Investigation of Instrument Parameters for the Application of Peroxyoxalate Cheminescence Detection to Microbore Chromatography

Grayeski, Mary Lynn; Weber, Andrew J.

doi:10.1080/00032718408065328

Cited by 32 publications

(7 citation statements)

References 10 publications

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“…4a, the W h/2 for luminol and ABEI was 16.8 and 20.2 s. It was better than that obtained by oncolumn UV detection with the W h/2 of 23.1 s for luminol and 38.9 s for ABEI. The result of comparison validated an actual decrease in the peak width occurred when the CL interface was used, which has been previously observed and can be explained in terms of the "chemical band narrowing" effect [16,31,32]. It is well known that there was no need for extra flow cell in UV detection, and the poor column efficiency obtained by cHPLC-UV possibly resulted from serious axial diffusion of the solutes in the column due to the low flow rate propelled by pump.…”

Section: Comparison Of Band Broadening With Uv Detection and CL Detecmentioning

confidence: 60%

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Evaluation of band broadening in chemiluminescence detection coupled to pressurized capillary electrochromatography with an off‐column coaxial flow interface

Lin

et al. 2008

Electrophoresis

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A system of off-column coaxial flow chemiluminescence (CL) detection coupled to pressurized CEC (pCEC) was described. The interface utilized a reactor that introduced postcolumn CL reagent into the capillary effluents in a sheathing flow profile. To compare and evaluate band broadening of analytes caused by the detector, the typical CL compounds luminol and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) were separated and detected by pCEC or capillary HPLC (cHPLC) coupled to CL and UV detector, respectively. The results demonstrated that the band broadening caused by off-column detection interface was minimized due to the fast kinetic nature of the CL reaction. With the proposed pCEC-CL system, the detection limits of luminol and ABEI were 1.0x10(-8) and 8.0x10(-8) mol/L, respectively, which were approximately 100-fold more sensitive than those obtained with UV absorption. In addition, separation and detection of the ABEI-labeled L-lysine (L-Lys) and L-arginine (L-Arg) were accomplished by pCEC-CL method based on the principle of ABEI-potassium ferricyanide-alkaline medium CL reaction system. Under the optimum conditions, good results could be achieved compared with pCEC-UV.

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Section: Comparison Of Band Broadening With Uv Detection and CL Detecmentioning

confidence: 60%

“…In this mode, however, band broadening become an important influencing factor. Owing to the "chemical band narrowing" effect [31,32] in CL detection, a decline in a relative chromatographic band broadening could be expected as compared to UV detection, which made it possible for acquiring a better resolution [16,33].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of band broadening in chemiluminescence detection coupled to pressurized capillary electrochromatography with an off‐column coaxial flow interface

Lin

et al. 2008

Electrophoresis

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“…The decrease in peak width has been previously observed and can be explained in terms of the "chemical band narrowing" effect. [26][27][28] The CL reaction kinetics allow for an intense, rapidly decaying signal to be produced when the metal ions enter the reaction zone of the interface. Unlike conventional photometric detectors, a signal is produced only when the analyte is in contact with "fresh" reagents.…”

Section: Resultsmentioning

confidence: 99%

On-Line Chemiluminescence Detection for Capillary Ion Analysis

et al. 1996

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Highly sensitive chemiluminescence detection for capillary ion analysis has been developed. The new idea lies in using the chemiluminescence reagent luminol as a component of the separation electrolyte, thus preventing loss of the light signal where luminol and H 2 O 2 are mixed in advance in conventional flow injection analysis or ion chromatography chemiluminescence detection. Signal enhancement is achieved by sample stacking injection in the electrophoresis process. The detection limit (S/N ) 3) of 20 zmol (5 × 10 -13 mol/L) for cobalt(II) is ∼2 orders of magnitude better than the most sensitive results (1.7 × 10 -11 mol/L) reported so far. Detection limits are 2 amol, 80 amol, 740 amol, and 100 fmol for copper(II), nickel(II), iron(III), and manganese(II), respectively. The sensitivity is significantly better than that in the electrochemical detection and the commonly used UV detection in capillary ion analysis. The capillary electrophoresischemiluminescence detector has been used to separate five metal ions, cobalt(II), copper(II), nickel(II), iron(III), and manganese(II), within 8 min with an average theoretical plate number of 4.6 × 10 5 .

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“…The detection sensitivities for several fluorophores are one or two orders higher by PO-CL detection than by fluorescence (FL) detection. In the field of HPLC, the method has been applied to the determination of femtomole or attomole levels of polycyclic aromatic hydrocarbons (PAHs) (Sigvardson and Birks, 1983; Sigvardson el al., 1984; Grayeski and Weber, 1984;Weinberger et al, 1984), dansylamines (Mellbin, 1983; De Jong et al, 1984; Miyaguchi et al, 1984; Mellbin and Smith, 1984; Koziol et al, 1984;Miyaguchi et al, 1986;Nozaki et al, 1988; Hayakawa et al, 1989), cyanobenzoisoindoleamines (Kawasaki el al., 1990) and other compounds. These experiments, however, required expensive pulseless pumps of the cylinder-type or double plungertype and highly efficient dampers to reduce the background noise and improve the sensitivity.…”

Section: Introductionmentioning

confidence: 95%

Effect of a dual‐head short‐stroke pump on post‐column peroxyoxalate chemiluminescence detection

Hayakawa

Imaizumi

Miyazaki

1991

Biomedical Chromatography

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A dual-head short-stroke pump has two advantages in the post-column peroxyoxalate chemiluminescence (PO-CL) detection system. The first is to increase the mixing efficiency of solutions. The second is to increase the stability of the PO-CL reaction by keeping the aryl oxalate and hydrogen peroxide solutions separate. The detection sensitivities for six polycyclic aromatic hydrocarbons (PAHs) increased in the present system by using bis(2,4-dinitrophenyl) oxalate (DNPO) or bis(2,3,4,5,6-pentafluorophenyl) oxalate (PFPO) instead of such popular aryl oxalates as bis(2,4,6-trichlorophenyl) oxalate (TCPO) and bis[2-(3,6,9-trioxadecyloxycarbonyl)-4-nitrophenyl] oxalate (TDPO). Both DNPO and PFPO increased the sensitivities by factors of 4.1-10.2 and 3.5-8.1, respectively. In addition, DNPO was more stable than PFPO in acetonitrile. These results suggest that DNPO is the most useful aryl oxalate for the sensitive PO-CL detection of PAHs.

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Investigation of Instrument Parameters for the Application of Peroxyoxalate Cheminescence Detection to Microbore Chromatography

Cited by 32 publications

References 10 publications

Evaluation of band broadening in chemiluminescence detection coupled to pressurized capillary electrochromatography with an off‐column coaxial flow interface

Evaluation of band broadening in chemiluminescence detection coupled to pressurized capillary electrochromatography with an off‐column coaxial flow interface

On-Line Chemiluminescence Detection for Capillary Ion Analysis

Effect of a dual‐head short‐stroke pump on post‐column peroxyoxalate chemiluminescence detection

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