Abstract:nanoparticle-protein conjugates are not easily crystallized. Previous work from our group suggests that the GB3 protein remains globular when adsorbed to gold nanoparticles (AuNPs), but it is unclear whether the tertiary structure is retained. Here, we apply several novel NMR-based approaches to probe the structure and orientation of GB3 bound to AuNPs. We have developed a method for monitoring hydrogen-deuterium exchange (HDX) on the AuNP surface, and we find that HDX rates of surface-bound GB3 are highly cor… Show more
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