Investigation of lipolytic activity of the red king crab hepatopancreas homogenate by NMR spectroscopy

Abstract

The digestive gland of craboids (hepatopancreas) is rich in a huge number of various enzymes (collagenases, nucleases, hyaluronidases, proteases), which are well studied at the moment. However, little is known about crustacean lipases. In this work, using 1H NMR spectroscopy, it was found that the hepatopancreas homogenate of the red king crab Paralithodes camtschaticus demonstrates high lipolytic activity against triacetin in a wide pH range and shows moderate activity against the caprylic/capric triglyceride emulsion. Under the action of the hepatopancreas homogenate, triacylglycerols are converted into 1,2-diacylglycerol, and then into 2-monoacylglycerol and 1-monoacylglycerol. The 1-monoacylglycerol predominates in the reaction products. The use of NMR spectroscopy makes it possible to quickly detect hydrolysis products and evaluate the reaction direction.

Crabs Eriocheir japonica and Paralithodes camtschaticus Are a Rich Source of Lipid Molecular Species with High Nutritional Value

“…Authors associated it with the action of phospholipases, but they could not identify any aspects of handling and processing that would produce the elevated values [ 28 ]. Lipids were extracted simultaneously from all our samples, and the hepatopancreas samples did not contain DG and MAG in the lipid extract despite the presence of active lipases [ 29 ]. Further investigation is needed to explain the role of the high DG content in crab muscles.…”

Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab

“…The individual studies devoted to newly discovered enzymes from the hepatopancreas of the red king crab demonstrate that the crab fishery's waste byproduct, the hepatopancreas, is a source of valuable enzymes with high activity and a wide range of action. These enzymes possess unique properties distinct from previously described enzymes of other crustaceans [9,10]. The hepatopancreas of the red king crab can also be considered as a source of new AMPs, which could become prototypes of new candidates for broad-spectrum antibiotics and take a step towards solving the problem of antibiotic resistance, which poses a serious global threat.…”