Investigation of interactions between the marine GY785 exopolysaccharide and transforming growth factor-β1 by atomic force microscopy

Zykwinska, Agata; Marquis, Mélanie; Sinquin, Corinne; Marchand, Laëtitia; Colliec-Jouault, Sylvia; Cuenot, Stéphane

doi:10.1016/j.carbpol.2018.08.104

Cited by 9 publications

(11 citation statements)

References 42 publications

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“…The release study has revealed that the majority of growth factor initially loaded in the microparticles was retained in both microcarriers, whatever the buffer used. As recently shown by AFM experiments, although TGF-β1 strongly interacts with the highly sulphated derivative, GY785 DRS and tends to form the nanoassemblies, the protein affinity toward a slightly sulphated derivative, GY785 DR, was also demonstrated [38]. Thus, the low growth factor release results most likely from these non-covalent polysaccharide-growth factor interactions, which efficiently retain the growth factor inside the microparticles.…”

Section: Resultsmentioning

confidence: 87%

“…In a previous study, GY785 DRS was shown to stimulate the hASC chondrogenic differentiation in vitro in the presence of TGF-β1, probably through non-covalent interactions with the growth factor [28]. Upon incubation, positively charged TGF-β1 (pI ~ 8.8) and negatively charged GY785 DRS were recently shown to spontaneously co-assemble and form nanoparticles [38]. This tight association between both entities may preserve the growth factor from proteolytic degradation, thus enhancing its bioactivity and its lifespan.…”

Section: Resultsmentioning

confidence: 99%

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Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

et al. 2019

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Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating Transforming Growth Factor-β1 (TGF-β1) into microparticles based on marine exopolysaccharide (EPS), namely GY785 EPS, for further applications in cartilage engineering. Using a capillary microfluidic approach, two microcarriers were prepared. The growth factor was either encapsulated directly within the microparticles based on slightly sulphated derivative or complexed firstly with the highly sulphated derivative before being incorporated within the microparticles. TGF-β1 release, studied under in vitro model conditions, revealed that the majority of the growth factor was retained inside the microparticles. Bioactivity of released TGF-β1 was particularly enhanced in the presence of highly sulphated derivative. It comes out from this study that GY785 EPS based microcarriers may constitute TGF-β1 reservoirs spatially retaining the growth factor for a variety of tissue engineering applications and in particular cartilage regeneration, where the growth factor needs to remain in the target location long enough to induce robust regenerative responses.

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

et al. 2019

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“…AFM has been used to explore the interaction between marine bacterial GY785 exopolysaccharide and transforming growth factor-β1, showing that the interaction can promote the differentiation of some tissues. The interaction was greatest between GY785 exopolysaccharide derivatives with higher vulcanization degree, indicating the important role of sulfate groups in protein binding (Zykwinska et al, 2018).…”

Section: Interaction Phenomena and Complexesmentioning

confidence: 96%

Investigation of food microstructure and texture using atomic force microscopy: A review

Wen

Liu

et al. 2020

Comp Rev Food Sci Food Safe

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We review recent applications of atomic force microscopy (AFM) to characterize microstructural and textural properties of food materials. Based on interaction between probe and sample, AFM can image in three dimensions with nanoscale resolution especially in the vertical orientation. When the scanning probe is used as an indenter, mechanical features such as stiffness and elasticity can be analyzed. The linkage between structure and texture can thus be elucidated, providing the basis for many further future applications of AFM. Microstructure of simple systems such as polysaccharides, proteins, or lipids separately, as characterized by AFM, is discussed. Interaction of component mixtures gives rise to novel properties in complex food systems due to development of structure. AFM has been used to explore the morphological characteristics of such complexes and to investigate the effect of such characteristics on properties. Based on insights from such investigations, development of food products and manufacturing can be facilitated. Mechanical analysis is often carried out to evaluate the suitability of natural or artificial materials in food formulations. The textural properties of cellular tissues, food colloids, and biodegradable films can all be explored at nanometer scale, leading to the potential to connect texture to this fine structural level. More profound understanding of natural food materials will enable new classes of fabricated food products to be developed.

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“…However, a physical Ca 2+ gel was formed by a medium molecular weight (MMW) derivative, EPS DR (240,000 g/mol), obtained by radical depolymerization of the native HMW EPS (Zykwinska et al, 2019;Gélébart et al, 2022). Besides gelling properties with divalent cations, the negatively charged EPS is also able to bind to positively charged proteins, such as basic growth factors, and this property has a huge potential in tissue engineering (Merceron et al, 2012;Rederstorff et al, 2017;Zykwinska et al, 2018;Zykwinska et al, 2019;Gélébart et al, 2022). Indeed, growth factors constitute a key element in tissue engineering strategies since they stimulate essential cellular processes (proliferation, migration, differentiation).…”

Section: Introductionmentioning

confidence: 99%

Interactions between infernan and calcium: From the molecular level to the mechanical properties of microgels

Zykwinska

Makshakova

Gélébart

et al. 2022

Carbohydrate Polymers

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Investigation of interactions between the marine GY785 exopolysaccharide and transforming growth factor-β1 by atomic force microscopy

Cited by 9 publications

References 42 publications

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

Investigation of food microstructure and texture using atomic force microscopy: A review

Interactions between infernan and calcium: From the molecular level to the mechanical properties of microgels

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