Investigation of magnesium–zinc–calcium alloys and bone marrow derived mesenchymal stem cell response in direct culture

Cipriano, Aaron F.; Sallee, Amy; Guan, Renguo; Zhao, Zhihao; Tayoba, Myla; Sanchez, Jorge de Jesus; Liu, Huinan

doi:10.1016/j.actbio.2014.10.018

Cited by 75 publications

(153 citation statements)

References 69 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…This causes a marked increase in local pH [16,63,[67][68][69] and locally high osmolality due to the release of ions into the medium [62,67]. The initially rapid evolution of hydrogen leads to gas cavitation and dramatically hinders cell attachment [69][70][71][72][73]; it also promotes the shedding of the surface degradation layer impeding the adhesion of cells, making it difficult to carry out cell culture experiments [27,67].…”

Section: Direct Culture Assaysmentioning

confidence: 99%

“…However, these available standards do not consider the physiological clearance of the ions released from the implanted Mg-based biomaterials by circulation in vivo [64]. The standards not only neglect the impact of the different accumulated ions in the extracts on the cytotoxicity results [62,81,82,95,96], the accumulation also contributes to the difference and even contradictive results of in vitro and in vivo studies [12,86,87,97]. There are two feasible approaches to reduce the accumulation of Mg and alloy ions in the extracts.…”

Section: Indirect Culture Assaysmentioning

confidence: 99%

“…The direct culture assays were introduced as part of a field-wide effort to improve and standardize the in vitro testing of Mg-based biomaterials. They are applied to simulate cellular processes at the implant site, to probe the cell-biomaterial interface directly [62] and to provide a more comprehensive in vitro method for the initial rapid screening of cytocompatibility and degradation of Mg-based materials than the ISO 10993-based methods [62][63][64][65].…”

Section: Direct Culture Assaysmentioning

confidence: 99%

“…The direct culture assays were established to investigate cytocompatibility at the dynamic surface of biodegradable Mg alloys, such as cardiovascular stents [62][63][64][65], that is, cells in direct contact with the samples. Nevertheless, it is difficult to identify the factors causing a reduction of cell viability and adhesion.…”

Section: Limitations Of Direct and Indirect Culture Assaysmentioning

confidence: 99%

See 3 more Smart Citations

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

et al. 2018

View full text Add to dashboard Cite

Biodegradable magnesium (Mg)-based vascular stents have been designed as temporary scaffolds to treat angiostenotic lesions for the maintenance of normal blood flow. Numerous studies have presented in vitro and in vivo tests for the evaluation of the safety and feasibility of Mg-based vascular stents and the related materials. Therein the cytocompatibility is a basic and important parameter in the evaluation system. In this review, we summarize the applications and limitations of in vitro evaluation methods including basic characterization methods and direct and indirect cytotoxicity tests. We discuss the influencing factors on cytotoxicity, such as surface roughness, preconditioning of sample surface, cell type for the biocompatibility evaluation in direct contact as well as conditions for the formation of extracts/ degradation products for indirect assays. Besides, we highlight the recent in vivo animal tests and clinical trials about Mgbased stents along with some associated results. The aim of this review is to provide a meaningful reference in the further developments and related evaluation methods of Mg-based stents.

show abstract

Section: Direct Culture Assaysmentioning

confidence: 99%

Section: Indirect Culture Assaysmentioning

confidence: 99%

Section: Direct Culture Assaysmentioning

confidence: 99%

Section: Limitations Of Direct and Indirect Culture Assaysmentioning

confidence: 99%

See 2 more Smart Citations

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Prevalently, Mg and coating strategies were tested concerning their degradation rate in vitro. Few studies addressed the biological outcome by extraction testing or exposing cells to the material for a short culture period only [31][32][33][34]. We wanted to examine the in vitro performance of the n-HA coating in a biological milieu as well as to reconsider if a direct in vitro test set up is suitable for evaluating highly degradable materials in extended culture times.…”

mentioning

confidence: 99%

Testing the in vitro performance of hydroxyapatite coated magnesium (AZ91D) and titanium concerning cell adhesion and osteogenic differentiation

Kleinhans¹,

Vacun

Surmenev³

et al. 2015

BioNanoMaterials

View full text Add to dashboard Cite

In the current study the in vitro outcome of a degradable magnesium alloy (AZ91D) and standard titanium modified by nanostructured-hydroxyapatite (n-HA) coatings concerning cell adhesion and osteogenic differentiation was investigated by direct cell culture. The n-HA modification was prepared via radiofrequency magnetron sputtering deposition and proven by field emission scanning electron microscopy and X-ray powder diffraction patterns revealing a homogenous surface coating. Human mesenchymal stem cell (hMSCs) adhesion was examined after one and 14 days displaying an enhanced initial cell adhesion on the n-HA modified samples. The osteogenic lineage commitment of the cells was determined by alkaline phosphatase (ALP) quantification. On day one n-HA coated AZ91D exhibited a comparable ALP expression to standard tissue culture polystyrene samples. However, after 14 days solely little DNA and ALP amounts were measurable on n-HA coated AZ91D due to the lack of adherent cells.Titanium displayed excellent cell adhesion properties and ALP was detectable after 14 days. An increased pH of the culture was measured for AZ91D as well as for n-HA coated AZ91D. We conclude that n-HA modification improves initial cell attachment on AZ91D within the first 24 h. However, the effect does not persist for 14 days in in vitro conditions.

show abstract

Hierarchical Therapeutic Ion‐Based Microspheres with Precise Ratio‐Controlled Delivery as Microscaffolds for In Situ Vascularized Bone Regeneration

Wan

Yuan

et al. 2022

Adv Funct Materials

View full text Add to dashboard Cite

Therapeutic ions, such as Si and Mg, play vital roles in regulating metabolism and promoting tissue repair, hence providing an efficient strategy in tissue engineering. The regenerative outcome is strongly dependent on the targeted delivery and controlled release of therapeutic ions. Nevertheless, it remains challenging to deliver multiple ions at controlled manners and ratios. Herein, hierarchical therapeutic ion‐based microspheres are fabricated. The coordinated release of Mg and Si ions at pre‐set ratios is achieved, based on which, Mg favors osteogenesis by inducing highly efficient cell recruitment and angiogenesis, and Si promotes massive collagen secretion and biomineralization to accelerate the bone maturation process. These therapeutic ion‐based microspheres (namely PNM2) can steadily release Mg and Si ions at an optimized ratio of 2:1, which shows the most significant synergistic effect on angiogenesis and osteogenesis. Furthermore, in a rat calvarial defects model, the volume and maturity of the vascularized neo‐bone tissue regenerated with PNM2 microspheres are comparable with or even surpassing those defects regenerated with growth factors and/or cell‐laden scaffolds. Overall, this platform provides a controllable strategy for the coordinated delivery of Mg/Si ions, opening a new avenue for developing therapeutic ion‐based microscaffold for tissue engineering.

show abstract

Investigation of magnesium–zinc–calcium alloys and bone marrow derived mesenchymal stem cell response in direct culture

Cited by 75 publications

References 69 publications

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

Testing the in vitro performance of hydroxyapatite coated magnesium (AZ91D) and titanium concerning cell adhesion and osteogenic differentiation

Hierarchical Therapeutic Ion‐Based Microspheres with Precise Ratio‐Controlled Delivery as Microscaffolds for In Situ Vascularized Bone Regeneration

Contact Info

Product

Resources

About