Investigation of EROD, CYP1A immunopositive proteins and SOD in haemocytes of Chamelea gallina and their role in response to B[a]P

Monari, Marta; Foschi, Jurgen; Matozzo, Valerio; Marin, María Gabriella; Fabbri, Margherita; Rosmini, Roberto; Serrazanetti, Gian Paolo

doi:10.1016/j.cbpc.2008.09.008

Cited by 15 publications

(11 citation statements)

References 93 publications

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“…Many studies have examined total microsomal cytochrome P450 content, levels of CYP enzyme activity, or CYP protein by immunoassay in bivalve mollusks exposed to chemicals (Canova et al, 1998; Livingstone, 1998; Livingstone et al, 1989; Monari et al, 2007; Monari et al, 2009; Shaw et al, 2002; Snyder et al, 2001; Sole and Livingstone, 2005; Wootton et al, 1995). The interpretation of those measurements has been complicated by low catalytic activity of microsomal enzymes with diagnostic substrates for CYP1A-like activity, such as ethoxyresorufin (ethoxyresorufin-O-deethylase activity, EROD) and benzo[a]pyrene (benzo[a]pyrene hydroxylase; BPH), and lack of specific cross-reactivity of antibodies for CYP1As, (Chaty et al, 2004; Jonsson et al, 2006a; Jonsson et al, 2006b).…”

Section: Introductionmentioning

confidence: 99%

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

et al. 2013

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Various sequencing projects over the last several years have aided the discovery of previously uncharacterized invertebrate sequences, including new cytochrome P450 genes (CYPs). Here we present data on the identification and characterization of two CYP1-like and three CYP3-like genes from the bivalve mollusk Mytilus edulis, and assess their potential as biomarkers based on their responses to several known vertebrate aryl hydrocarbon receptor (AHR) agonists. Quantitative real-time PCR was used to measure CYP transcript levels in digestive gland, labial palps, adductor muscle, gill, foot, and different regions of the mantle. Levels of both CYP1-like genes were highest in digestive gland, whereas labial palps had the highest expression levels of the three CYP3-like genes followed by digestive gland and outer margin of the mantle. Mussels were exposed by injection to the AHR agonists, β-naphthoflavone (BNF; 25 μg.g−1), 3,3′,4,4′,5-polychlorinated biphenyl (PCB126; 2 μg.g−1), or 6-formylindolo[3,2-b]carbazole (FICZ; 0.1 μg.g−1), or to Aroclor 1254 (a mixture of PCBs; 50 μg.g−1) for 24 hours, followed by CYP expression analysis. There was no statistically significant change in expression of either of the CYP1-like genes after exposure to the various AHR agonists. The CYP3-like-1 gene was significantly up-regulated by BNF in gill tissues and the CYP3-like-2 gene was up-regulated in digestive gland by PCB126 and in gill tissue by BNF. These results suggest that distinct mechanisms of CYP gene activation could be present in M. edulis, although the importance of the CYP1-like and CYP3-like genes for xenobiotic and endogenous lipids biotransformation requires additional investigation.

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Section: Introductionmentioning

confidence: 99%

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

et al. 2013

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“…In the control animals (with and without acetone), B[a]P levels were not detectable (0.2 ng/g w/ w was the instrument detection limit) as widely described in Monari et al [18]. We are not making further considerations on these data since they are out of the aim of this work.…”

Section: B[a]p Levelsmentioning

confidence: 57%

“…HSP analyses were performed on digestive gland extracts obtained from three pools of 10 clams (n ¼ 30) for each collecting point (24 h, 7 and 12 days) of the three experimental condition. B[a]P level analyses were carried out on a second set of whole soft tissue from 30 animals for each collecting point of the three experimental condition (three pools/condition) [18].…”

Section: Sampling and Experimental Proceduresmentioning

confidence: 99%

2-DE proteomic analysis of HSP70 in mollusc Chamelea gallina

Foschi

Matozzo

Rosmini

et al. 2011

Fish & Shellfish Immunology

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“…the presence of CYP1A-like genes and their inducibility in response to environmental contaminants have been continuously suggested in diverse invertebrates (Chaty et al, 2004;Grøsvik et al, 2006;Jonsson et al, 2006;Monari et al, 2007Monari et al, , 2009Zanette et al, 2010Zanette et al, , 2013. Thus, the inducibility of AhR-CYP signaling pathway genes in response to EDC exposure is controversial and requires in-depth study for a better understanding of how an AhR-CYP signaling pathway might respond to EDCs.…”

Section: Discussionmentioning

confidence: 97%

Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus

et al. 2015

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Investigation of EROD, CYP1A immunopositive proteins and SOD in haemocytes of Chamelea gallina and their role in response to B[a]P

Cited by 15 publications

References 93 publications

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

Identification and expression of multiple CYP1-like and CYP3-like genes in the bivalve mollusk Mytilus edulis

2-DE proteomic analysis of HSP70 in mollusc Chamelea gallina

Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus

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