Investigation of human haemotropic Mycoplasma infections using a novel generic haemoplasma qPCR assay on blood samples and blood smears

Tasker, Séverine; Peters, Iain R.; Mumford, Andrew D; Day, Michael; Gruffydd-Jones, TJ; Day, S.; Pretorius, Anne-Marié; Birtles, Richard J.; Helps, Christopher R; Neimark, Harold

doi:10.1099/jmm.0.021691-0

Cited by 46 publications

(40 citation statements)

References 40 publications

(63 reference statements)

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“…(2) Bartonella henselae was evaluated by the method of Molia et al (2004). (3) General screening for hemoplasma DNA was done with a SYBR Green real-time PCR (Willi et al 2009), and positive samples were then tested with two hemoplasma group-specific TaqMan real-time PCRs (hemofelis vs. hemominutum groups; Tasker et al 2010).…”

Section: Methodsmentioning

confidence: 99%

Vector-Borne Agents Detected in Fleas of the Northern White-Breasted Hedgehog

Hornok

Földvári

Rigó

et al. 2014

Vector-Borne and Zoonotic Diseases

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This is the first large-scale molecular investigation of fleas from a geographically widespread and highly urbanized species, the northern white-breasted hedgehog. In this study, 759 fleas (the majority were Archaeopsylla erinacei) collected from 134 hedgehogs were molecularly analyzed individually or in pools for the presence of three groups of vector-borne pathogens. All flea samples were positive for rickettsiae: In two samples (1.5%) Rickettsia helvetica and in 10% of the others a novel rickettsia genotype were identified. Additionally, Bartonella henselae (the causative agent of cat scratch disease in humans) was demonstrated in one flea (0.7%), and hemoplasmas of the hemofelis group were identified in seven other samples (5.2%). The findings of vector-borne agents not detected before in A. erinacei fleas broaden the range of those diseases of veterinary-medical importance, of which hedgehogs may play a role in the epidemiology. This is the first large-scale molecular investigation of fleas from a geographically widespread and highly urbanized species, the northern white-breasted hedgehog. In this study, 759 fleas (the majority were Archaeopsylla erinacei) collected from 134 hedgehogs were molecularly analyzed individually or in pools for the presence of three groups of vector-borne pathogens. All flea samples were positive for rickettsiae: In two samples (1.5%) Rickettsia helvetica and in 10% of the others a novel rickettsia genotype were identified. Additionally, Bartonella henselae (the causative agent of cat scratch disease in humans) was demonstrated in one flea (0.7%), and hemoplasmas of the hemofelis group were identified in seven other samples (5.2%). The findings of vector-borne agents not detected before in A. erinacei fleas broaden the range of those diseases of veterinary-medical importance, of which hedgehogs may play a role in the epidemiology. DOI

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Section: Methodsmentioning

confidence: 99%

Vector-Borne Agents Detected in Fleas of the Northern White-Breasted Hedgehog

Hornok

Földvári

Rigó

et al. 2014

Vector-Borne and Zoonotic Diseases

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“…For preliminary screening of hemoplasma infections, universal PCR primers for the hemoplasmas' 16S rRNA gene were used, as described by Tasker et al [16]. Real-time PCR was performed using a SmartCycler instrument (Cepheid, Sunnyvale, CA, U.S.A.) with SYBR Premix Ex Taq (Code #RR041A, TaKaRa Bio., Otsu, Japan).…”

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confidence: 99%

Detection of Hemotropic Mycoplasmas in Free-Living Brown Sewer Rats (<i>Rattus norvegicus</i>)

et al. 2013

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ABSTRACT. The prevalence of hemotropic mycoplasmas in wild rodents is largely unknown. Here, we report the presence of hemoplasmas in blood samples collected from brown sewer rats (Rattus norvegicus) trapped during rodent control around an animal hospital in Morioka, Japan. We examined nine rats using real-time PCR and end-point PCR, and found one rat (11.1%) that was positive for a hemoplasma infection. The 16S rRNA gene and 16S to 23S rRNA intergenic spacer region of the hemoplasma detected in a wild-caught rat were amplified using PCR. The nucleotide sequences of the PCR products were further determined and compared to those of other hemoplasmas. Our examinations revealed the presence of a hemoplasma that has not previously been described in rodents. The pathogenic traits of this hemoplasma remain unexplored.

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“…to invade erythrocytes exists in the literature; the possible concomitant presence of other pathogens able to invade erythrocytes cannot be ruled out. A possibility is the presence of hemoplasma, which are wall-less erythrocytic bacteria unable to be cultured in vitro, classified within the genus Mycoplasma, which have been described in human and animal infections [11]. However, an in silico analysis showed that the 16S rRNA universal primers used were able to amplify a fragment of 1,433 bp of the 16S rRNA gene of hemoplasma species (i.e., Candidatus Mycoplasma haemominutum, GeneBank access NC_021007.1).…”

Section: Discussionmentioning

confidence: 99%

An unidentified cluster of infection in the Peruvian Amazon region

Cornejo

Gomes

Suárez

et al. 2015

J Infect Dev Ctries

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Introduction: Bartonella bacilliformis is the etiological agent of Carrion’s disease, which is a neglected disease linked to people in low-socioeconomic populations in Andean valleys. An outbreak of B. bacilliformis was reported in a rural area of the Peruvian Amazon region. The aim of this study was to characterize this outbreak using molecular techniques. Methodology: Fifty-three blood samples from patients diagnosed with Carrion’s disease were analyzed by molecular tools, using both a Bartonella-specific polymerase chain reaction (PCR) and an universal PCR, both based on 16S rRNA gene amplification. Additional water samples from the area were also analyzed. Results: Unexpectedly, the samples were positive only when the universal PCR was used. Although environmental contamination cannot be ruled out, the results showed that Sphingomonas faeni was the possible causative agent of this outbreak, and that water was the most feasible infection source. Conclusions: Diagnosis by clinical criteria or microscopy may lead to misdiagnosis. There is a need to include molecular tools in the routine diagnosis of febrile syndromes, including Carrion’s disease.

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Investigation of human haemotropic Mycoplasma infections using a novel generic haemoplasma qPCR assay on blood samples and blood smears

Cited by 46 publications

References 40 publications

Vector-Borne Agents Detected in Fleas of the Northern White-Breasted Hedgehog

Vector-Borne Agents Detected in Fleas of the Northern White-Breasted Hedgehog

Detection of Hemotropic Mycoplasmas in Free-Living Brown Sewer Rats (<i>Rattus norvegicus</i>)

An unidentified cluster of infection in the Peruvian Amazon region

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