Investigation of Obesity Candidate Genes On Porcine Fat Deposition Quantitative Trait Loci Regions

Kim, Kwan‐Suk; Thomsen, Hauke; Bastiaansen, J.W.M.; Nguyen, Nguyet Thu; Dekkers, Jack C. M.; Plastow, Graham; Rothschild, Max F.

doi:10.1038/oby.2004.249

Cited by 71 publications

(49 citation statements)

References 47 publications

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“…Kim et al (2004) found single polymorphism in the third exon of porcine GHRL gene, which was correlated with a several porcine traits, such as marbling, backfat thickness and weights of loin and ham. Jin et al (2010) investigated several polymorphisms in caprine ghrelin gene and they confirmed association between mutation in second intron (G147A) and growth traits such as: circumference of chest and cannon, and also trunk index (P<0.05 and P<0.01).…”

Section: Discussionmentioning

confidence: 99%

“…In the pig, single polymorphism in third exon has been found so far and according to the authors this mutation is associated with several economically important traits (Kim et al, 2004). The aim of our study was to identify new polymorphisms in the porcine GHRL gene -in exons and in the regulatory regions (promoter region, 5' and 3' untranslated regions).…”

Section: Introductionmentioning

confidence: 99%

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New polymorphisms and expression of the porcine ghrelin (<i>GHRL</i>) gene in different pig breeds

Ropka‐Molik¹,

Oczkowicz²,

Piórkowska³

et al. 2011

J. Anim. Feed Sci.

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Growth and development traits are economically important in animal production, especially in pig breeding. Therefore, the porcine GHRL gene is considered as a candidate gene responsible for growth rate and body weight. The aim of our study was to identify new polymorphisms in the GHRL gene in pig. Ten novel single nucleotide polymorphisms (SNP's) were detected: four substitutions in exons, four in introns and two mutations in promoter region. We evaluated the GHRL mRNA abundance in porcine stomachs (fundus ventriculis) and ghrelin protein concentration in plasma in three breeds: Polish Landrace, Polish Large White and Pietrain. The results showed that transcript abundance of GHRL gene was significantly higher in Polish Landrace than in other two breeds (P<0.05). The mutation c.-93A>G located in the promoter region affected expression of the GHRL gene. The AA genotype animals showed a significantly (P<0.05) higher expression level when compared to AG genotype animals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New polymorphisms and expression of the porcine ghrelin (<i>GHRL</i>) gene in different pig breeds

Ropka‐Molik¹,

Oczkowicz²,

Piórkowska³

et al. 2011

J. Anim. Feed Sci.

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“…Two biological candidate genes (ESR and PRLR ) have shown significant association with litter size (Short et al, 1997;Tomas et al, 2006). An MC4R mutation has shown a significant reduction in feed intake with less fat deposition (Kim et al, 2000a;Kim et al, 2004). Additional important genes, PRKAG3 and CAST, have been shown to be associated with changes in pH and tenderness (Ernst et al, 1998;Ciobanu et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Development of Optimal Breeding Pigs Using DNA Marker Information

Kim¹,

Roh²,

Cho³

et al. 2010

Genomics & Informatics

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The aim of the study was to investigate pig reference families, generated from Korean native pigs (KNP) that were crossed with Yorkshire (YS) breeds, which were used to evaluate genetic markers to select breeding animals with superior pork quality. A set of five candidate genes (PRKAG3, MC4R, CAST, ESR, and PRLR ) was analyzed for association with pork quality traits. PRKAG3 (I199V) SNP genotypes were significantly associated with muscle moisture, protein, and fat contents.The MC4R D298N polymorphism was significantly associated with meat tenderness and color traits. The CAST polymorphism was significantly associated with muscle moisture and crude protein traits. These three genes have been associated with pork quality traits in other pig populations, and some of our results are consistent with earlier studies. In addition, two reproductive candidate genes (ESR and PRLR ) did not have significant associations. These results suggest that further study is warranted to investigate and develop more DNA markers associated with pork quality in our KNP-crossed pig families.

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“…Preliminary studies on the swine genome have revealed that it is more highly similar to humans than rodents (Wernersson et al, 2005;Hart et al, 2007). We have found that genes involved in obesity-related phenotypes in humans, such as the melanocortin-4 receptor (MC4R) and high-mobility group AT-hook 1 (HMGA1) genes, were associated with fatness traits in pigs (Kim et al, 2000;Kim et al, 2004). Most recently, transcription factor-7-like 2 (TCF7L2) and fat mass and obesityassociated (FTO) genes implicated in T2D in different human cohort studies have been found to be associated with fat and growth rate traits in pigs (Fontanesi et al, 2008;Du et al, 2009;Fan et al, 2009).…”

Section: Introductionmentioning

confidence: 91%

The Hepatocyte nuclear factor-1 alpha (HNF1A) gene is associated with fatness and loin muscle area in the pig

Fan

Rothschild

2010

Animal

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The significance of hepatocyte nuclear factors (HNFs) in b-cell development and function has been generally recognized in humans, as evidenced by their associations with cases of maturity onset diabetes of the young (MODY). Common Hepatocyte nuclear factor-1 alpha (HNF1A), Hepatocyte nuclear factor-1 beta ( HNF1B) and Hepatocyte nuclear factor-4 alpha ( HNF4A) mutations could lead to monogenic forms 3, 5 and 1 of diabetes mellitus, respectively, and were characterized by MODY in humans. In this study, multiple variants were discovered in the porcine HNF1A and HNF4A genes, and one single-nucleotide polymorphism (SNP) was detected in the HNF1B gene. Using the Iowa State University Berkshire 3 Yorkshire pig resource population, the HNF1A, HNF1B and HNF4A genes were mapped on chromosomes 14, 12 and 17, respectively. The linkage disequilibrium (LD) analyses indicated that most of the HNF1A variants were not in strong LD with each other; however, nearly all of them were highly significantly ( P , 0.01) associated with loin muscle area (LMA). The SNPs c.327-19G.T and c.1768140_23del were significantly ( P , 0.05) associated with backfat and total lipid percentage, and the latter was also associated with muscle glycogen metabolism measures. Four major haplotypes were observed and the association analyses suggested that haplotype 3 (-CGCGD-, I indicates Insertion and D indicates Deletion) was favorable for reduced backfat, while haplotype 1 (-CACGI-) was unfavorably associated with backfat and LMA. There was no significant interaction effect on backfat among the SNPs c.327-19G.T, c.1768140_23del of the HNF1A gene and c.646C.T of the transcription factor-7-like 2 (TCF7L2) gene. These findings suggest that the HNF1A gene has significant effects on both fatness-and meat production-related traits. No significant associations with production traits with the SNPs from the HNF1B and HNF4A genes were observed in the study.

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Investigation of Obesity Candidate Genes On Porcine Fat Deposition Quantitative Trait Loci Regions

Cited by 71 publications

References 47 publications

New polymorphisms and expression of the porcine ghrelin (<i>GHRL</i>) gene in different pig breeds

New polymorphisms and expression of the porcine ghrelin (<i>GHRL</i>) gene in different pig breeds

Development of Optimal Breeding Pigs Using DNA Marker Information

The Hepatocyte nuclear factor-1 alpha (HNF1A) gene is associated with fatness and loin muscle area in the pig

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