Investigation of immunomodulatory and anti-inflammatory effects of eriodictyol through its cellular anti-oxidant activity

Abstract

Many studies have been performed to assess the potential utility of natural products as immunomodulatory agents to enhance host responses against infection or to ameliorate immune-based pathologies. To determine whether eriodictyol has immunomodulatory effects and clarify which types of immune effector cells are stimulated in vitro, we investigated the stimulatory effect of eriodictyol on spleen cells isolated from BALB/c mice. Eriodictyol significantly stimulated splenocyte proliferation. However, only B lymphocytes (not T lymphocytes) could be stimulated by eriodictyol in a dose-related manner. Studies assessing potential effect of eriodictyol on innate immunity reported that eriodictyol enhanced significantly the killing activity of natural killer (NK) cells, T lymphocytes, and macrophages. We also demonstrated that eriodictyol inhibited nitric oxide (NO) production and lysosomal enzyme activity in murine peritoneal macrophages cultured ex-vivo, suggesting a potential anti-inflammatory effect in situ. Eriodictyol revealed also a cellular anti-oxidant activity in splenocytes and macrophages. Furthermore, eriodictyol increased catalase activity in spleen cells. From this data, it can be concluded that eriodictyol exhibited an immunomodulatory effect that could be ascribed in part to a cytoprotective effect related to its anti-oxidant activity.

A comprehensive study of eriocitrin metabolism in vivo and in vitro based on an efficient UHPLC-Q-TOF-MS/MS strategy

“…For example, M1 (N2, Q2), namely, eriodictyol, a natural avonoid compound present in citrus fruits, has been reported to have broad bioactivities such as antioxidant, anti-inammatory, immunomodulatory and antidiabetic activities. [35][36][37] It is worth mentioning that eriodictyol was found to be one of the most potent insulin secretagogues among hundreds of compounds tested. 38 M19b, namely, hesperidin, a type of citrus bioavonoid distributed in foods including grapefruits, oranges and lemons, has many pharmacological activities, such as antioxidant, anti-depression and antitumour activities.…”

Eriodictyol ameliorates lipopolysaccharide‐induced acute lung injury by suppressing the inflammatory COX‐2/NLRP3/NF‐κB pathway in mice

“…Eriodictyol is a polyphenol flavonoid widely distributed in fruits and vegetables, mainly found in lemons and peanuts . Previous studies have highlighted multiple pharmacological effects of eriodictyol, including anti‐inflammatory and antioxidant activities . However, few studies have been reported on the effect of eriodictyol on ALI and its mechanism.…”

“…[15] Previous studies have highlighted multiple pharmacological effects of eriodictyol, including anti-inflammatory and antioxidant activities. [16,17] However, few studies have been reported on the effect of eriodictyol on ALI and its mechanism. Therefore, in this study, we examined the protective effect of eriodictyol on ALI and explored its mechanism of action.…”

Search for Natural Compounds That Increase Apolipoprotein A‐I Transcription in HepG2 Cells: Specific Attention for BRD4 Inhibitors

“…The optimal dose of eriodictyol in our study was 50 μM, which has been reported as nontoxic in various cell lines, such as macrophage mouse cells, NIH3T3 (fibroblast) cells, and HaCaT (human skin) cells (Lee et al, ). In vivo studies in mice suggested that eriodictyol is antiinflammatory (Zhu et al, ), while in vitro studies in mouse spleen cells showed that this compound has antioxidant properties (Mokdad‐Bzeouich et al, ). In HepG2 cells, eriodictyol increased insulin‐stimulated glucose uptake (Zhang et al, ) and inhibited of c‐Jun N‐terminal kinase (JNK) in macrophages (Lee et al, ).…”