Investigation of in vivo antioxidant property of Abelmoschus esculentus (L) moench. fruit seed and peel powders in streptozotocin-induced diabetic rats

Sabitha, V.; Ramachandran, Subramaniam; Naveen, Koikaramparambil Robert; Panneerselvam, Kaliyamoorthy

doi:10.4103/0975-9476.104432

Cited by 40 publications

(18 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The reduced levels of antioxidant enzymes in the pancreas seen here are consistent with previously reports examining NOD mice (22), and diabetic rats induced by streptozotocin (23). …”

Section: Discussionsupporting

confidence: 92%

Epigallocatechin-3-gallate modulates anti-oxidant defense enzyme expression in murine submandibular and pancreatic exocrine gland cells and human HSG cells

Dickinson

DeRossi²,

et al. 2014

Autoimmunity

View full text Add to dashboard Cite

Sjogren’s syndrome (SS) and type 1 diabetes are prevalent autoimmune diseases in the United States. We reported previously that Epigallocatechin-3-gallate (EGCG) prevented and delayed the onset of autoimmune disease in NOD mice, a model for both Sjogren’s syndrome (SS) and type 1 diabetes. EGCG also normalized the levels of proteins related to DNA repair and antioxidant activity in NOD.B10.Sn-H2 mice, a model for primary SS, prior to disease onset. The current study examined the effect of EGCG on the expression of antioxidant enzymes in the submandibular salivary gland and the pancreas of NOD mice and cultured human salivary gland acinar cells. NOD mice consuming 0.2% EGCG daily dissolved in water showed higher protein levels of peroxiredoxin 6 (PRDX6), a major antioxidant defense protein, and catalase, while the untreated NOD mice exhibited significantly lowered levels of PRDX6. Similarly, pancreas samples from water-fed NOD mice were depleted in PRDX6 and superoxide dismutase, while EGCG-fed mice showed high levels of these antioxidant enzymes. In cultured HSG cells EGCG increased PRDX6 levels significantly, and this was inhibited by p38 and JNK inhibitors, suggesting the EGCG-mediated increase in protective antioxidant capacity is regulated in part through MAPK pathway signaling. This mechanism may explain the higher levels of PRDX6 found in EGCG-fed NOD mice. These preclinical observations warrant future preclinical and clinical studies to determine whether EGCG or green tea polyphenols could be used in novel preventive and therapeutic approaches against autoimmune diseases and salivary dysfunction involving oxidative stress.

show abstract

“…The reduced levels of antioxidant enzymes in the pancreas seen here are consistent with previously reports examining NOD mice (22), and diabetic rats induced by streptozotocin (23). …”

Section: Discussionsupporting

confidence: 92%

Epigallocatechin-3-gallate modulates anti-oxidant defense enzyme expression in murine submandibular and pancreatic exocrine gland cells and human HSG cells

Dickinson

DeRossi²,

et al. 2014

Autoimmunity

View full text Add to dashboard Cite

show abstract

“…A previous work of Sabitha et al revealed that AE reduced blood glucose and lipids, and increased body weight in streptozotocin (STZ)-induced diabetic rats [ 4 ]. Possessing a good anti-oxidation ability, AE has been shown to decrease lipid peroxidation, increase the levels of superoxide dismutase, catalase, and glutathione peroxidase, and the reduced glutathione in the liver, kidney and pancreas of diabetic rats [ 5 ]. However, in these reports, the experimental animals were fed with AE powder of the seeds and peel which was crude, preventing the bioactive components from being identified.…”

Section: Introductionmentioning

confidence: 99%

The nutraceutical benefits of subfractions of Abelmoschus esculentus in treating type 2 diabetes mellitus

et al. 2017

View full text Add to dashboard Cite

Abelmoschus esculentus (AE), a commonly consumed vegetable, is well-known for its anti-hyperglycemic effects. However, few scientific reports have identified its targets because mucilage increases the difficulty of manipulation. We recently reported extraction steps to obtain subfractions of AE, which were found to attenuate the adverse effects of high glucose and fatty acid in vitro. In this study, we used modified extraction steps and type 2 diabetic rats to explore whether AE subfractions can improve the metabolic disturbances caused by insulin resistance in vivo. AE subfractions (F1, F2, and FR) were prepared. The type 2 diabetes model was induced by feeding male Sprague-Dawley rats with a high-fat diet and injecting them with 35 mg/kgbw streptozotocin when their body weight reached 475 ± 15 g. After a hyperglycemic status had been confirmed, the rats were tube-fed with or without different doses of AE subfractions. Serum glucose, lipid markers, insulin, HbA1c and HOMA-IR were measured in the following 12 weeks. Serum glucose promptly increased and insulin resistance was noted in the diabetic rats (glucose: 360–500 mg/dl, HOMA-IR 9.8–13.8). F2, rich in polysaccharides and carbohydrates, was most effective in attenuating hyperglycemia and insulin resistance (glucose: 200 mg/dl; HOMA-IR: 5.3) and especially HbA1C (from 8.0% to 6.5%). All of the AE subfractions lowered the level of triglycerides and free fatty acid, but not the level of total cholesterol. FR significantly increased the high-density lipoprotein/low-density lipoprotein ratio, indicating its benefits for lipoprotein profiles. While F2 and FR were associated with weight gain, F1 possessed an anti-obese effect. In conclusion, whether it is consumed as a vegetable or as a nutraceutical, AE has the potential to be an adjuvant therapy for diabetes. AE subfractions could be developed individually and deserve further investigation.

show abstract

“…Okra polysaccharide, which consists of rhamnose, arabinose, galactose, glucuronic acid, and galactosyl acid, also reduced pro-inflammatory markers, including mRNA expressions of TLR4, NF-κB p65 and IKKα, and protein expressions of TNFα, IL-6, and IL-1β in the chronic stress-induced mice hippocampus, and upregulated ERK1/2, JNK, and p38 (MAPK signaling) in Bv-2 microglia cells [ 42 ]. Furthermore, aqueous extract of okra seed, which mainly consists of catechin and quertin derivatives, reduced MDA, and increased antioxidant activities, and elevated neurotransmitter levels such as dopamine, norepinephrine, acetylcholine, serotonin, and epinephrine in the hippocampus of depressed mice [ 43 ]. Based on existing literature, insulin/PI3K/Akt/Tau/Bax [ 14 , 15 ], microglia-driven neuroinflammatory [ 57 ], and oxidative stress-neurogenesis [ 10 ] pathways in the hippocampus were associated with HFD-induced neuronal injury, which are coinciding with the neuroprotective property of okra against non-HFD-model-induced neuronal injury in the hippocampus.…”

Section: Discussionmentioning

confidence: 99%

Abelmoschus Esculentus (L.) Moench’s Peel Powder Improves High-Fat-Diet-Induced Cognitive Impairment in C57BL/6J Mice

Prom-in

Kaewsrichan

Wangpradit

et al. 2020

IJERPH

View full text Add to dashboard Cite

Okra peel exhibits numerous therapeutic effects. This study explores the potential ameliorative effects of okra peel powder on high-fat-diet (HFD)-induced hypercholesterolemia and cognitive deficits. Thirty-six C57BL/6J male mice were randomly divided into six groups (n = 6 per group): (i) control, mice fed with a normal diet; (ii) HFD, mice fed with HFD; (iii) HFD-SIM, mice fed with HFD and given simvastatin (20 mg/kg/day); (iv) HFD-OP1; (v) HFD-OP2; (vi) HFD-OP3, mice fed with HFD and okra peel (200, 400, or 800 mg/kg/day, respectively). Following 10 weeks of treatments, the mice were subjected to the Morris water maze (MWM). Parameters such as weekly average body weight, food intake, and blood lipid profiles were also recorded. The HFD group showed a profound increase in total cholesterol and low-density lipoprotein concentration compared to the control group. All okra-treated and HFD-SIM groups performed better than the HFD group during acquisition trials, whereas only the HFD-OP1 produced a significantly higher number of entries into the platform zone during the probe trial. In sum, all three okra doses improved the learning ability of the mice. However, only the lowest dose of okra significantly improved the spatial reference memory retention.

show abstract

Investigation of in vivo antioxidant property of Abelmoschus esculentus (L) moench. fruit seed and peel powders in streptozotocin-induced diabetic rats

Cited by 40 publications

References 22 publications

Epigallocatechin-3-gallate modulates anti-oxidant defense enzyme expression in murine submandibular and pancreatic exocrine gland cells and human HSG cells

Epigallocatechin-3-gallate modulates anti-oxidant defense enzyme expression in murine submandibular and pancreatic exocrine gland cells and human HSG cells

The nutraceutical benefits of subfractions of Abelmoschus esculentus in treating type 2 diabetes mellitus

Abelmoschus Esculentus (L.) Moench’s Peel Powder Improves High-Fat-Diet-Induced Cognitive Impairment in C57BL/6J Mice

Contact Info

Product

Resources

About