Investigation of ischemia damaging action on blood serum by Raman spectroscopy method

Abstract

Raman spectroscopy method is applied for analysis of ischemia damaging action on serum. The registered changes of low-density lipoprotein (LDL) after brain ischemia (size increasing, density reducing) are explained in frameworks of free radical theory of oxidative stress. We confirmed by Raman spectroscopy method our hypothesis, according to which the increase in LDL size and decrease of LDL density after an ischemia, in contrast to LDL of healthy animals, is caused by loosing of LDL phospholipid monolayer under damaging action of free radicals. The obtained results show the possibility of using of Raman spectroscopy method for an estimation of ischemic damages. Averaged Raman spectrum of blood serum of healthy animals and animals after ischemia

Investigation of CsCl-induced aggregation of serum albumin depending on pH by Raman spectroscopy method

“…at pH 5.0. In process of moving from the isoelectric point, both in the direction of pH increase, and in the direction of pH decrease, the degree of complex formation of protein molecules decreases, that confirms our results obtained by the laser correlation spectroscopy method of elastic light scattering [1]. The cause of protein complex formation at the presence of heavy metal salt consists in following: the attraction of albumin macromolecules in absence of CsCl is precluded by their mutual Coulomb repulsion, and at addition of CsCl the cesium ions (on a surface of which one the water will not be retained owing to a low coupling of water with ions of heavy alkaline metals) can form electrostatic pairs with the appropriate charges on protein, completely compensating a surface charge of albumin.…”

“…Our researches [1] of aggregation of protein molecules at the presence of cesium chloride by dynamic light scattering method have shown, firstly, that size of protein aggregates increases directly proportional to concentrations of this salt, and secondly, that within the limits of each value of concentration of CsCl the relation of the size of albumin complexes to pH of buffer solution has a parabolic view with a maximum in isoelectric point of protein (pI 4.7), therefore, the strongest albumin aggregation takes place near to its isoelectric point. In this paper we have received confirmation of our results by Raman spectroscopy method.…”

“…In our previous researches on aggregation of serum albumin molecules induced by heavy metals salts (CsCl), we have used laser correlation spectroscopy methods of elastic light scattering (dynamic light scattering) [1]; in this work the method of Raman light scattering is applied for analysis of this phenomenon. The Raman light scattering, widely used for research of functions and structure of biological molecules, has a great potential for application in biological and medical science areas.…”

Research of protective action of ischemic preconditioning on components of blood serum at a brain ischemia by Raman spectroscopy method

“…In our previous researches of brain ischemia damaging action on components of blood serum we have used as methods of laser spectroscopy of elastically scattered light [4], so method of Raman spectroscopy [5].…”

Spectral-luminescent manifestation of the damaging action of brain ischemia on blood serum components

“…Luminescence spectra of rhodamine 6G (60 µM) in samples of a healthy blood serum (1) and blood serum after ischemia(2).…”