Investigation of HBV DNA in HBsAg positive patients

Abstract

Hepatitis B virus is an important health problem which about 400 million people are infected chronically and 500,000 to 1,200,000 people die every year due to HBVrelated diseases. In the study we aimed to investigate the HBV DNA results in HBsAg positive patients. HBsAg positivity was detected by ELISA method in patients who were admitted to our hospital with suspicion of hepatitis. HBV DNA was performed by real time PCR. In this context, the presence of HBV DNA in serum sample results of 2437 HBsAg-positive patients which were sent to our Microbiology laboratory between August 2014 and August 2015 was retrospectively examined. HBV DNA was positive in 1037 (42.5%) and 1400 (57.5%) of the HBsAg positive cases. 42.2% of male patients' and 43% of female patients' HBV DNA was positive. HBV DNA positivity was detected in 42% of adult patients and 55% of children in child age group. As a result; we suggest that viral replication should be demonstrated in HBsAgpositive cases and HBV DNA level should be determined for follow-up the antiviral therapy.

Occult Hepatitis B Virus Infection among β-Thalassemia Major Patients in Ahvaz City, Iran

“…It is interesting to note that all four HBsAg-positive patients were HBV DNA negative, which supports the idea that there is no correlation between HBsAg positivity and HBV DNA. [21][22][23] The prevalence of occult HBV infection has been studied in different parts of the world and among various groups; however, there are conflicting with results even in a similar group. As an illustration, OBI prevalence among hemodialysis patients was 1.5% (Turkey), 24 0.5% (Iran), 25 0% (Germany), 26 1.3% (Japan), 27 and 0% (Italy) 28 ; in hemophilia patients was 1.73% in Pakistan, 29 9.3% in Iran, 30 and 0% in Polish 31 ; among thalassemia patients was 31.4% (India), 6 32.5% (Egypt), 5 and 0% (Iran) 13,14 ; in individuals with HCC was 30% (Japan), 32 38.5% (Korea), 33 50% (Egypt), 34 and 70.4% (China) 35 ; among blood donors in different parts of the world was 3.7% (Egypt), 36 4.71% (India), 37 1.98% (Colombia), 38 0.3% (Lebanon), 39 0% (Iran), 8 and 7.4% (Libya).…”

Investigation of Hepatitis B virus DNA among HBsAg positive patients in Kabul, Afghanistan

“…More than 350 million people worldwide have chronic HBV 3 . And annually about 500,000 -1200,000 people die from various HBV-related illnesses, and on average, 5% of people infected with acute hepatitis B become chronic 4 . The risk of developing chronic infections in children under the age of one year reaches 90% and about 25% of them die before adolescence due to cancer and liver cirrhosis 5 .…”

“…Real time PCR is a simple and fast that allows the quantification of viral DNA 4 . The aim of this study is to investigate the presence of hepatitis B virus DNA by Real-time PCR in HBsAg positive patients and the risk factor for HBV activation among HbsAg positive patients.…”