Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules

Kamiya, Kanichi; Arisaka, Chika; Suzuki, Masato

doi:10.3390/mi12020133

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…The preparation of conventional floating lipid bilayers 15 is typically based on the Langmuir-Blodgett (LB) and/or Langmuir Schaefer (LS) techniques 9,41 . This approach obviously cannot be taken for the deposition of lipid bilayers onto fluid interfaces and the vesicle fusion technique 42,43 has to be employed instead.…”

Section: Dmpc-dmpg Bilayer Supported By a Dppe-dstap Monolayermentioning

confidence: 99%

Characterization of Lipid Bilayers Adsorbed to Functionalized Air/Water Interfaces

Pusterla

Scoppola

Appel

et al. 2022

Preprint

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Lipid bilayers immobilized in planar geometries, such as solid-supported or "floating" bilayers, have enabled detailed studies of biological membranes with numerous experimental techniques, notably x-ray and neutron reflectometry. However, the presence of a solid support also has disadvantages as it complicates the use of spectroscopic techniques as well as surface rheological measurements that would require surface deformations. Here, in order to overcome these limitations, we investigate lipid bilayers adsorbed to inherently soft and experimentally well accessible air/water interfaces that are functionalized with Langmuir monolayers of amphiphiles. The bilayers are characterized with ellipsometry, X-ray scattering, and X-ray fluorescence. Grazing-incidence X-ray diffraction reveals that lipid bilayers in a chain-ordered state can have significantly different structural features than regular Langmuir monolayers of the same composition. Our results suggest that bilayers at air/water interfaces may be well suited for fundamental studies in the field of membrane biophysics.

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Section: Dmpc-dmpg Bilayer Supported By a Dppe-dstap Monolayermentioning

confidence: 99%

Characterization of Lipid Bilayers Adsorbed to Functionalized Air/Water Interfaces

Pusterla

Scoppola

Appel

et al. 2022

Preprint

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“…In contrast, liposomes formed by the pulsed-jet flow method have long-term stability (up to one week). The flip-flop phenomenon and membrane protein reconstitution have been investigated on the asymmetric lipid vesicles formed by this method [ 64 , 65 ]. Modification of the double-well device allows for the formation of nano-sized asymmetric lipid vesicles [ 66 ], automatic formation of liposomes [ 67 , 68 ], and the formation of vesicles-in-a-vesicle structures that mimic the asymmetric lipid composition of plasma membranes and intracellular vesicle membranes [ 69 ].…”

Section: Formation Of Cell-sized Lipid Vesiclesmentioning

confidence: 99%

Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes

Tosaka

Kamiya

2023

IJMS

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Membrane proteins play an important role in key cellular functions, such as signal transduction, apoptosis, and metabolism. Therefore, structural and functional studies of these proteins are essential in fields such as fundamental biology, medical science, pharmacology, biotechnology, and bioengineering. However, observing the precise elemental reactions and structures of membrane proteins is difficult, despite their functioning through interactions with various biomolecules in living cells. To investigate these properties, methodologies have been developed to study the functions of membrane proteins that have been purified from biological cells. In this paper, we introduce various methods for creating liposomes or lipid vesicles, from conventional to recent approaches, as well as techniques for reconstituting membrane proteins into artificial membranes. We also cover the different types of artificial membranes that can be used to observe the functions of reconstituted membrane proteins, including their structure, number of transmembrane domains, and functional type. Finally, we discuss the reconstitution of membrane proteins using a cell-free synthesis system and the reconstitution and function of multiple membrane proteins.

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“…The inkjet printing method starts from a planar, asymmetric bilayer formed at the contact of two aqueous droplets in an oily phase. From this bilayer, vesicles are ejected by the printing pulse [ 36 , 37 , 38 , 39 , 40 ].…”

Section: Navigating the Preparation Of Asymmetric Model Membranesmentioning

confidence: 99%

A Guide to Your Desired Lipid-Asymmetric Vesicles

Krompers

Heerklotz

2023

Membranes

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Liposomes are prevalent model systems for studies on biological membranes. Recently, increasing attention has been paid to models also representing the lipid asymmetry of biological membranes. Here, we review in-vitro methods that have been established to prepare free-floating vesicles containing different compositions of the classic two-chain glycero- or sphingolipids in their outer and inner leaflet. In total, 72 reports are listed and assigned to four general strategies that are (A) enzymatic conversion of outer leaflet lipids, (B) re-sorting of lipids between leaflets, (C) assembly from different monolayers and (D) exchange of outer leaflet lipids. To guide the reader through this broad field of available techniques, we attempt to draw a road map that leads to the lipid-asymmetric vesicles that suit a given purpose. Of each method, we discuss advantages and limitations. In addition, various verification strategies of asymmetry as well as the role of cholesterol are briefly discussed. The ability to specifically induce lipid asymmetry in model membranes offers insights into the biological functions of asymmetry and may also benefit the technical applications of liposomes.

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Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules

Cited by 7 publications

References 28 publications

Characterization of Lipid Bilayers Adsorbed to Functionalized Air/Water Interfaces

Characterization of Lipid Bilayers Adsorbed to Functionalized Air/Water Interfaces

Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes

A Guide to Your Desired Lipid-Asymmetric Vesicles

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