Investigation of hemicellulosic hydrolysate inhibitor resistance and fermentation strategies to overcome inhibition in non-saccharomyces species

Soares, Lauren B.; Bonan, C.I.D.G.; Biazi, Luiz Eduardo; Dionísio, S.R.; Bonatelli, Maria Letícia; Andrade, Albuquerque; Renzano, E.C.; Costa, Aline Carvalho da; Ienczak, Jaciane Lutz

doi:10.1016/j.biombioe.2020.105549

Cited by 41 publications

(21 citation statements)

References 24 publications

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“…However, the above concentrations are lower than the respective reported as inhibitory for P. stipitis growth, when furans or acetic acid were added separately, in a xylose-rich substrate [ 36 ]. It should be mentioned, however, that the simultaneous presence of all the above might have a synergistic toxic effect, causing partial or total inhibition to the same yeast, even though this did not happen when they are added separately [ 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

Αντωνοπούλου¹

2020

Molecules

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The effect of thermal, acid and alkali pretreatment methods on biological hydrogen (BHP) and bioethanol production (BP) from grass lawn (GL) waste was investigated, under different process schemes. BHP from the whole pretreatment slurry of GL was performed through mixed microbial cultures in simultaneous saccharification and fermentation (SSF) mode, while BP was carried out through the C5yeast Pichia stipitis, in SSF mode. From these experiments, the best pretreatment conditions were determined and the efficiencies for each process were assessed and compared, when using either the whole pretreatment slurry or the separated fractions (solid and liquid), the separate hydrolysis and fermentation (SHF) or SSF mode, and especially for BP, the use of other yeasts such as Pachysolen tannophilus or Saccharomyces cerevisiae. The experimental results showed that pretreatment with 10 gH2SO4/100 g total solids (TS) was the optimum for both BHP and BP. Separation of solid and liquid pretreated fractions led to the highest BHP (270.1 mL H2/g TS, corresponding to 3.4 MJ/kg TS) and also BP (108.8 mg ethanol/g TS, corresponding to 2.9 MJ/kg TS) yields. The latter was achieved by using P. stipitis for the fermentation of the hydrolysate and S. serevisiae for the solid fraction fermentation, at SSF.

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Section: Resultsmentioning

confidence: 99%

Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

Αντωνοπούλου¹

2020

Molecules

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“…According to the authors, Barnettozyma californica adapted to the concentrated hydrolysate exhibited a highest increase in ethanol yield and productivity than in the non-concentrated hydrolysate, as well as in growth rate. The need for adaptation in face of increased toxicity was also observed for S. stipitis grown in semi-de ned media containing glucose and xylose and increasing concentrations of acetic acid [41].…”

Section: Effect Of Short-term Adaptation On Bioconversion Of Xylose To Xylitolmentioning

confidence: 83%

Short-term Adaptation Strategy Improved Xylitol Production by Candida guilliermondii on Sugarcane Bagasse Hemicellulosic Hydrolysate

et al. 2021

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One of the major bottlenecks of the biotechnological production of xylitol by pentose-fermenting yeasts is the presence of toxic compounds in the hemicellulosic hydrolysates, which inhibit the bioconversion of xylose into xylitol. In this work, short-term adaptation was evaluated as a strategy to minimize the toxicity of the sugarcane bagasse hemicellulosic hydrolysate to Candida guilliermondii FTI 20037. Yeast adaptation improved xylose assimilation as well as xylitol production. The bene cial effects of adaptation were more pronounced in the hydrolysate with higher concentration of toxic compounds, leading to an increase of 62.5% in the xylitol volumetric productivity in comparison to the use of nonadapted cells. In this condition, it was also veri ed the reduction of glycerol production (about 102%), a by-product formed as consequence of cellular stress, indicating a greater tolerance of adapted cells to the toxicity of hydrolysates. Short-term adaptation proved to be a promising strategy to improve considerably the microbial tolerance and overcome the toxicity of hydrolysates.

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“…1a) were approximately 20 g/L at the beginning of B1. Low cell growth was observed in the five SBF with free cells, and all SBF showed constancy for the initial cell concentration (approximately 20 g/L), probably due to O2 limitation caused by bioreactor choice (shaker) [20,38]. It is important to highlight that there was no purge in SBF with free cells and all the cells present in the previous batch were transferred to a subsequent batch.…”

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 96%

“…Spathaspora passalidarum has demonstrated great potential in the production of E2G, mainly due to its capacity to consume xylose at high rates [19][20][21][22] and present better performance in hemicellulosic hydrolysates when compared to other xylose-fermenters yeasts [19][20][21][22]. Despite this, there are still challenges in the use of this microorganism in processes for obtaining E2G, like the need for additional nutrients when using hemicellulosic hydrolysates, the necessity of aeration, and the need for cell robustness against hemicellulosic hydrolysate inhibitors [23].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

et al. 2021

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The immobilization of S. passalidarum in calcium alginate beads for second-generation ethanol production (E2G) was evaluated in a medium that simulated a hemicellulosic hydrolysate of sugarcane bagasse pretreated with diluted sulfuric acid in terms of sugars composition. Three sets of sequential batch fermentations (SBF) were carried out with free cells, or immobilized cells in high (HSC) and moderate (MSC) initial sugar concentration (120 and 70 g/L, respectively). SBF were characterized by five consecutive batches performed in shaker, at 30 °C and 110 rpm. Better results were observed for the SBF with immobilized cells in MSC medium when compared to HSC (Y'P/S of 0.27 ± 0.02 and 0.19 ± 0.03 g/g, respectively), in the second batch cycle. The value for YP/S in MSC was similar to the obtained with free cells (0.30 ± 0.02 to 0.33 ± 0.02 g/g). However, QP was lower for MSC, reaching 0.81 ± 0.04 g/L.h in in the second batch, while for free cells the QP varied from 1.06 ± 0.02 to 1.16 ± 0.22 g/L.h. A technique for determining the concentration of immobilized cells in the alginate beads was applied, which made it possible to determine the specific rates for the SBF performed. According to the results obtained, it was possible to demonstrate that S. passalidarum can be immobilized in calcium alginate and reuse through SBF, with performance similar to free cells, which can be a good strategy for fermentation of hemicellulosic hydrolysates.

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Investigation of hemicellulosic hydrolysate inhibitor resistance and fermentation strategies to overcome inhibition in non-saccharomyces species

Cited by 41 publications

References 24 publications

Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

Short-term Adaptation Strategy Improved Xylitol Production by Candida guilliermondii on Sugarcane Bagasse Hemicellulosic Hydrolysate

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

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