Abstract:Femtosecond (<300fs fwhm) measurements of fluorescence decay and quenching of Tryptophan (Trp) were performed in a variety of proteins, including GB1, Thioredoxin (both wild type from two species and a human Trx mutant with a single Trp), Cyanovirin and Interleukin-1beta using an upconversion spectrophotofluorometer combined with a time correlated single photon counting apparatus to span the ~200fs to 20ns time scale. Trp is subject both to ultrafast quenching in proteins and spectral energy loss coupled to n… Show more
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