Investigation of important biochemical compounds from selected freshwater macroalgae and their role in agriculture

Abstract

Background Freshwater macroalgae possess a number of important secondary metabolites. They are an unexplored source of medicinal compounds. In this study, three freshwater macroalgae—Chara vulgaris, Cladophora glomerata and Spirogyra crassa—were collected from the river Swat and the river Kabul in the Charsadda district of Khyber Pakhtunkhwa, Pakistan. To assess the role of freshwater macroalgae in agriculture, various experiments were performed on their extracts. Methanolic extract of the three macroalgae were first analyzed through gas chromatography–mass spectrometry (GC–MS) for the presence of important medicinal secondary metabolites. The methanol based macroalgae extracts were tested for antibacterial, insecticidal, cytotoxic and phytotoxic activities. Results Initially, the algae were dried, crushed and treated with methanol for the extraction of secondary metabolites. The GC–MS results contained several important long chain fatty acids and other related long-chain hydrocarbons, such as alkanes and alkenes. Several benzene derivatives were also detected during the course of the investigation. Several of these compounds have established roles in the treatment of human ailments and can be supplied to farm animals. For example, phenylephrine is a decongestant, dilates pupils, increases blood pressure and helps in relieving hemorrhoids. Hexahydropseudoionone has uses in perfumes and other cosmetics. Several essential oils were also detected in the methanolic extract of the three macroalgae that can be utilized in various industrial products. Bioassays showed that these algal extracts—especially the Spirogyra sp. extract—contain moderate to maximum bioactivity. Conclusions Macroalgae possess important secondary metabolites with medicinal properties. These secondary metabolites can be used as biopesticides, plant growth enhancers, and remedies for various diseases in farm animals and for the control of weeds. They can be further explored for isolation and purification of useful biochemical compounds. Graphical Abstract

Bioactive Zincated Urea Effectively Enhanced Crop Yield, Nitrogen Use Efficiency, and Grain Quality in Maize and Rice

“…Under the current scenario, coated fertilizers are believed to be the best solution to deliver nutrients to crops for enhancing their productivity, reducing nutrient losses, and mitigating the subsequent impact on the environment [48,49]. The coating technology is devised for the slow release of the nutritional content of fertilizers so that their release rate may be synchronized with the nutritional demand of the plants.…”

Prokinetic and Laxative Effects of Chrozophora tinctoria Whole Plant Extract

“…The sample was injected, 1 µL at a time, with an initial oven temperature of 50 °C that was maintained for 2 min followed by gradually elevating the temperature to 150 °C at a rate of 8 °C/min. Finally, the temperature was raised to 300 °C at a rate of 15 °C/min and maintained for 5 min [ 33 , 34 ].…”

Extraction and Fractionation of Prokinetic Phytochemicals from Chrozophora tinctoria and Their Bioactivities

“…The injection device was run in a split mode at 250 • C. The sample was injected, 1 µL at a time, with an initial oven temperature of 50 • C that was maintained for 2 min followed by gradually elevating the temperature to 150 • C at a rate of 8 • C/min. Ultimately, the temperature was increased to 300 • C at a speed of 15 • C/min and sustained for 5 min [34,35]. The mass spectrometry was performed in full scan mode to obtain information about mass fragments and mass/charge (m/z) ratio in the range of 50-600.…”