Investigation of Gluthatione S-Transferase Variants in a Healthy Population in Goiânia-Go

Abstract

The study was performed according approved by the ethics committee of Federal University of Goias (approval CEP: 895.552). In the current survey, 100 patients (35M/65F) of both genders and aged 18-90 (M: 30.2) years old considered healthy and practicing physical activity 2 to 3 times a week, were enrolled after receiving their informed consent. Sample collection and DNA analyzes Peripheral blood (5mL) was collected in EDTA vacutainer tubes from all participating individuals after obtaining their written consent. Until the DNA extraction blood DNA collections was store a-20°C. Genomic DNA extraction was performed from whole blood using a Purelink Genomic DNA Kit (Invitrogen, Life Technologies Inc.; USA) and the concentration was measured using a NanoDrop™ 2000/2000c Spectrophotometers (Thermo Scientific, USA).