Investigation of Oligosaccharides for Prebitoic Action on Vaginal Lactobacilli

Abstract

Objective: Vaginitis is a very common gynaecological problem in women of all age groups resulted in millions of visit to physician. Decrease in vaginal Lactobacilli is one of the reasons of Bacterial vaginitis. Approach that could improve decreased level of Lactobacilli and inhibit growth of pathogenic bacteria can be alternative to antibiotic therapy. Oligosaccharides influence the growth of Lactobacilli species and benefits vaginal ecosystem. The present study was designed to evaluate prebitoic potential of oligosaccharides in vaginitis. Methodology: The potential of oligosaccharides to stimulate the growth of three selected vaginal Lactobacilli strains was studied by optical density, pH, titrable acidity and dry mass after 48 h of incubation. The antimicrobial effect of vaginal Lactobacilli in presence of oligosaccharide against pathogenic bacteria E. coli and C. albicans was studied by agar diffusion method. Vaginal irritation test was studied on female Swiss albino mice. Results and Discussion: Increased lactic acid production and lowering of pH by Lactobacilli strains in presence of oligosaccharides confirmed that oligosaccharide can stimulate the growth of selected Lactobacilli species. Optical density and dry biomass was also increased during 48 h of incubation. Higher zone of inhibition was observed in presence of prebiotic compared to control (without prebiotic). Vaginal irritation study showed no significant changes. Conclusively, oligosaccharides supported the growth of selected strains and could restore bacterial environment.

The Role of Fatty Acid Metabolites in Vaginal Health and Disease: Application to Candidiasis

“…A vaginal bio-adhesive delivery system based on pectinate-hyaluronic acid microparticles for prebiotics and probiotics encapsulation was designed for better controlled drug release in the vaginal tract (Pliszczak et al, 2011). An in vitro study showed that FOS and GOS stimulate the growth of lactobacilli, generating lactate and resulting in lower pH, causing suppressing of growth of harmful species like E. coli and potentially C. albicans in the vaginal ecosystem (Mahore et al, 2017). Other studies show that the prebiotics glucomannan and glucomannan hydrolysates (GMH) promote the growth, metabolism and antimicrobial properties Lactobacillus strains, with an increased inhibition of vaginal Candida species and link this to the produced lactic acid (Huang et al, 2007;Sutherland et al, 2008;Tester et al, 2012).…”

Effect of Roasting on Prebiotic Potential of Soyabean (Glycine Max)

“…Significant increases in the inhibition of E. coli was observed in presence of raw and roasted Soyabean compared to control (without prebiotic). Higher zone of inhibition was observed in presence of prebiotic compared to control (without prebiotic) 15 . Production of metabolic by-products such as fatty acids, hydrogen peroxide, hydrogen and hydroxyl ions, and ammonia, as well as bacteriocins and bacteriocin-like substances are responsible to antimicrobial activity of Lactobacilli.…”

In vitro and in vivo evaluation of prebiotic potential of pectin on vaginal lactobacilli

“…The flasks were then incubated at 37°C for 48 h in anaerobic condition. Samples were collected after every 2 h to determine optical density at 600 nm [20]. Each experiment was conducted in triplicate.…”

“…A clinical study by Coste et al (2012) [12] indicated recovery of normal vaginal flora in patients treated with gels containing glucooligosaccharides. In one of our previous studies, the in vitro effect of fructo-and galacto-oligosaccharides on vaginal lactobacilli such as Lactobacilli acidophilus, Lactobacilli casei (LC), and Lactobacilli fermentum (LF) was investigated [20]. In the present study, an attempt has been made to investigate the in vitro and in vivo effect of pectin on vaginal lactobacilli.…”