Investigation of MBL2 and NOS3 functional gene variants in suspected COVID-19 PCR (–) patients

Abstract

For COVID-19 (Coronavirus Disease-2019) cases, detecting host-based factors that predispose to infection is a very important research area. In this study, the aim is to investigate the MBL2 and NOS3 gene polymorphisms in COVID-19 patients with lung involvement, whose first nasopharyngeal PCR results were negative. Seventy-nine patients diagnosed with COVID-19 between April-June 2020 who were admitted to a university hospital, and 100 healthy controls were included. In the first statistical analysis performed between PCR-positive, CT-negative and PCR-negative, CT-positive patients; the AB of MBL2 genotype was significantly higher in the first group (p = 0.049). The B allele was also significantly higher in the same subgroup (p = 0.001). The absence of the AB genotype was found to increase the risk of CT positivity by 6.9 times. The AB genotype of MBL2 was higher in healthy controls (p = 0.006). The absence of the AB genotype was found to increase the risk of CT positivity; also, it can be used for early detection and isolation of patients with typical lung involvement who had enough viral loads, but whose initial PCR results were negative.

NOS3 (rs61722009) gene variants testing in prediction of COVID-19 pneumonia severity

“…In our study, the association of the 4a allele with the risk of severe COVID-19-associated pneumonia was determined. In contrast, in the study of Pehlivan et al no significant difference in the distribution of genotypes for the rs61722009 variant among patients diagnosed with COVID-19 (all patients had typical COVID-19 lung involvement in their initial CT) and healthy controls was found [ 17 ].…”

Molecular mechanism of the effect of Gegen Qinlian decoction on COVID-19 comorbid with diabetes mellitus based on network pharmacology and molecular docking: A review

“…[ 29 , 30 ] Noteworthily, 80% of diabetic patients die of thrombotic death, so F2 is also an important target for the prevention and treatment of diabetes. [ 31 ] Studies have found that polymorphisms in the NOS3 gene are closely related to susceptibility to COVID-19 [ 32 ] and susceptibility to vascular disease in patients with type 2 diabetes, [ 33 ] indicating this gene to be an important target for the treatment of both diseases.…”

Molecular Mechanisms Related to Responses to Oxidative Stress and Antioxidative Therapies in COVID-19: A Systematic Review

“…Nitric oxide is essential in COVID-19, as it develops inflammatory stress to prevent pulmonary hypertension [ 106 , 127 ]. Prooxidant eNOS enzyme is encoded by the NOS3 gene, which is highly polymorphic.…”

“…The A allele was higher in COVID-19 patients, whereas the B allele and the AB genotype were higher in individuals with negative tests. NOS enzyme catalyzes superoxide anion production and promotes nitric oxide synthesis [ 106 ].…”