Investigation of Oxygen Consumption by E. coli Immobilized in a Synthetic Biofilm Using a Thin Film Plug Reactor (TFPR)

Thiagarajan, V.; Swope, Kristi L.; Flickinger, Michael C.

doi:10.1016/s0921-0423(96)80041-4

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…Numerous in vitro studies can be found in the literature based on the Bioelectrochemistry 105 (2015) [56][57][58][59][60][61][62][63][64] relative impedance changes induced after cell adhesion onto the electrodes [14,15], some of them using commercial interdigitated microelectrodes (IDuE) [16,17]. Despite offering a large sensitive area in a limited space, most of commercial IDuE do not offer the possibility of studying cell growth under flow conditions, a variable that is known to affect biofilm structure and behavior [18], or to measure additional cell culture parameters to control other cell cultures analytes of interest [19]. Furthermore, not many studies have been published on biofilm growth monitoring in a microfluidic channel using IDuEs [20][21][22][23], a technology that deserves further study and refinement.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous monitoring of Staphylococcus aureus growth in a multi-parametric microfluidic platform using microscopy and impedance spectroscopy

Estrada-Leypon

Moya

Guimerà‐Brunet

et al. 2015

Bioelectrochemistry

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Section: Introductionmentioning

confidence: 99%

Simultaneous monitoring of Staphylococcus aureus growth in a multi-parametric microfluidic platform using microscopy and impedance spectroscopy

Estrada-Leypon

Moya

Guimerà‐Brunet

et al. 2015

Bioelectrochemistry

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“…Because latex biocatalytic coatings are adhesive and contain a very high number of bacteria, many types of small volume bioreactors containing single patches or strip coatings have been devised for reactivity and kinetic studies. Initial E. coli studies of bilayer coatings used 1.3 cm squares of coated polyester with sealed edges (40 − 43) or 45 cm 2 circular coatings divided into sectors on the surface of 72 mm diameter aluminum plugs in a stirred, jacketed cell−thin film plug reactor (TFPR) (62). Oxygen uptake was used as a measure of E. coli viability following coat drying under different conditions and rehydration (62, 63).…”

Section: 0 Methods For Investigating Microbial Biocatalytic Coatingsmentioning

confidence: 99%

“…Initial E. coli studies of bilayer coatings used 1.3 cm squares of coated polyester with sealed edges (40 − 43) or 45 cm 2 circular coatings divided into sectors on the surface of 72 mm diameter aluminum plugs in a stirred, jacketed cell−thin film plug reactor (TFPR) (62). Oxygen uptake was used as a measure of E. coli viability following coat drying under different conditions and rehydration (62, 63). Subsequently, 12.7 and 47 mm diameter E. coli patches were made using pressure sensitive vinyl holey masks formed by circular punches (46, 54), which simplified sealing of the edges (Figure 4).…”

Section: 0 Methods For Investigating Microbial Biocatalytic Coatingsmentioning

confidence: 99%

Painting and Printing Living Bacteria: Engineering Nanoporous Biocatalytic Coatings to Preserve Microbial Viability and Intensify Reactivity

Flickinger

Schottel

Bond

et al. 2007

Biotechnology Progress

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100

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Latex biocatalytic coatings containing ∼50% by volume of microorganisms stabilize, concentrate and preserve cell viability on surfaces at ambient temperature. Coatings can be formed on a variety of surfaces, delaminated to generate stand-alone membranes or formulated as reactive inks for piezoelectric deposition of viable microbes. As the latex emulsion dries, cell preservation by partial desiccation occurs simultaneously with the formation of pores and adhesion to the substrate. The result is living cells permanently entrapped, surrounded by nanopores generated by partially coalesced polymer particles. Nanoporosity is essential for preserving microbial viability and coating reactivity. Cryo-SEM methods have been developed to visualize hydrated coating microstructure, confocal microscopy and dispersible coating methods have been developed to quantify the activity of the entrapped cells, and FTIR methods are being developed to determine the structure of vitrified biomolecules within and surrounding the cells in dry coatings. Coating microstructure, stability and reactivity are investigated using small patch or strip coatings where bacteria are concentrated 10 2 -to 10 3 -fold in 5-75 µm thick layers with pores formed by carbohydrate porogens. The carbohydrate porogens also function as osmoprotectants and are postulated to preserve microbial viability by formation of glasses inside the microbes during coat drying; however, the molecular mechanism of cell preservation by latex coatings is not known. Emerging applications include coatings for multistep oxidations, photoreactive coatings, stabilization of hyperthermophiles, environmental biosensors, microbial fuel cells, as reaction zones in microfluidic devices, or as very high intensity (>100 g‚L -1 coating volume‚h -1 ) industrial or environmental biocatalysts. We anticipate expanded use of nanoporous adhesive coatings for prokaryotic and eukaryotic cell preservation at ambient temperature and the design of highly reactive "living" paints and inks.

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“…[10,11] The idea of encapsulating viable bacteria in a latex coating first appeared in the 1980s. [12] In 1996, landmark publications that systematically studied and characterized biocoatings were published by Flickinger and colleagues, [13][14][15] who later coined the term "biocoating" [10] to refer to an adhesive biocatalytic coating. Biocoatings or "living paints" are attracting growing interest, but there are to-date only about 50 publications on the topic.…”

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confidence: 99%

Waterborne Coatings Encapsulating Living Nitrifying Bacteria for Wastewater Treatment

Chen

Krings

Beale

et al. 2022

Advanced Sustainable Systems

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Biofilm bioreactors are attracting growing interest in the wastewater industry, as they allow higher cell densities and thus higher reaction rates compared to conventional bioreactors. However, some commonly used nitrifying bacteria, such as Nitrosomonas europaea, are slow‐growing and need a prolonged period of time to develop a mature biofilm. Here, a biocoating or “living paint” is introduced, which is a synthetic biofilm made from a colloidal polymer (synthetic latex) binder encapsulating viable nitrifying bacteria at high density. Conventionally, the film formation of biocoatings is achieved by drying a bacteria/latex mixture. However, this fabrication is detrimental to the viability of the encapsulated bacteria because of the osmotic stress induced by desiccation. A nondesiccating film formation process is presented for biocoatings, which exploits two colloid science phenomena: coagulation and wet sintering. Desiccation‐sensitive, nitrifying bacteria are employed in the biocoatings to convert NH4+ to NO2− and then NO3−. These biocoatings have a conversion rate (NO2− and NO3− production) of 3 mg N g−1 d−1 that is five times higher than in conventionally desiccated biocoatings. The reactivity continues over a period of 1 month. The processing method for these living paints is transformative for wastewater treatment and other applications using delicate, desiccation‐sensitive microorganisms.

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Investigation of Oxygen Consumption by E. coli Immobilized in a Synthetic Biofilm Using a Thin Film Plug Reactor (TFPR)

Cited by 4 publications

References 6 publications

Simultaneous monitoring of Staphylococcus aureus growth in a multi-parametric microfluidic platform using microscopy and impedance spectroscopy

Simultaneous monitoring of Staphylococcus aureus growth in a multi-parametric microfluidic platform using microscopy and impedance spectroscopy

Painting and Printing Living Bacteria: Engineering Nanoporous Biocatalytic Coatings to Preserve Microbial Viability and Intensify Reactivity

Waterborne Coatings Encapsulating Living Nitrifying Bacteria for Wastewater Treatment

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