Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G

Saha, Jaba; Roy, Aidan; Dey, Dibyendu; Chakraborty, Santanu; Bhattacharjee, D.; Paul, Piyali; Hussain, Syed Arshad

doi:10.1016/j.saa.2015.04.027

Cited by 46 publications

(21 citation statements)

References 35 publications

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“…Examples of FRET between FL and several types of rhodamines have been reported for dye concentrations of the same order as Fig. 8b [in particular, FRET in FL/ rhodamine 6G mixtures was investigated by Saha et al (2015)].…”

Section: Temperature Calibration and Dye Concentrationsmentioning

confidence: 99%

Spatially and temporally resolved measurements of the temperature inside droplets impinging on a hot solid surface

et al. 2017

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Section: Temperature Calibration and Dye Concentrationsmentioning

confidence: 99%

Spatially and temporally resolved measurements of the temperature inside droplets impinging on a hot solid surface

et al. 2017

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“…On one hand, many organic dyes and quantum dots (QDs) have proven to be efficient FRET donors in the last several years. QDs have received considerable attention as fluorescence labels in the FRET biosensor due to its high brightness, multifunctional-group accessibility, long fluorescence lifetime and strong photoluminescence (PL) [22,23,24]. On the other hand, gold nanoparticles (AuNPs) can be considered as promising candidates for acceptors of the FRET system due to their extremely high extinction coefficient and super biocompatibility [25,26,27,28].…”

Section: Introductionmentioning

confidence: 99%

“…Growing interests in bioassays, providing transduction of bioinformation to optical and electronic signals have recently been observed in conjunction with stimulating developments in the synthesis of highly efficient quantum-dots and functionalized AuNPs. To the best of our knowledge, all the previously reported FRET biosensors have been focused on the traditional FRET mode in liquid [22,23,24,25,26,27]. However, those optical biosensors are limited in sensitivity and application since the surface area is small and the interaction between biomolecules and the electromagnetic field is not optimal.…”

Section: Introductionmentioning

confidence: 99%

Efficient Fluorescence Resonance Energy Transfer between Quantum Dots and Gold Nanoparticles Based on Porous Silicon Photonic Crystal for DNA Detection

Zhang

Jia

2017

Sensors

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A novel assembled biosensor was prepared for detecting 16S rRNA, a small-size persistent specific for Actinobacteria. The mechanism of the porous silicon (PS) photonic crystal biosensor is based on the fluorescence resonance energy transfer (FRET) between quantum dots (QDs) and gold nanoparticles (AuNPs) through DNA hybridization, where QDs act as an emission donor and AuNPs serve as a fluorescence quencher. Results showed that the photoluminescence (PL) intensity of PS photonic crystal was drastically increased when the QDs-conjugated probe DNA was adhered to the PS layer by surface modification using a standard cross-link chemistry method. The PL intensity of QDs was decreased when the addition of AuNPs-conjugated complementary 16S rRNA was dropped onto QDs-conjugated PS. Based on the analysis of different target DNA concentration, it was found that the decrease of the PL intensity showed a good linear relationship with complementary DNA concentration in a range from 0.25 to 10 μM, and the detection limit was 328.7 nM. Such an optical FRET biosensor functions on PS-based photonic crystal for DNA detection that differs from the traditional FRET, which is used only in liquid. This method will benefit the development of a new optical FRET label-free biosensor on Si substrate and has great potential in biochips based on integrated optical devices.

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“…Fluorescently labeled nhTMEM16 variants were reconstituted into proteoliposomes as described previously (6,8,31). Briefly, lipid stock solutions were mixed to obtain 20 mg of lipids (ϳ27 mol) in a molar ratio of three parts (3 POPE, 1 POPG) to one part 60% egg PC and evaporated to dryness under a gentle stream of nitrogen.…”

Section: Proteoliposomesmentioning

confidence: 99%

“…Using E and R 0 values, the distance between the donor and acceptor can be calculated by Equation 8 (31). r ϭ R 0 /͑͑1/E͒ Ϫ 1͒ 1/6 (Eq.…”

Section: Distance Between the N Terminus Of One Monomer And The C Termentioning

confidence: 99%

Structural mapping of fluorescently-tagged, functional nhTMEM16 scramblase in a lipid bilayer

Andra

Dorsey

Royer

et al. 2018

Journal of Biological Chemistry

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Most members of the TransMEMbrane protein 16 (TMEM16) family are Ca-regulated scramblases that facilitate the bidirectional movement of phospholipids across membranes necessary for diverse physiological processes. The nhTMEM16 scramblase (from the fungus ) is a homodimer with a large cytoplasmic region and a hydrophilic, membrane-exposed groove in each monomer. The groove provides the transbilayer conduit for lipids, but the mechanism by which Ca regulates it is not clear. Because fusion of large protein tags at either the N or C terminus abolishes nhTMEM16 activity, we hypothesized that its cytoplasmic portion containing both termini may regulate lipid translocation via a Ca-dependent conformational change. To test this hypothesis, here we used fluorescence methods to map key distances within the nhTMEM16 homodimer and between its termini and the membrane. To this end, we developed functional nhTMEM16 variants bearing an acyl carrier protein (ACP) tag at one or both of the termini. These constructs were fluorescently labeled by ACP synthase-mediated insertion of CoA-conjugated fluorophores and reconstituted into vesicles containing fluorescent lipids to obtain the distance of closest approach between the labeled tag and the membrane via FRET. Fluorescence lifetime measurements with phasor analysis were used to determine the distance between the N and C termini of partnering monomers in the nhTMEM16 homodimer. We now report that the measured distances do not vary significantly between Ca-replete and EGTA-treated samples, indicating that whereas the cytoplasmic portion of the protein is important for function, it does not appear to regulate scramblase activity via a detectable conformational change.

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Investigation of Fluorescence Resonance Energy Transfer between Fluorescein and Rhodamine 6G

Cited by 46 publications

References 35 publications

Spatially and temporally resolved measurements of the temperature inside droplets impinging on a hot solid surface

Spatially and temporally resolved measurements of the temperature inside droplets impinging on a hot solid surface

Efficient Fluorescence Resonance Energy Transfer between Quantum Dots and Gold Nanoparticles Based on Porous Silicon Photonic Crystal for DNA Detection

Structural mapping of fluorescently-tagged, functional nhTMEM16 scramblase in a lipid bilayer

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