Investigation of Measurement Methods of Antioxidant Activity and Involved Mechanisms

Abstract

Bioactive food components are active ingredients in food or dietary supplements proven to have a role in health and they are safe for human consumption. These compounds exert their antioxidant effects by different mechanisms such as hydrogen atom transfer (HAT) or single electron transfer (SET) and their efficiencies can be evaluated by several methods such as ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC), dipheny-picrylhydrazil (DPPH), Folin-Ciocaltue method (FCM), etc. In this review, these mechanisms and methods will be discussed in details.

Evaluation of the wound healing activity of twelve herbal and non-herbal remedies used in Sana'a-Yemen for the treatment of wounds and burns

“…HATbased methods measure the ability of antioxidants to disable free radicals by hydrogen donating. SET-based methods detect the ability of antioxidants to transfer single electron to reduce any compound, including metals, carbonyls, and radicals [264,265]. Ten of the herbal and non-herbal remedies extracts and/or their chemical constituents (Table 2) were found to possess antioxidant activity and their ability to scavenge free radicals, reduce metals, chelate metal catalysts, and activate antioxidant enzymes and prevent lipid peroxidation has been proved by several in vivo and in vitro assays.…”