Investigation of Image Reconstruction Parameters of the Mediso nanoScan PC Small-Animal PET/CT Scanner for Two Different Positron Emitters Under NEMA NU 4-2008 Standards

Gaitanis, Anastasios; Kastis, George A.; Vlastou, Elena; Bouziotis, Penelope; Verginis, Panayotis; Anagnostopoulos, Constantinos

doi:10.1007/s11307-016-1035-9

Cited by 13 publications

(11 citation statements)

References 20 publications

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“…This is most probably due to the fact that preclinical PET/MR systems were introduced recently in comparison with single PET and PET/CT scanners. Most studies in the past have estimated the outcome of in vivo studies solely based on the performance of the PET in phantoms which does not always predict the performance in animals [ 14 , 18 , 29 – 31 ].…”

Section: Discussionmentioning

confidence: 99%

Performance of nanoScan PET/CT and PET/MR for quantitative imaging of 18F and 89Zr as compared with ex vivo biodistribution in tumor-bearing mice

et al. 2021

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Introduction The assessment of ex vivo biodistribution is the preferred method for quantification of radiotracers biodistribution in preclinical models, but is not in line with current ethics on animal research. PET imaging allows for noninvasive longitudinal evaluation of tracer distribution in the same animals, but systemic comparison with ex vivo biodistribution is lacking. Our aim was to evaluate the potential of preclinical PET imaging for accurate tracer quantification, especially in tumor models. Methods NEMA NU 4-2008 phantoms were filled with 11C, 68Ga, 18F, or 89Zr solutions and scanned in Mediso nanoPET/CT and PET/MR scanners until decay. N87 tumor-bearing mice were i.v. injected with either [18F]FDG (~ 14 MBq), kept 50 min under anesthesia followed by imaging for 20 min, or with [89Zr]Zr-DFO-NCS-trastuzumab (~ 5 MBq) and imaged 3 days post-injection for 45 min. After PET acquisition, animals were killed and organs of interest were collected and measured in a γ-counter to determine tracer uptake levels. PET data were reconstructed using TeraTomo reconstruction algorithm with attenuation and scatter correction and regions of interest were drawn using Vivoquant software. PET imaging and ex vivo biodistribution were compared using Bland–Altman plots. Results In phantoms, the highest recovery coefficient, thus the smallest partial volume effect, was obtained with 18F for both PET/CT and PET/MR. Recovery was slightly lower for 11C and 89Zr, while the lowest recovery was obtained with 68Ga in both scanners. In vivo, tumor uptake of the 18F- or 89Zr-labeled tracer proved to be similar irrespective whether quantified by either PET/CT and PET/MR or ex vivo biodistribution with average PET/ex vivo ratios of 0.8–0.9 and a deviation of 10% or less. Both methods appeared less congruent in the quantification of tracer uptake in healthy organs such as brain, kidney, and liver, and depended on the organ evaluated and the radionuclide used. Conclusions Our study suggests that PET quantification of 18F- and 89Zr-labeled tracers is reliable for the evaluation of tumor uptake in preclinical models and a valuable alternative technique for ex vivo biodistribution. However, PET and ex vivo quantification require fully described experimental and analytical procedures for reliability and reproducibility.

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Section: Discussionmentioning

confidence: 99%

Performance of nanoScan PET/CT and PET/MR for quantitative imaging of 18F and 89Zr as compared with ex vivo biodistribution in tumor-bearing mice

et al. 2021

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“…Several research groups [6][7][8][9][10][11] have published articles where they discussed about performance of preclinical PET scanner. However, there are no long-term performance monitoring, manufacturer reference values or acceptance test nor any description on how such evaluations were actually performed.…”

Section: Discussionmentioning

confidence: 99%

Quality control of small animal PET scanner: The Brazilian Scenario

Gontijo¹,

Ferreira²,

Silva³

et al. 2020

Braz. J. Rad. Sci.

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This work aims to evaluate the quality control program for small animal PET (positron emission tomography) scanners in Brazil. A questionnaire focused on type of small animal PET scanner and quality assurance program was prepared and sent to all Brazilian preclinical molecular imaging services that participated in the first Brazilian microPET/SPECT/CT users meeting held at the Federal University of Rio de Janeiro. The answers were compiled and descriptive statistic was applied. There are six preclinical molecular imaging services using PET scanner in Brazil. Five are in the southeastern region and one in the southern region. All services have a single PET device, but most of them have hybrids modalities with CT (computed tomography) and/or SPECT (single photon emission computed tomography). All services have knowledge of the NEMA NU 4-2008 publication specific to small animal PET equipments. However, most of them do not have a quality assurance program in place for their imaging systems. There are few preclinical PET devices installed in Brazil and most of them are located at southeastern region. Regardless the absence of quality assurance program in many of the services, there is a consensus on the importance of quality control program implementation.

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“…At an activity concentration of 13.5 kBq/mL, the recovery coefficients for 18 (21). Considering that the organs of ostrich embryos are naturally larger than those of chicken embryos (e.g., liver size: 15 vs. 2 cm 3 ) (18,22), it is conceivable that imaging is comparable.…”

Section: Discussionmentioning

confidence: 99%

“…This aspect has to be evaluated in future studies. Obviously, because of the small gantry size of 98.6 mm, the small-animal device does not allow the investigation of ostrich eggs (21).…”

Section: Discussionmentioning

confidence: 99%

The Use of Ostrich Eggs for In Ovo Research: Making Preclinical Imaging Research Affordable and Available

et al. 2018

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In ovo studies are a valuable option in preclinical research, but imaging studies are severely limited by the costs of dedicated equipment needed for small-sized eggs. We sought to verify the feasibility of using larger, ostrich, eggs () for imaging on the PET/CT scanners used for routine clinical investigations. Ostrich eggs were incubated until shortly before hatching, prepared for intravitelline venous injection of contrast medium or radiotracer, and imaged using native CT, contrast-enhanced CT, and PET/CT. Any technical adaptations that were needed to improve the outcome were noted. Of the 34 eggs initially incubated, 12 became fully available for imaging of embryonal development. In ovo imaging with conventional PET/CT not only was feasible but also provided images of good quality, including on dynamic PET imaging. In ovo imaging with ostrich eggs and routine clinical scanners may allow broader application of this field of preclinical research, obviating costly dedicated equipment and reducing the number of animals needed for classic animal research. Further experiments are warranted to refine this novel approach, especially to reduce motion artifacts and improve monitoring of viability.

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Investigation of Image Reconstruction Parameters of the Mediso nanoScan PC Small-Animal PET/CT Scanner for Two Different Positron Emitters Under NEMA NU 4-2008 Standards

Abstract: A high regularization level at 52 iteration updates and 30 min acquisition time were found to optimize most of the figures of merit investigated.

Cited by 13 publications

References 20 publications

Performance of nanoScan PET/CT and PET/MR for quantitative imaging of 18F and 89Zr as compared with ex vivo biodistribution in tumor-bearing mice

Performance of nanoScan PET/CT and PET/MR for quantitative imaging of 18F and 89Zr as compared with ex vivo biodistribution in tumor-bearing mice

Quality control of small animal PET scanner: The Brazilian Scenario

The Use of Ostrich Eggs for In Ovo Research: Making Preclinical Imaging Research Affordable and Available

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