Investigation of Lactobacillus paracasei encapsulation in electrospun fibers of Eudragit® L100

Abstract

Some species of Lactobacillus have demonstrated beneficial health effects being applied in the production of food supplements. Thus, the incorporation of viable cells as encapsulated probiotics represents an essential condition to be considered in new strategies for the controlled release of microorganisms. Herein, the massive encapsulation of Lactobacillus paracasei is provided by the use of alternative electrospinning technique. Is spite of the high voltage required for the production of fibers, a high density of viable cells is observed into the polymeric electrospun web, allowing the controlled release at targeted pH (characteristic of Eudragit® L100 polymer support). The reported procedure circumvents typical drawbacks of degradation of microorganisms under adverse conditions (storage, package and low pH) and preserves its biologic action after complete release from polymer fibers.

Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

“…Lactobacillus bacteria have also been incorporated into electrospun nanofibers, with L. plantarum being the most frequently used [24][25][26][27][28]. Other Lactobacillus species have also been electrospun using different polymers, namely, agrowaste amended with poly (vinyl alcohol) for Lactobacillus acidophilus [29], poly (vinyl alcohol) and sodium alginate for Lactobacillus rhamnosus [30], poly (vinyl alcohol) for Lactobacillus gasseri [31], and Eudragit L100 and sodium alginate for Lactobacillus paracasei [32]. In a recent study, we successfully incorporated nine different Lactobacillus species into polyethylene oxide (PEO) nanofibers (i.e., L. acidophilus, L. delbrueckii ssp.…”

Encapsulation of Pharmaceutical and Nutraceutical Active Ingredients Using Electrospinning Processes

“…In contrast, single layer CS alone did not provide benefits against simulated gastric fluid and bile insults in vitro [121]. Overall, it is clear that electrospinning circumvents the common drawbacks of probiotic degradation within a formulation, and preserves biological action after complete release from polymer fiber [122]. While some studies suggest that simple blend electrospinning is sufficient to provide these advantages, others indicate that a coaxial or multilayer is more effective at protecting the incorporated probiotics.…”

Research advances on encapsulation of probiotics with nanomaterials and their repair mechanisms on intestinal barriers

“…When using Eudragit ® L100 (Methacrylic acid, a film forming material used for oral tablets and capsules) and sodium alginate to encapsulate L. paracasei by electrospinning, the Eudragit L100 electrospun fibers can provide a hydrophobic environment for L. paracasei, protecting them from oxygen to preserve their viability [60]. L. acidophilus wrapped in nanofibers made from gum arabic and polyvinyl alcohol remained 63.99% of survival rate after lying in simulated gastrointestinal tract for 2 hours, while all free ones were dead [61].…”