Investigation of Microbubble Cavitation-Induced Calcein Release from Cells In Vitro

Maciulevičius, Martynas; Tamošiūnas, Mindaugas; Jakštys, Baltramiejus; Jurkonis, Rytis; Venslauskas, Mindaugas Saulius; Šatkauskas, Saulius

doi:10.1016/j.ultrasmedbio.2016.08.005

Cited by 9 publications

(7 citation statements)

References 85 publications

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“…Using the proposed setup, high intensity ultrasounds (380 kPa, 40% duty cycle, and 10 kHz pulse repetition frequency) were delivered. Under these sonication conditions, the microbubbles cavitate and explode in several cycles: In less than 2 s, no microbubble was found in the solution anymore [28]. Thus, at this pressure, long sonication times are not necessary to study the US/MB interaction.…”

Section: Experimental Results Using the New Setupmentioning

confidence: 99%

A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization

et al. 2021

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Sonoporation is the process of cell membrane permeabilization, due to exposure to ultrasounds. There is a lack of consensus concerning the mechanisms of sonoporation: Understanding the mechanisms of sonoporation refines the choice of the ultrasonic parameters to be applied on the cells. Cells’ classical exposure systems to ultrasounds have several drawbacks, like the immersion of the cells in large volumes of liquid, the nonhomogeneous acoustic pressure in the large sample, and thus, the necessity for magnetic stirring to somehow homogenize the exposure of the cells. This article reports the development and characterization of a novel system allowing the exposure to ultrasounds of very small volumes and their observation under the microscope. The observation under a microscope imposes the exposure of cells and Giant Unilamellar Vesicles under an oblique incidence, as well as the very unusual presence of rigid walls limiting the sonicated volume. The advantages of this new setup are not only the use of a very small volume of cells culture medium/microbubbles (MB), but the presence of flat walls near the sonicated region that results in a more homogeneous ultrasonic pressure field, and thus, the control of the focal distance and the real exposure time. The setup presented here comprises the ability to survey the geometrical and dynamical aspects of the exposure of cells and MB to ultrasounds, if an ultrafast camera is used. Indeed, the setup thus fulfills all the requirements to apply ultrasounds conveniently, for accurate mechanistic experiments under an inverted fluorescence microscope, and it could have interesting applications in photoacoustic research.

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Section: Experimental Results Using the New Setupmentioning

confidence: 99%

A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization

et al. 2021

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Section: Effects Of Ultrasound On Cell Membrane Permeabilitymentioning

confidence: 99%

“…Sonoporation describes the progressive opening of the cell membrane due to microbubble cavitation upon US exposure of cells (Lentacker, De Cock, Deckers, De Smedt, & Moonen, 2014;Maciulevičius et al, 2016). The micro-bubbles create micro-streaming and/or liquid jets (Maciulevičius et al, 2016), which generate a strong shear force that breaks the chemical bonds in the cell membranes (Tabatabaie & Mortazavi, 2008) (Gao, Hemar, Ashokkumar, Paturel, & Lewis, 2014a;Gao, Lewis, Ashokkumar, & Hemar, 2014b), which play a vital role in lipid bilayer relocation and membrane disruption through lipid peroxidation. Furthermore, it was also revealed that peroxidation of membrane lipids (Ewe et al, 2012;Lentacker et al, 2014) and conformational unfolding of proteins that are located on the surface of the cell membrane increase membrane fluidity and membrane permeabilisation upon US treatment (Ewe et al, 2012).…”

Section: Effects Of Ultrasound On Cell Membrane Permeabilitymentioning

confidence: 99%

Effects of ultrasound on the fermentation profile of fermented milk products incorporated with lactic acid bacteria

Abesinghe

Islam

Vidanarachchi

et al. 2019

International Dairy Journal

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Ultrasonic processing of fermented milk products has created much interest in current research on dairy products. This has been employed in cultured milk products to enhance the emulsification of milk fat and to intensify the fermentation process. Benefits including remarkable product stability, reduced processing time and enhanced quality are being recorded. Ultrasound (US) altered the colour and flavour profile of milk; however, the effect of USinduced fermentation on the synthesis of flavour compounds in milk has not been reported in the literature. This review paper presents a comprehensive scenario on the impact of power US on García, 2011). Depending on the conditions used such as amplitude, temperature, pressure, and the composition of the medium, several mechanisms can be activated including increase of the temperature, surface instability, generation of agitation and friction, increase of mass transfer, generation of free radicals and disruption of cell materials (Ashokkumar, 2011; Martini, 2013b; Salazar, Chávez, Turó, & García-Hernández, 2009). 3. Application of power ultrasound in lactic fermentation of milk Application of both low power ultrasound (LPU) and power US in fermentation has been reported in the literature. LPU has power intensities below 1 Wcm-2 and is commonly used for non-destructive analysis in the food industry to characterise food components, often on quality assurance lines and to monitor fermentation processes (Novoa-Díaz et al., 2014) and is not a focus for this review paper. On the other hand, PU (with power intensities above 10 Wcm-2) alone (sonication) or in combination with external pressure (manosonication), heat

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“…Although the use of ultrasound to amplify biomarker signals in the blood was proposed in 2009 24 , most previous studies used high-intensity focused ultrasound (HIFU) to induce permanent mechanical or thermal disruption of tumors outside the brain to liberate biomarkers from tumor cells [25][26][27] . Following the initial introduction of the ultrasound-mediated biomarker amplification concept by D' Souza et al in 2009, several in vitro studies were reported over the next few years [28][29][30][31] . These studies showed that ultrasound combined with microbubble-induced sonoporation could liberate various cellular contents into the extracellular space, such as enhanced green fluorescence protein 28 , mammaglobin mRNA 28 , micro-RNA 21 29 , cancer antigens 125 and 19-9 30 , and small molecule calcein 31 .…”

mentioning

confidence: 99%

“…Following the initial introduction of the ultrasound-mediated biomarker amplification concept by D' Souza et al in 2009, several in vitro studies were reported over the next few years [28][29][30][31] . These studies showed that ultrasound combined with microbubble-induced sonoporation could liberate various cellular contents into the extracellular space, such as enhanced green fluorescence protein 28 , mammaglobin mRNA 28 , micro-RNA 21 29 , cancer antigens 125 and 19-9 30 , and small molecule calcein 31 .…”

mentioning

confidence: 99%

Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model

Pacia

Zhu

Yang

et al. 2020

Sci Rep

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Although blood-based liquid biopsy is a promising noninvasive technique to acquire a comprehensive molecular tumor profile by detecting cancer-specific biomarkers (e.g. DNA, RNA, and proteins), there has been limited progress for brain tumor application partially because the low permeability of the blood-brain barrier (BBB) hinders the release of tumor biomarkers. We previously demonstrated focused ultrasound-enabled liquid biopsy (FUS-LBx) that uses FUS to increase BBB permeability in murine glioblastoma models and thus enhance the release of tumor-specific biomarkers into the bloodstream. The objective of this study was to evaluate the feasibility and safety of FUS-LBx in the normal brain tissue of a porcine model. Increased BBB permeability was confirmed by the significant increase (p = 0.0053) in K trans (the transfer coefficient from blood to brain extravascular extracellular space) when comparing the FUS-sonicated brain area with the contralateral non-sonicated area. Meanwhile, there was a significant increase in the blood concentrations of glial fibrillary acidic protein (GFAP, p = 0.0074) and myelin basic protein (MBP, p = 0.0039) after FUS sonication as compared with before FUS. There was no detectable tissue damage by t 2 *-weighted MRi and histological analysis. findings from this study suggest that FUS-LBx is a promising technique for noninvasive and localized diagnosis of the molecular profiles of brain diseases with the potential to translate to the clinic. Tissue biopsy has been used to characterize and track the tumor molecular landscape. However, tissue biopsy for brain tumor diagnosis requires invasive surgical procedures, which carry a 5-7% risk of major morbidity 1. It may not be possible at all to perform this procedure on medically inoperable patients or patients with tumors in surgically inaccessible locations. Repeated tissue biopsies to assess treatment response and cancer recurrence are often not feasible given the increased risk for complications and morbidity. These challenges limit the timely diagnosis and selection of treatment options, hinder a better understanding of the disease, and impair the development of effective treatment approaches. Liquid biopsy (LBx), which refers to the detection of tumor-derived components in body fluids (e.g., blood, urine, saliva, ascitic fluid, cerebrospinal fluid, etc.), has been gaining enormous attention in both medical research and clinical applications 2,3. Various substances from liquid biopsies have been found to be closely related to the stage of a tumor and might serve as biomarkers for cancer diagnosis and prognosis, such as circulating tumor cells, circulating tumor DNAs, RNAs, extracellular vesicles, and a series of cancer-related proteins 4. Blood-based LBx enables physicians to noninvasively interrogate the dynamic evolution of a tumor and monitor a patient's response to therapies through a simple blood test. Blood-based LBx-guided personalized therapy has already entered clinical practice in the management of several cancers. An import...

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Investigation of Microbubble Cavitation-Induced Calcein Release from Cells In Vitro

Cited by 9 publications

References 85 publications

A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization

A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization

Effects of ultrasound on the fermentation profile of fermented milk products incorporated with lactic acid bacteria

Feasibility and safety of focused ultrasound-enabled liquid biopsy in the brain of a porcine model

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