Investigation of in vivo anti-inflammatory and anti-angiogenic attributes of coumarin-rich ethanolic extract of Melilotus indicus

Saleem, Mohammad; Asif, Muhammad; Parveen, Anum; Yaseen, Hafiza Sidra; Saadullah, Malik; Bashir, Asiya; Asif, Jawaria; Arif, Mehwish; Khan, Ikram Ullah; Khan, Rizwan Ullah

doi:10.1007/s10787-020-00703-9

Cited by 15 publications

(14 citation statements)

References 27 publications

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“… 22 Coumarin has anti-inflammatory activity due to its high hydroxyl group scavenging capacity and redox action. 23 Therefore, in the present study, quercetin and coumarin, as reported in our previous study, 14 were found to be highest in concentration in M. indicus (L.) All. Ethanolic extract was also explored for its anticancer activity against DMBA-induced cancer.…”

Section: Discussionsupporting

confidence: 80%

Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies

et al. 2022

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Melilotus indicus (L.) All. is known to have anti-inflammatory and anticancer properties. The present study explored the in vivo skin carcinogenesis attenuating potential of ethanolic extract of M. indicus (L.) All. (Miet) in a 7,12-dimethylbenz[ a ]anthracene (DMBA)-induced skin cancer model. The ethanolic extract of the plant was prepared by a maceration method. HPLC analysis indicated the presence of quercetin in abundance and also various other phytoconstituents. DPPH radical scavenging assay results showed moderate antioxidant potential (IC 50 = 93.55 ± 5.59 μg/mL). A topical acute skin irritation study showed the nonirritant nature of Miet. Data for the skin carcinogenic model showed marked improvement in skin architecture in Miet and its primary phytochemicals (quercetin and coumarin) treated groups. Miet 50% showed comparable effects with 5-fluorouracil. Significant ( p < 0.05) anticancerous effects were seen in coumarin–quercetin combination-treated animals than in single agent (coumarin and quercetin alone)-treated animals. Chorioallantoic membrane (CAM) assay results showed the antiangiogenic potential of Miet. Treatment with Miet significantly down-regulated the serum levels of CEA (carcinoembryonic antigen) and TNF-α (Tumor necrosis factor-α). Data for the docking study indicated the binding potential of quercetin and coumarin with TNF-α, EGFR, VEGF, and BCL2 proteins. Thus, it is concluded that Miet has skin cancer attenuating potential that is proposed to be due to the synergistic actions of its bioactive molecules. Further studies to explore the effects of Miet and its bioactive molecules as an adjuvant therapy with low dose anticancer drugs are warranted, which may lead to a new area of research.

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Section: Discussionsupporting

confidence: 80%

Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies

et al. 2022

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“…As avian embryos are not considered to be laboratory animals during the first twothirds of embryonic development (Directive 2010/63/EU 2020), no ethics committee approval is required. Originally, the highly vascularized chorioallantoic membrane was employed as a model to study the basic mechanisms behind angiogenesis and wound healing (Ribatti 2016), and thus, it is frequently used to investigate the angiogenic and antiangiogenic effects of substances (Dar and Sehgal 2020;Saleem et al 2020). Because of its versatile applicability, the in ovo system has found application in areas such as drug development, cosmetics and food toxicology and therefore represents a suitable model to investigate the irritation potential and toxicity of substances and formulations.…”

Section: Toxicity Applications With Het-cammentioning

confidence: 99%

Alternative model organisms for toxicological fingerprinting of relevant parameters in food and nutrition

Sandner

König

Wallner

et al. 2021

Critical Reviews in Food Science and Nutrition

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In the field of (food) toxicology, there is a strong trend of replacing animal trials with alternative methods for the assessment of adverse health effects in humans. The replacement of animal trials is not only driven by ethical concerns but also by the number of potential testing substances (food additives, packaging material, contaminants, and toxicants), which is steadily increasing. In vitro 2D cell culture applications in combination with in silico modeling might provide an applicable first response. However, those systems lack accurate predictions of metabolic actions. Thus, alternative in vivo models could fill the gap between cell culture and animal trials. In this review, we highlight relevant studies in the field and spotlight the applicability of alternative models, including C. elegans, D. rerio, Drosophila, HET-CAM and Lab-on-a-chip.

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“…Moreover, chronic inflammation associated with bone joint destruction causes damage to the surface and extracellular matrix of articular cartilage, which results in bone erosion if untreated. It hampers the quality of life ( Saleem, Iftikhar, et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Bradykinin is the most active mediator of nociception. Its acts under the effect of prostaglandins and plays a role in spasmogenic action on uterine, bronchial and intestinal smooth muscles ( Saleem, Asif, et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Anti-Inflammatory and Anti-Angiogenic Aattributes of Moringa olifera Lam. and its Nanoclay-Based Pectin-Sericin films

Buabeid¹,

Yaseen

Asif

et al. 2022

Front. Pharmacol.

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Background: Inflammation is a strong reaction of the non-specific natural immune system that helps to start protective responses against encroaching pathogens and develop typical immunity against intruding factors. However, prolonged inflammation may lead to chronic autoimmune diseases. For thousands of years, medicinal plants have served as an excellent source of treatment for chronic pathologies such as metabolic diseases.Purpose: The present study aims to evaluate the anti-inflammatory and anti-angiogenic potential of Moringa olifera Lam. extract (MO) and Moringa-loaded nanoclay films.Methods: The extract preparation was done through the maceration technique using absolute methanol (99.7%) and labelled as Mo. Me. Mo. Me-loaded nanoclay-based films were prepared by using pectin and sericin (Table 1). The in vitro studies characterized the film thickness, moisture, and phytochemical contents. The in vivo anti-inflammatory tests involved using a cotton pellet-induced granuloma model assay. In addition, the chick chorioallantoic membrane (CAM) assay was employed for angiogenesis activity.Results: The phytochemical analysis of the extract confirmed the presence of alkaloids, glycosides, flavonoids and phytosterol. This extract contained quercetin in a large quantity. Cotton-pellet induced granuloma model study revealed a comparable (p > 0.05) effect of a high dose of Mo. Me (500 mg/kg) as compared with standard drug. Noteworthy, data obtained through the RT-PCR technique manifested the dose-dependent anti-oedematous effect of Moringa olifera via downregulation of TNF-α and interleukin-1ß. The findings of the CAM assay exhibited a remarkable anti-angiogenic activity of Mo. Me loaded nanoclay films, showing diffused vasculature network in the macroscopic snapshot.Conclusion:Moringa olifera and its nanocomposite films have therapeutic potential against inflammation.

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Investigation of in vivo anti-inflammatory and anti-angiogenic attributes of coumarin-rich ethanolic extract of Melilotus indicus

Cited by 15 publications

References 27 publications

Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies

Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies

Alternative model organisms for toxicological fingerprinting of relevant parameters in food and nutrition

Anti-Inflammatory and Anti-Angiogenic Aattributes of Moringa olifera Lam. and its Nanoclay-Based Pectin-Sericin films

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