Investigation of mammalian cells expressing SARS-CoV-2 proteins by surface-enhanced Raman scattering and multivariate analysis

Akdeniz, Munevver; Ciloglu, Fatma Uysal; Tunc, Cansu Umran; Yilmaz, Ummugulsum; Kanarya, Dilek; Atalay, Pinar Buket; Aydın, Ömer

doi:10.1039/d1an01989a

Cited by 9 publications

(8 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Understanding the process of viral attachment to, entry into, replication within, and release from cells is a critical aspect of infection control, understanding the mechanism of action of emerging viruses, and monitoring their mutation to new strains. The requirements for Biological Safety Level 3 (BCL-3) laboratory conditions has largely precluded the in situ study of SARS-CoV-2 attachment to, invasion of and replication in cells, although Akdeniz et al have recently developed a virus-infected HEK293 cell model, transfected with plasmids encoding the nucleocapsid, membrane and envelope proteins of SARS-CoV-2 [186] . Gold nanoparticle SERS combined with PCA identified largely protein markers which differentiated transfected and nontransfected cells although the process of transfection was not explicitely studied.…”

Section: Vibrational Spectroscopic Analysis Of Viruses and Viral Infe...mentioning

confidence: 99%

Contributions of vibrational spectroscopy to virology: A review

Chaudhary¹,

Jackson²,

Denning³

et al. 2022

Clinical Spectroscopy

View full text Add to dashboard Cite

Section: Vibrational Spectroscopic Analysis Of Viruses and Viral Infe...mentioning

confidence: 99%

Contributions of vibrational spectroscopy to virology: A review

Chaudhary¹,

Jackson²,

Denning³

et al. 2022

Clinical Spectroscopy

View full text Add to dashboard Cite

“…The following supporting information [27,[37][38][39][40][41] can be downloaded at https://www.mdpi.com/article/10.3390/bios12050300/s1, Supplementary Tables: Supplementary Table S1: spike glycoprotein RBD amino acid sequence, Supplementary Table S2: physico-chemical properties of RBD protein, Supplementary Figure S1: scanning electron microscopy photograph.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Label-Free Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy

et al. 2022

View full text Add to dashboard Cite

Surface-enhanced Raman scattering (SERS) spectroscopy is a surface- or cavity-enhanced variant of Raman scattering spectroscopy that allows the detection of analytes with a sensitivity down to single molecules. This method involves the use of SERS-active surfaces or cavities capable of concentrating incident radiation into small mode volumes containing the analyte. Here, we have engineered an ultranarrow metal–dielectric nano-cavity out of a film of the receptor-binding domain (RBD) of SARS-CoV-2 spike (S) glycoprotein and a silver surface, held together by interaction between reduced protein sulfhydryl groups and silver. The concentration of light in this nano-cavity allows the label-free recording of the characteristic Raman spectra of protein samples smaller than 1 pg. This is sufficient for the ultrasensitive detection of viral protein antigens at physiologically relevant levels. Moreover, the protein SERS signal can be increased by several orders of magnitude by coating the RBD film with a nanometer-thick silver shell, thereby raising the cavity Q-factor. This ensures a sub-femtogram sensitivity of the viral antigen detection. A simple theoretical model explaining the observed additional enhancement of the SERS signal from the silver-coated protein is proposed. Our study is the first to obtain the characteristic Raman and SERS spectra of the RBD of S glycoprotein, the key SARS-CoV-2 viral antigen, directly, without the use of Raman-reporter molecules. Thus, our approach allows label-free recording of the characteristic spectra of viral antigens at concentrations orders of magnitude lower than those required for detecting the whole virus in biological media. This makes it possible to develop a high-performance optical detection method and conformational analysis of the pathogen and its variants.

show abstract

“…Nanoparticle-based drug delivery systems can be the answer to many of the limitations of chemotherapy with the ability to disperse hydrophobic drugs in water and selective distribution through the tumor tissue due to enhanced permeability and retention (EPR) effects. Gold nanoparticles (AuNPs), one important class of nanosized materials, have been utilized for many biomedical applications, including but not limited to sensing, bioimaging, gene delivery, and drug delivery. , Fundamental properties of AuNPs such as low cytotoxicity, minimal immune response, high surface area, tunable size, easy modification, and high cellular internalization make them excellent vehicles for nanomedicine applications, especially for drug delivery …”

Section: Introductionmentioning

confidence: 99%

“…Gold nanoparticles (AuNPs), one important class of nanosized materials, have been utilized for many biomedical applications, including but not limited to sensing, bioimaging, gene delivery, and drug delivery. 22,23 Fundamental properties of AuNPs such as low cytotoxicity, minimal immune response, high surface area, tunable size, easy modification, and high cellular internalization make them excellent vehicles for nanomedicine applications, especially for drug delivery. 27 In this study, two Naproxen 1,2,4-triazol-derived compounds (NDC-1 and NDC-2), from our previously published research 15 were investigated as drugs for treatment of ovarian cancer by attaching these hydrophobic compounds to AuNPs for high aqueous dispersion.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Gold Nanoparticle Naproxen-Derived Conjugations in Ovarian Cancer

et al. 2023

Self Cite

View full text Add to dashboard Cite

Ovarian cancer, which is one of the most diagnosed cancer types among women, maintains its significance as a global health problem. Several drug candidates have been investigated for the potential treatment of ovarian cancer. Nonsteroidal anti-inflammatory drugs (NSAIDs) demonstrated anti-cancer activity through the inhibition of cyclooxygenase 2 (COX-2) and by inhibiting COX-2-dependent prostaglandin (PG) production. Naproxen is one of the most used NSAIDs and Naproxen-derived compounds (NDCs) may show potential treatment effects on cancer as chemotherapeutic drugs. Although there are successful drug development studies, the lack of solubility of these drug candidates in aqueous media results in limited bioavailability and high variability of patient responses during treatment. Low aqueous solubility is one of the main problems in the pharmaceutical industry in terms of drug development. Nanotechnology-based strategies provide solutions to hydrophobic drug limitations by increasing dispersion and improving internalization. In this study, two different NDCs (NDC-1 and NDC-2) bearing a thiosemicarbazide/1,2,4-triazole moiety were synthesized and tested for chemotherapeutic effects on ovarian cancer cells, which have a high COX-2 expression. To overcome the limited dispersion of these hydrophobic drugs, the drug molecules were conjugated to the surface of 13 nm AuNPs. Conjugation of drugs to AuNPs increased the distribution of drugs in aqueous media, and NDC@AuNP conjugates exhibited excellent colloidal stability for up to 8 weeks. The proposed system demonstrated an increased chemotherapeutic effect than the free drug counterparts with at least 5 times lower IC50 values. NDC@AuNP nanosystems induced higher apoptosis rates, which established a simple and novel way to investigate activity of prospective drugs in drug discovery research.

show abstract

Investigation of mammalian cells expressing SARS-CoV-2 proteins by surface-enhanced Raman scattering and multivariate analysis

Abstract: COVID-19 has caused millions of cases and deaths all over the world since late 2019. Rapid detection of the virus is crucial to control its spread through a population. COVID-19...

Cited by 9 publications

References 56 publications

Contributions of vibrational spectroscopy to virology: A review

Contributions of vibrational spectroscopy to virology: A review

Label-Free Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy

Investigation of Gold Nanoparticle Naproxen-Derived Conjugations in Ovarian Cancer

Contact Info

Product

Resources

About