Investigation of Cytotoxic T‐lymphocyte antigen 4 Polymorphisms in Gastric Cardia Adenocarcinoma

Abstract

To assess the potential effects of Cytotoxic T-lymphocyte antigen 4 (CTLA4) gene polymorphisms on susceptibility to gastric cardia adenocarcinoma (GCA), we genotyped four polymorphisms (rs733618 A>G, rs16840252 C>T, rs231775 G>A and rs3087243 G>A) in CTLA4 and calculated odds ratios (ORs) with the corresponding 95% confidence intervals (95% CIs) for the genotype and allele distributions between GCA cases and controls. The CTLA4 genotypes were determined by the polymerase chain reaction-ligase detection reaction (PCR-LDR) analysis in 330 GCA patients and 608 unrelated cancerfree controls. In this case-control study, there was no significant difference in the genotype and allele distributions of four CTLA4 polymorphisms between GCA patients and controls. However, haplotype association analysis indicated that compared with CTLA4 G rs733618 C rs16840252 G rs231775 C rs3087243 , CTLA4 G rs733618 C rs16840252 A rs231775 G rs3087243 and A rs733618 C rs16840252 G rs231775 A rs3087243 haplotypes conferred increased risks of GCA (OR = 6.46, 95% CI = 1.33-31.28; P = 0.012; both); however, CTLA4 A rs733618 C rs16840252Ars231775 G rs3087243 and A rs733618 T rs16840252 G rs231775 G rs3087243 haplotypes conferred decreased risks of GCA (P = 0.001 and P = 0.011, respectively). These results highlight that the rare CTLA4 haplotypes may affect the development of GCA in the Chinese population.

Investigation of BTLA tagging variants with risk of esophagogastric junction adenocarcinoma

“…The etiology of EGJA may be attribute to gene and environment factors. Recent evidence suggested that the variants of immune and inflammatory response related genes could alter the risk of cancer [21,[32][33][34][35]. Considering an important role of BTLA gene in immune, we chose BTLA tagging SNPs (rs16859629, rs1982809, rs2171513 and rs3112270) and explored their effects on the development of EGJA.…”

Programmed death-1 polymorphisms is associated with risk of esophagogastric junction adenocarcinoma in the Chinese Han population: A case-control study involving 2,740 subjects

“…The etiology of EGJA is very complicated. Accumulating evidences demonstrated that SNPs in some immune response genes might be associated with risk of cancer [6, 26–28]. In consideration of the vital role of PD-1 in tumor immunology, we chose PD-1 rs10204525 T>C, rs2227982 A>G, rs36084323 T>C and rs7421861 A>G polymorphisms to examine their potential roles in EGJA.…”

“…Although a number of studies have focused on the etiology of EGJA, it is not well understood. Of late, some studies reported the immune system might be implicated in the etiology of EGJA [5, 6]. …”

Relationship between <em>IGF2BP2</em> and <em>IGFBP3</em> polymorphisms and susceptibility to non-small-cell lung cancer: a case&ndash;control study in Eastern Chinese Han population

“…Whether the IGF2BP2 rs1470579 A>C, rs4402960 G>T and IGFBP3 rs2270628 C>T, rs3110697 G>A and rs6953668 G>A genotypes in controls conformed to Hardy–Weinberg equilibrium (HWE) was determined by an Internet-based calculator (http://ihg.gsf.de/cgi-bin/hw/hwa1.pl). 24–30 A P <0.05 (two-tailed) was accepted as the criterion of statistical signifi-cance. The relationship between IGF2BP2 rs1470579 A>C, rs4402960 G>T and IGFBP3 rs2270628 C>T, rs3110697 G>A and rs6953668 G>A polymorphisms with NSCLC susceptibility was assessed by crude/adjusted ORs and 95% CIs.…”