Investigation of induction effect on the steady state performance of a continuous fermentation for recombinant β-ga1actosidase

Strategies for producing recombinant sucrose phosphorylase originating from Bifidobacterium longum in Escherichia coli JM109

“…Also, in M9 media, glucose was better for cell growth than sucrose or the complex of glucose and sucrose. For both LB and M9 media, most of the cultivations with added IPTG resulted in higher cell growth compared to those without IPTG, which is in opposition to other reports where lower cell growth was shown with the addition of IPTG [31][32][33].…”

Facile electrochemical detection of Escherichia coli using redox cycling of the product generated by the intracellular β-d-galactosidase

“…The method is based on the determination of Gal activity inside E. coli, and it employs ECC redox cycling along with the IPTG and permeabilization treatments [31,32]. First, the effects of IPTG and permeabilization treatments were investigated, and the conditions such as the pH of the incubation solution, the IPTG concentration, and the incubation period were optimized.…”