adaleti, Rıza; Koyuncu, Esra; Dolapçı, İştar; Akan, Özay Arıkan

doi:10.1089/mdr.2008.0811

Cited by 20 publications

(13 citation statements)

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“…In combination with genotyping of the S. aureus chromosome, the SCC mec typing system has become an important technique for distinguishing between HA-MRSA and CA-MRSA. Most HA-MRSA infections are SCC mec types I, II, and III, while CA-MRSA infections are SCC mec types IV and V. 1 , 2 In this study, two multiplex PCR systems were used: the first was developed by Oliveira and Lencastre , which identified four SCC mec types using characteristic genes in junkyard regions; the second was developed by Zhang et al . 3 , 4 Both systems identified the mec and ccr region, although they differ slightly with respect to primer sequences and target genes.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from Makkah hospitals

Asghar¹

2014

Pak J Med Sci

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Background and Objectives: Methicillin-resistant S. aureus (MRSA) tends to be resistant to multiple antibiotics. Methicillin resistance is conferred by the acquisition of the mecA gene, which is carried by a mobile genetic element called the staphylococcal cassette chromosome mec (SCCmec). There are five major types of SCCmec elements (I–V). The majority of hospital-acquired MRSA (HA-MRSA) strains carry SCCmec types I, II, or III, whereas community-acquired MRSA (CA-MRSA) strains carry SCCmec types IV or V. In addition, Panton-Valentine Leucocidin (PVL) is a gene encoding a powerful cytotoxin that is strongly associated with CA-MRSA strains. The present study was aimed to identify the types of SCCmec and PVL genes among clinical MRSA isolates. Methods: This study was conducted in 5 tertiary care hospitals in Makkah city from March to September of 2012. A total of 206 S. aureus clinical isolates were analysed using standard microbiological methods. Multiplex PCR was performed on genomic DNA from MRSA isolates in order to identify the types of SCCmec. In addition, PCR was performed to detect the PVL gene among the isolates. Results: Of the 206 S. aureus isolates, 114 (55.3%) were MRSA, and 100 of the MRSA isolates carried the mecA gene. Results from SCCmec typing revealed that 3% were type I; 9% were type II; 47% were type III, and 29% were type IV. Nineteen per cent of the isolates harboured the PVL gene. Furthermore, there was a statistically significant correlation between the presence of the PVL gene and SCCmec type IV. Conclusion: The virulence of MRSA strains is increasing in both hospital and community settings in Makkah, highlighting the importance of their rapid identification in order to appropriately control infection.

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Section: Discussionmentioning

confidence: 99%

“…CA-MRSA strains carry SCCmec type IV or V, whereas the majority of HA-MRSA strains carry SCCmec type I, II or III. 1 , 2 …”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from Makkah hospitals

Asghar¹

2014

Pak J Med Sci

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“…The presence of PVL genes in Turkey ranges from 0 to 12.7% 7-10, 27, 28. In this study, PVL was detected in 6.9% of the isolates, and these isolates belonged to three CA-MRSA clones.…”

Section: Discussionmentioning

confidence: 99%

“…The prevalence of community-acquired MRSA appears to be low (1-3%) in Turkey 5,6. Both CA- and HA-MRSA strains in Turkey may contain PVL genes 7-9, and more than 80% of the strains have SCC mec type III (mainly in HA-MRSA) or, less frequently, SCC mec type IIIb, IV and V (the latter two mainly in CA-MRSA) 4, 5, 7, 9, 10. Rare isolates with SCC mec type I and II have also been reported 7.…”

Section: Introductionmentioning

confidence: 99%

The High Diversity of MRSA Clones Detected in a University Hospital in Istanbul

Öksüz¹,

Dupieux²,

Tristan³

et al. 2013

Int. J. Med. Sci.

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Background: To characterize the methicillin-resistant Staphylococcus aureus (MRSA) clones present in Istanbul, 102 MRSA isolates collected during a 5-year period at the Istanbul Medical Faculty Hospital were characterized using microarray analysis and phenotypic resistance profiles.Methods: Resistance to methicillin was detected with a cefoxitin disk diffusion assay and confirmed with a MRSA-agar and MRSA detection kit. Antimicrobial susceptibility testing was performed by a disk diffusion assay and interpreted according to the 2012 guidelines of the Antibiogram Committee of the French Society for Microbiology. Decreased susceptibility to glycopeptides was confirmed using the population analysis profile-area under the curve (PAP-AUC) method. The presence of the mecA gene was detected by polymerase chain reaction. Bacterial DNA was extracted according to the manufacturer's recommended protocol using commercial extraction kits. Strains were extensively characterized using the DNA microarray.Results: Isolates were grouped into six clonal complexes. The most frequently detected clone was the Vienna/Hungarian/Brazilian clone (ST239-MRSA-III), which accounted for 53.9% of the isolates. These isolates were resistant to multiple antibiotics, particularly penicillin, tetracycline, rifampicin, kanamycin, tobramycin, gentamicin, levofloxacin, erythromycin, lincomycin and fosfomycin. Furthermore, three isolates were detected by population analysis profile as heterogeneous vancomycin-intermediate S. aureus (hVISA). The UK-EMRSA-15 clone (ST22-MRSA-IV PVL negative) was detected in 9.8% of the isolates and was mainly susceptible to all anti-staphylococcal antibiotics. Seven isolates (6.9%) were positive for PVL genes and were assigned to the CC80-MRSA-IV clone (European CA-MRSA clone, three isolates), ST8-MRSA-IV clone (USA300 clone, two isolates, one ACME-positive) or ST22-MRSA-IV clone (“Regensburg EMRSA” clone, two isolates). All other clones were detected in one to six isolates and corresponded to well-known clones (e.g., Pediatric clone, Dublin EMRSA clone, WA MRSA-54/63, WA MRSA-1/57).Conclusions: This work highlighted both the high prevalence of ST239-MRSA-III clone and the large diversity of the other MRSA clones detected in a university hospital in Istanbul.

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“…Comparatively, countries in western and central Africa boast a higher prevalence rate at 57%, raising valid concerns for the spread of virulent PVL-positive MRSA clones [11]. Along similar lines, data from across the globe paints a varied picture for the prevalence of PVL-positive S. aureus : 12.8% in China [12], 30% in Germany [13], 1.8% in Ireland [14], 0.9% in Korea [15], 11.6% in Singapore [16], 4% in Turkey [17], and a striking 97% in the United States [18, 19]. …”

Section: Introductionmentioning

confidence: 99%

High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-AcquiredStaphylococcus aureusIsolated from Tertiary Care Hospitals in Nepal

Shrestha

Singh²,

Raj³

et al. 2014

BioMed Research International

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Methicillin-resistant Staphylococcus aureus (MRSA) carrying the important virulence determinant, Panton-Valentine leukocidin (PVL), is an emerging infectious pathogen associated with skin and soft tissue infections as well as life-threatening invasive diseases. In carrying out the first PVL prevalence study in Nepal, we screened 73 nosocomial isolates of S. aureus from 2 tertiary care Nepali hospitals and obtained an overall PVL-positivity rate of 35.6% among the hospital isolates: 26.1% of MRSA and 51.9% of methicillin sensitive S. aureus (MSSA) isolates were found to be positive for the PVL genes. PVL prevalence was not associated with a specific (i) infection site, (ii) age group, or (iii) hospital of origin. It was found to be positively associated with heterogeneous MRSA (73.3%) compared to homogeneous MRSA (3.2%) and MSSA (51.9%); negatively associated with multiresistant MRSA (22%) compared to nonmultiresistant MRSA (60%) and MSSA (51.9%); and positively associated with macrolide-streptogramin B resistance (93.8%) compared to macrolide-lincosamide-streptogramin B resistance (0%) and no-resistance (45.8%) types. Macrolide-streptogramin B resistance was confirmed by the presence of msr(A) gene. Restriction pattern analyses provided evidence to support the circulation of a limited number of clones of PVL-positive MRSA, arguing for the adaptability of these isolates to a hospital setting.

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Investigation of Panton-Valentine Leukocidin Genes and SCCmecTypes in ClinicalStaphylococcus aureusIsolates from Turkey

Cited by 20 publications

References 52 publications

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from Makkah hospitals

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from Makkah hospitals

The High Diversity of MRSA Clones Detected in a University Hospital in Istanbul

High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-AcquiredStaphylococcus aureusIsolated from Tertiary Care Hospitals in Nepal

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